Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients
Autor(a) principal: | |
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Data de Publicação: | 1995 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29297 |
Resumo: | Serum samples from 242 HIV-positive persons were studied for the detection of capsular polysaccha-ride antigen of Cryptococcus neoformans; 193 of these patients presented less than 300 CD4+ cells/µl of blood and 49 patients had more than 300 CD4+ cells/µl. None of them had symptoms or signs characteristic of cryptococcosis. The capsular antigen of C. neofarmans was detected by latex agglutination technique with pronase pre-treatment (IMMY, Crypto-Latex Antigen Detection System, Immunomycologics Inc., OK, USA); in 61% of the samples, ELISA technique was also used (Premier, Cryptococcal Antigen, Meridian Diagnostic Inc., Cincinatti, Oh, USA). The comparative study of both methods showed that the results obtained were similar in 96.9% of the cases. The capsular antigen was detected in 13 out of 193 (6.7%) patients with less than 300 CD4+ cells/µl. Cryptococcosis was confirmed mycologically in 3 of these 13 cases (23%) by the isolation of C. neoformans in CSF or blood cultures. Three patients, who had presented negative results of both tests for capsular antigen, suffered disseminated cryptococcosis 4 to 8 months later. The predictive diagnostic value of capsular antigen detection of C. neoformans seems tobe low and we believe that it should not be done routinely in asymptomatic HIV-positive persons. |
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Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients Detección del antígeno capsular del Cryptococcus neoformans en pacientes asintomáticos infectados por HIV CryptococcosisAntigenemiaLatex agglutinationELISA tests Serum samples from 242 HIV-positive persons were studied for the detection of capsular polysaccha-ride antigen of Cryptococcus neoformans; 193 of these patients presented less than 300 CD4+ cells/µl of blood and 49 patients had more than 300 CD4+ cells/µl. None of them had symptoms or signs characteristic of cryptococcosis. The capsular antigen of C. neofarmans was detected by latex agglutination technique with pronase pre-treatment (IMMY, Crypto-Latex Antigen Detection System, Immunomycologics Inc., OK, USA); in 61% of the samples, ELISA technique was also used (Premier, Cryptococcal Antigen, Meridian Diagnostic Inc., Cincinatti, Oh, USA). The comparative study of both methods showed that the results obtained were similar in 96.9% of the cases. The capsular antigen was detected in 13 out of 193 (6.7%) patients with less than 300 CD4+ cells/µl. Cryptococcosis was confirmed mycologically in 3 of these 13 cases (23%) by the isolation of C. neoformans in CSF or blood cultures. Three patients, who had presented negative results of both tests for capsular antigen, suffered disseminated cryptococcosis 4 to 8 months later. The predictive diagnostic value of capsular antigen detection of C. neoformans seems tobe low and we believe that it should not be done routinely in asymptomatic HIV-positive persons. Fueron examinadas las muestras de suero de 242 personas, HIV positivas, para determinar la presencia de antígeno capsular del C. neoformans, 193 de estos pacientes tenían recuentos de células CD4 + inferiores a los 300/µl y 49 pacientes presentaron recuentos superiores a este límite. Ninguno de los enfermos tenía sintomatología que hiciese sospechar criptococosis. El antígeno capsular del C. neoformans fue determinado por una técnica de aglutinación de partículas de látex previo tratamiento con pronasa (IMM, latex-Crypto antigen detection system, Immunomycologics, Oh, USA) y 61% de las muestras fueron también examinadas mediante la técnica de ELISA (Premier, Cryptococcal Antigen, Medirian Diagnostic Inc, Cincinatti, OH, USA). Los resultados de ambas técnicas fueron coincidentes en 96.9% de los casos. Pudo comprobarse la presencia de antígeno capsular del C. neoformans en 13 casos entre los 193 pacientes HIV positivos, con recuentos de células CD4+ menores de 300/µl (6.7%), ningún paciente con recuentos superiores a este límite presentó antigenemia positiva. En 3/13 enfermos (23%) pudo confirmarse el diagnóstico de criptococcosis mediante el aislamiento del C. neoformans en LCR o hemocultivos. Tres pacientes que habían presentado antigenemias negativas sufrieron 4 a 8 meses después, criptococosis diseminada. Se considera que el valor diagnóstico predictivo de la detección de antígeno capsular del C. neoformans es bajo y que la realización sistemática de esta reacción no se justifica. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29297Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 5 (1995); 385-389 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 5 (1995); 385-389 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 5 (1995); 385-389 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29297/31154Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessNegroni, R.Cendoya, C.Arechavala, A.I.Robles, A.M.Bianchi, M.Bava, A.J.Helou, S.2012-07-02T01:40:43Zoai:revistas.usp.br:article/29297Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:02.730833Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients Detección del antígeno capsular del Cryptococcus neoformans en pacientes asintomáticos infectados por HIV |
title |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients |
spellingShingle |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients Negroni, R. Cryptococcosis Antigenemia Latex agglutination ELISA tests |
title_short |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients |
title_full |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients |
title_fullStr |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients |
title_full_unstemmed |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients |
title_sort |
Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients |
author |
Negroni, R. |
author_facet |
Negroni, R. Cendoya, C. Arechavala, A.I. Robles, A.M. Bianchi, M. Bava, A.J. Helou, S. |
author_role |
author |
author2 |
Cendoya, C. Arechavala, A.I. Robles, A.M. Bianchi, M. Bava, A.J. Helou, S. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Negroni, R. Cendoya, C. Arechavala, A.I. Robles, A.M. Bianchi, M. Bava, A.J. Helou, S. |
dc.subject.por.fl_str_mv |
Cryptococcosis Antigenemia Latex agglutination ELISA tests |
topic |
Cryptococcosis Antigenemia Latex agglutination ELISA tests |
description |
Serum samples from 242 HIV-positive persons were studied for the detection of capsular polysaccha-ride antigen of Cryptococcus neoformans; 193 of these patients presented less than 300 CD4+ cells/µl of blood and 49 patients had more than 300 CD4+ cells/µl. None of them had symptoms or signs characteristic of cryptococcosis. The capsular antigen of C. neofarmans was detected by latex agglutination technique with pronase pre-treatment (IMMY, Crypto-Latex Antigen Detection System, Immunomycologics Inc., OK, USA); in 61% of the samples, ELISA technique was also used (Premier, Cryptococcal Antigen, Meridian Diagnostic Inc., Cincinatti, Oh, USA). The comparative study of both methods showed that the results obtained were similar in 96.9% of the cases. The capsular antigen was detected in 13 out of 193 (6.7%) patients with less than 300 CD4+ cells/µl. Cryptococcosis was confirmed mycologically in 3 of these 13 cases (23%) by the isolation of C. neoformans in CSF or blood cultures. Three patients, who had presented negative results of both tests for capsular antigen, suffered disseminated cryptococcosis 4 to 8 months later. The predictive diagnostic value of capsular antigen detection of C. neoformans seems tobe low and we believe that it should not be done routinely in asymptomatic HIV-positive persons. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29297 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29297 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29297/31154 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 5 (1995); 385-389 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 5 (1995); 385-389 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 5 (1995); 385-389 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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