Pathogenicity of different rabies virus isolates and protection test in vaccinated mice

Detalhes bibliográficos
Autor(a) principal: Cunha, Elenice M.S.
Data de Publicação: 2010
Outros Autores: Nassar, Alessandra F.C., Lara, Maria do Carmo C.S.H., Villalobos, Eliana C.M., Sato, Go, Kobayashi, Yuki, Shoji, Youko, Itou, Takuya, Sakai, Takeo, Ito, Fumio H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31343
Resumo: This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate.
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spelling Pathogenicity of different rabies virus isolates and protection test in vaccinated mice Patogenicidade de diferentes isolados do vírus da raiva e teste de proteção em camundongos vacinados Rabies virusPathogenicityVaccine This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate. O estudo avaliou e comparou as propriedades patogênicas de cinco isolados do vírus da raiva de morcegos e um isolado do vírus da raiva de cão e analisou a eficácia de vacina comercial contra estes isolados, em camundongos. Para o estudo de patogenicidade camundongos foram inoculados pela via IM com 0,1 mL contendo 500MICLD50/0,03mL das amostras de vírus. Quando inoculados pela via IC, os isolados do vírus da raiva provocaram a morte de 100% dos camundongos. No entanto, 500MICLD50/0,03mL das mesmas amostras, inoculadas pela via IM, ocasionaram mortalidade de: 60,0% quando a amostra era de Desmodus rotundus; 50,0% de cão e de Nyctinomops laticaudatus; 40,0% de Artibeus lituratus; 9,5% de Molossus molossus; e 5,2% de Eptesicus furinalis. Camundongos que receberam duas doses de vacina foram protegidos quando desafiados pela via IC, com todas as amostras testadas. Quando os camundongos receberam uma dose da mesma vacina, houve proteção parcial daqueles desafiados com a amostra de cão. Todos os isolados do vírus da raiva testados foram patogênicos para camundongos, inoculados pela IC. No entanto, pela via IM, os mesmos isolados mostraram diferentes graus de patogenicidade. Concluiu-se também que a vacina comercial contra raiva protegeu os camundongos desafiados com amostras de vírus isolados de morcegos e de cão. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31343Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 5 (2010); 231-236 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 5 (2010); 231-236 Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 5 (2010); 231-236 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31343/33228Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCunha, Elenice M.S.Nassar, Alessandra F.C.Lara, Maria do Carmo C.S.H.Villalobos, Eliana C.M.Sato, GoKobayashi, YukiShoji, YoukoItou, TakuyaSakai, TakeoIto, Fumio H.2012-07-07T19:32:29Zoai:revistas.usp.br:article/31343Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:01.070477Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
Patogenicidade de diferentes isolados do vírus da raiva e teste de proteção em camundongos vacinados
title Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
spellingShingle Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
Cunha, Elenice M.S.
Rabies virus
Pathogenicity
Vaccine
title_short Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
title_full Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
title_fullStr Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
title_full_unstemmed Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
title_sort Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
author Cunha, Elenice M.S.
author_facet Cunha, Elenice M.S.
Nassar, Alessandra F.C.
Lara, Maria do Carmo C.S.H.
Villalobos, Eliana C.M.
Sato, Go
Kobayashi, Yuki
Shoji, Youko
Itou, Takuya
Sakai, Takeo
Ito, Fumio H.
author_role author
author2 Nassar, Alessandra F.C.
Lara, Maria do Carmo C.S.H.
Villalobos, Eliana C.M.
Sato, Go
Kobayashi, Yuki
Shoji, Youko
Itou, Takuya
Sakai, Takeo
Ito, Fumio H.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Elenice M.S.
Nassar, Alessandra F.C.
Lara, Maria do Carmo C.S.H.
Villalobos, Eliana C.M.
Sato, Go
Kobayashi, Yuki
Shoji, Youko
Itou, Takuya
Sakai, Takeo
Ito, Fumio H.
dc.subject.por.fl_str_mv Rabies virus
Pathogenicity
Vaccine
topic Rabies virus
Pathogenicity
Vaccine
description This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31343
url https://www.revistas.usp.br/rimtsp/article/view/31343
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31343/33228
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 5 (2010); 231-236
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 5 (2010); 231-236
Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 5 (2010); 231-236
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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