Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30542 |
Resumo: | In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed. |
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Experimental infection and horizontal transmission of Bartonella henselae in domestic cats Infecção experimental e transmissão horizontal de Bartonella henselae em gatos domésticos CatFelinesBartonella henselaeBartonella quintanaHemoculturePolymerase Chain ReactionIndirect immunofluorescence In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed. Procurou-se verificar a possibilidade de transmissão horizontal de B. henselae em 5 felinos confinados, dentre os quais apenas um foi inoculado experimentalmente por via subcutânea (SC) com 10(5) UFC. Todos os felinos apresentavam infestação por pulgas. Para a avaliação da infecção foram utilizados: isolamento bacteriano (hemocultura), detecção de DNA específico pela Reação em Cadeia da Polimerase (PCR), com os protocolos HSP, FTSZ e BH-PCR, e a pesquisa de anticorpos específicos por Imunofluorescência Indireta (IFI). Os protocolos da PCR foram também utilizados para a caracterização do isolado da hemocultura. A inoculação de B. henselae resultou na infecção assintomática do animal inoculado, comprovada através da soroconversão e de bacteremia de 4 meses de duração, com o isolamento da bactéria na hemocultura. Todos os animais contactantes apresentaram bacteremia 6 meses após a data de inoculação experimental. No entanto, não apresentaram reação de IFI positiva. Em nenhum momento foi possível detectar DNA de B. henselae no sangue circulante, com as PCR utilizadas. Não obstante, a PCR possibilitou a identificação da bactéria isolada como sendo do gênero Bartonella (HSP e FTSZ-PCR) e espécie henselae (BH-PCR). Conclui-se que o isolamento bacteriano por meio da hemocultura constitui-se no método mais eficiente para a identificação dos felinos infectados e bacterêmicos. Estes resultados também evidenciam a possibilidade do papel das pulgas na transmissão de B. henselae em gatos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30542Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 257-261 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 257-261 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 257-261 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30542/32426Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessZANUTTO, Marcelo de SouzaMAMIZUKA, Elza MasaeRAIZ-JÚNIOR, RobertoLIMA, Thais Martins deDIOGO, Constância LimaOKAY, Thelma SuelyHAGIWARA, Mitika Kuribayashi2012-07-07T13:35:16Zoai:revistas.usp.br:article/30542Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:19.676993Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats Infecção experimental e transmissão horizontal de Bartonella henselae em gatos domésticos |
title |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats |
spellingShingle |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats ZANUTTO, Marcelo de Souza Cat Felines Bartonella henselae Bartonella quintana Hemoculture Polymerase Chain Reaction Indirect immunofluorescence |
title_short |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats |
title_full |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats |
title_fullStr |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats |
title_full_unstemmed |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats |
title_sort |
Experimental infection and horizontal transmission of Bartonella henselae in domestic cats |
author |
ZANUTTO, Marcelo de Souza |
author_facet |
ZANUTTO, Marcelo de Souza MAMIZUKA, Elza Masae RAIZ-JÚNIOR, Roberto LIMA, Thais Martins de DIOGO, Constância Lima OKAY, Thelma Suely HAGIWARA, Mitika Kuribayashi |
author_role |
author |
author2 |
MAMIZUKA, Elza Masae RAIZ-JÚNIOR, Roberto LIMA, Thais Martins de DIOGO, Constância Lima OKAY, Thelma Suely HAGIWARA, Mitika Kuribayashi |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
ZANUTTO, Marcelo de Souza MAMIZUKA, Elza Masae RAIZ-JÚNIOR, Roberto LIMA, Thais Martins de DIOGO, Constância Lima OKAY, Thelma Suely HAGIWARA, Mitika Kuribayashi |
dc.subject.por.fl_str_mv |
Cat Felines Bartonella henselae Bartonella quintana Hemoculture Polymerase Chain Reaction Indirect immunofluorescence |
topic |
Cat Felines Bartonella henselae Bartonella quintana Hemoculture Polymerase Chain Reaction Indirect immunofluorescence |
description |
In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30542 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30542 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30542/32426 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 257-261 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 257-261 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 257-261 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951643321991168 |