Pathogenicity of different rabies virus isolates and protection test in vaccinated mice
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31343 |
Resumo: | This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate. |
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Pathogenicity of different rabies virus isolates and protection test in vaccinated mice Patogenicidade de diferentes isolados do vírus da raiva e teste de proteção em camundongos vacinados Rabies virusPathogenicityVaccine This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate. O estudo avaliou e comparou as propriedades patogênicas de cinco isolados do vírus da raiva de morcegos e um isolado do vírus da raiva de cão e analisou a eficácia de vacina comercial contra estes isolados, em camundongos. Para o estudo de patogenicidade camundongos foram inoculados pela via IM com 0,1 mL contendo 500MICLD50/0,03mL das amostras de vírus. Quando inoculados pela via IC, os isolados do vírus da raiva provocaram a morte de 100% dos camundongos. No entanto, 500MICLD50/0,03mL das mesmas amostras, inoculadas pela via IM, ocasionaram mortalidade de: 60,0% quando a amostra era de Desmodus rotundus; 50,0% de cão e de Nyctinomops laticaudatus; 40,0% de Artibeus lituratus; 9,5% de Molossus molossus; e 5,2% de Eptesicus furinalis. Camundongos que receberam duas doses de vacina foram protegidos quando desafiados pela via IC, com todas as amostras testadas. Quando os camundongos receberam uma dose da mesma vacina, houve proteção parcial daqueles desafiados com a amostra de cão. Todos os isolados do vírus da raiva testados foram patogênicos para camundongos, inoculados pela IC. No entanto, pela via IM, os mesmos isolados mostraram diferentes graus de patogenicidade. Concluiu-se também que a vacina comercial contra raiva protegeu os camundongos desafiados com amostras de vírus isolados de morcegos e de cão. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31343Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 5 (2010); 231-236 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 5 (2010); 231-236 Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 5 (2010); 231-236 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31343/33228Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCunha, Elenice M.S.Nassar, Alessandra F.C.Lara, Maria do Carmo C.S.H.Villalobos, Eliana C.M.Sato, GoKobayashi, YukiShoji, YoukoItou, TakuyaSakai, TakeoIto, Fumio H.2012-07-07T19:32:29Zoai:revistas.usp.br:article/31343Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:01.070477Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice Patogenicidade de diferentes isolados do vírus da raiva e teste de proteção em camundongos vacinados |
title |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice |
spellingShingle |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice Cunha, Elenice M.S. Rabies virus Pathogenicity Vaccine |
title_short |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice |
title_full |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice |
title_fullStr |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice |
title_full_unstemmed |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice |
title_sort |
Pathogenicity of different rabies virus isolates and protection test in vaccinated mice |
author |
Cunha, Elenice M.S. |
author_facet |
Cunha, Elenice M.S. Nassar, Alessandra F.C. Lara, Maria do Carmo C.S.H. Villalobos, Eliana C.M. Sato, Go Kobayashi, Yuki Shoji, Youko Itou, Takuya Sakai, Takeo Ito, Fumio H. |
author_role |
author |
author2 |
Nassar, Alessandra F.C. Lara, Maria do Carmo C.S.H. Villalobos, Eliana C.M. Sato, Go Kobayashi, Yuki Shoji, Youko Itou, Takuya Sakai, Takeo Ito, Fumio H. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Elenice M.S. Nassar, Alessandra F.C. Lara, Maria do Carmo C.S.H. Villalobos, Eliana C.M. Sato, Go Kobayashi, Yuki Shoji, Youko Itou, Takuya Sakai, Takeo Ito, Fumio H. |
dc.subject.por.fl_str_mv |
Rabies virus Pathogenicity Vaccine |
topic |
Rabies virus Pathogenicity Vaccine |
description |
This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31343 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31343/33228 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 5 (2010); 231-236 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 5 (2010); 231-236 Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 5 (2010); 231-236 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951647863373824 |