Experimental infection and horizontal transmission of Bartonella henselae in domestic cats

Detalhes bibliográficos
Autor(a) principal: ZANUTTO, Marcelo de Souza
Data de Publicação: 2001
Outros Autores: MAMIZUKA, Elza Masae, RAIZ-JÚNIOR, Roberto, LIMA, Thais Martins de, DIOGO, Constância Lima, OKAY, Thelma Suely, HAGIWARA, Mitika Kuribayashi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30542
Resumo: In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed.
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spelling Experimental infection and horizontal transmission of Bartonella henselae in domestic cats Infecção experimental e transmissão horizontal de Bartonella henselae em gatos domésticos CatFelinesBartonella henselaeBartonella quintanaHemoculturePolymerase Chain ReactionIndirect immunofluorescence In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed. Procurou-se verificar a possibilidade de transmissão horizontal de B. henselae em 5 felinos confinados, dentre os quais apenas um foi inoculado experimentalmente por via subcutânea (SC) com 10(5) UFC. Todos os felinos apresentavam infestação por pulgas. Para a avaliação da infecção foram utilizados: isolamento bacteriano (hemocultura), detecção de DNA específico pela Reação em Cadeia da Polimerase (PCR), com os protocolos HSP, FTSZ e BH-PCR, e a pesquisa de anticorpos específicos por Imunofluorescência Indireta (IFI). Os protocolos da PCR foram também utilizados para a caracterização do isolado da hemocultura. A inoculação de B. henselae resultou na infecção assintomática do animal inoculado, comprovada através da soroconversão e de bacteremia de 4 meses de duração, com o isolamento da bactéria na hemocultura. Todos os animais contactantes apresentaram bacteremia 6 meses após a data de inoculação experimental. No entanto, não apresentaram reação de IFI positiva. Em nenhum momento foi possível detectar DNA de B. henselae no sangue circulante, com as PCR utilizadas. Não obstante, a PCR possibilitou a identificação da bactéria isolada como sendo do gênero Bartonella (HSP e FTSZ-PCR) e espécie henselae (BH-PCR). Conclui-se que o isolamento bacteriano por meio da hemocultura constitui-se no método mais eficiente para a identificação dos felinos infectados e bacterêmicos. Estes resultados também evidenciam a possibilidade do papel das pulgas na transmissão de B. henselae em gatos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30542Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 257-261 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 257-261 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 257-261 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30542/32426Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessZANUTTO, Marcelo de SouzaMAMIZUKA, Elza MasaeRAIZ-JÚNIOR, RobertoLIMA, Thais Martins deDIOGO, Constância LimaOKAY, Thelma SuelyHAGIWARA, Mitika Kuribayashi2012-07-07T13:35:16Zoai:revistas.usp.br:article/30542Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:19.676993Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
Infecção experimental e transmissão horizontal de Bartonella henselae em gatos domésticos
title Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
spellingShingle Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
ZANUTTO, Marcelo de Souza
Cat
Felines
Bartonella henselae
Bartonella quintana
Hemoculture
Polymerase Chain Reaction
Indirect immunofluorescence
title_short Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
title_full Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
title_fullStr Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
title_full_unstemmed Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
title_sort Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
author ZANUTTO, Marcelo de Souza
author_facet ZANUTTO, Marcelo de Souza
MAMIZUKA, Elza Masae
RAIZ-JÚNIOR, Roberto
LIMA, Thais Martins de
DIOGO, Constância Lima
OKAY, Thelma Suely
HAGIWARA, Mitika Kuribayashi
author_role author
author2 MAMIZUKA, Elza Masae
RAIZ-JÚNIOR, Roberto
LIMA, Thais Martins de
DIOGO, Constância Lima
OKAY, Thelma Suely
HAGIWARA, Mitika Kuribayashi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv ZANUTTO, Marcelo de Souza
MAMIZUKA, Elza Masae
RAIZ-JÚNIOR, Roberto
LIMA, Thais Martins de
DIOGO, Constância Lima
OKAY, Thelma Suely
HAGIWARA, Mitika Kuribayashi
dc.subject.por.fl_str_mv Cat
Felines
Bartonella henselae
Bartonella quintana
Hemoculture
Polymerase Chain Reaction
Indirect immunofluorescence
topic Cat
Felines
Bartonella henselae
Bartonella quintana
Hemoculture
Polymerase Chain Reaction
Indirect immunofluorescence
description In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed.
publishDate 2001
dc.date.none.fl_str_mv 2001-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30542
url https://www.revistas.usp.br/rimtsp/article/view/30542
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30542/32426
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 257-261
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 257-261
Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 257-261
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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