Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera

Detalhes bibliográficos
Autor(a) principal: Celeste, Beatriz J.
Data de Publicação: 1988
Outros Autores: Guimarães, M. Carolina S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28562
Resumo: Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. Growth was more abundant for culture medium adapted cells; degenerate cells in small numbers as well as dead ones were present from day 5 for promastigotes adapted to liquid medium and from day 3 for newly adapted cells. Synthesis of surface antigens differed according to length of cell culture as assessed by the titer of five mucocutaneous leishmaniasis sera on subsequent days. Five days of culture for cells already adapted to the culture medium and 3 days for newly adapted ones were judged to be the best for the preparation of immunofluorescence antigens.
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spelling Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera Curvas de crescimento de proniastigotas de Leishmania braziliensis braziliensis e expressão de antígenos de superfície antes e depois da adaptação ao meio de drosófila de Schneider Leishmania braziliensis braziliensisCultureAntigen Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. Growth was more abundant for culture medium adapted cells; degenerate cells in small numbers as well as dead ones were present from day 5 for promastigotes adapted to liquid medium and from day 3 for newly adapted cells. Synthesis of surface antigens differed according to length of cell culture as assessed by the titer of five mucocutaneous leishmaniasis sera on subsequent days. Five days of culture for cells already adapted to the culture medium and 3 days for newly adapted ones were judged to be the best for the preparation of immunofluorescence antigens. Promastigotas de Leishmania braziliensis braziliensis (MHom/BR/75/M2903) foram cultivadas em meio de Schneider de cultura de drosófilas. Em um tipo de experiência as formas promastigotas já haviam sido previamente adaptadas ao meio por passagens consecutivas enquanto que no segundo as promastigotas foram cultivadas em meio bifásico e depois transferidas para o meio liqüido. O crescimento foi mais abundante nas células já adaptadas ao meio de cultura; células degeneradas e mortas, em pequenos números, estavam presentes desde o dia 5 para promastigotas adaptadas ao meio liqüido e desde o dia 3 para células recentemente adaptadas. A síntese de antígenos de superfície diferiu de acordo com o modo como as células eram cultivadas, como verificado pelo título de 5 soros de leishmaniose mucocutânea. O melhor período para a preparação de antígenos de imunofluorescência foi considerado o dia 5 para promastigotas adaptadas ao meio de cultivo e o dia 3 para células recentemente adaptadas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1988-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28562Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 2 (1988); 63-67 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 2 (1988); 63-67 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 2 (1988); 63-67 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28562/30415Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCeleste, Beatriz J.Guimarães, M. Carolina S.2012-07-02T01:04:31Zoai:revistas.usp.br:article/28562Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:17.857035Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
Curvas de crescimento de proniastigotas de Leishmania braziliensis braziliensis e expressão de antígenos de superfície antes e depois da adaptação ao meio de drosófila de Schneider
title Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
spellingShingle Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
Celeste, Beatriz J.
Leishmania braziliensis braziliensis
Culture
Antigen
title_short Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
title_full Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
title_fullStr Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
title_full_unstemmed Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
title_sort Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
author Celeste, Beatriz J.
author_facet Celeste, Beatriz J.
Guimarães, M. Carolina S.
author_role author
author2 Guimarães, M. Carolina S.
author2_role author
dc.contributor.author.fl_str_mv Celeste, Beatriz J.
Guimarães, M. Carolina S.
dc.subject.por.fl_str_mv Leishmania braziliensis braziliensis
Culture
Antigen
topic Leishmania braziliensis braziliensis
Culture
Antigen
description Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. Growth was more abundant for culture medium adapted cells; degenerate cells in small numbers as well as dead ones were present from day 5 for promastigotes adapted to liquid medium and from day 3 for newly adapted cells. Synthesis of surface antigens differed according to length of cell culture as assessed by the titer of five mucocutaneous leishmaniasis sera on subsequent days. Five days of culture for cells already adapted to the culture medium and 3 days for newly adapted ones were judged to be the best for the preparation of immunofluorescence antigens.
publishDate 1988
dc.date.none.fl_str_mv 1988-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28562
url https://www.revistas.usp.br/rimtsp/article/view/28562
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28562/30415
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 2 (1988); 63-67
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 2 (1988); 63-67
Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 2 (1988); 63-67
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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