Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica

Detalhes bibliográficos
Autor(a) principal: Gomes, Maria Aparecida
Data de Publicação: 1995
Outros Autores: Martins, Maria Sonia, Costa, Adriana Oliveira, Silva, Edward Félix
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29261
Resumo: At this moment, the duality of species suggested for E. histolytica is being considered for discussion. In order to contribute to settling this question, we investigated the possibility of conversion of avirulent ameba to virulent ones, as well as, the possibility of increasing virulence of virulent strains, by means of association with bacteria. Five strains of E. histolytica were employed, two of them regarded as avirulent and three virulent ones. Amebas were associated with the bacteria Escherichia coli 055 and 0115, previously demonstrated as capable to modify the pathogenic behavior of E. histolytica. Changes in virulence of amebas were assessed by cytopathic effect upon cultured mammal cells and erythrophagocytosis. The virulence of pathogenic strains was significantly increased after bacteria association in opposition to what was observed for nonpathogenic ones, which were not influenced by bacteria association.
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spelling Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica Influência de bacterias sobre o efeito citopático e a eritrofagocitose de diferentes espécies axênicas de Entamoeba histolytica Ameba-bacteria interactionCytopathic effectErythrophagocytosisVirulence At this moment, the duality of species suggested for E. histolytica is being considered for discussion. In order to contribute to settling this question, we investigated the possibility of conversion of avirulent ameba to virulent ones, as well as, the possibility of increasing virulence of virulent strains, by means of association with bacteria. Five strains of E. histolytica were employed, two of them regarded as avirulent and three virulent ones. Amebas were associated with the bacteria Escherichia coli 055 and 0115, previously demonstrated as capable to modify the pathogenic behavior of E. histolytica. Changes in virulence of amebas were assessed by cytopathic effect upon cultured mammal cells and erythrophagocytosis. The virulence of pathogenic strains was significantly increased after bacteria association in opposition to what was observed for nonpathogenic ones, which were not influenced by bacteria association. Atualmente, tem se discutido muito a dualidade da Entamoeba histolytica. Na tentativa de contribuir no esclarecimento desta questão, investigamos a possibilidade de conversão de amebas avirulentas em virulentas, como também a possibilidade de aumento de virulência de cepas virulentas seguido da associação com bactérias. Para tal utilizamos 5 cepas de E. histolytica, 2 avirulentas e 3 virulentas. As amebas foram associadas com as bactérias Escherichia coli 055 e 0115, demonstradas previamente como hábeis para modificar o comportamento patogênico da E. histolytica. As modificações na virulência das amebas foram avaliadas através do efeito citopático sobre células de mamífero em cultivo e eritrofagocitose. A virulência das cepas patogênicas foi significantemente aumentada depois da associação com bactérias em oposto ao observado para as cepas não patogênicas, as quais não foram influenciadas pela associação com bactérias. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29261Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 3 (1995); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 3 (1995); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 3 (1995); 197-200 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29261/31118Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGomes, Maria AparecidaMartins, Maria SoniaCosta, Adriana OliveiraSilva, Edward Félix2012-07-02T01:40:05Zoai:revistas.usp.br:article/29261Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:00.673109Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
Influência de bacterias sobre o efeito citopático e a eritrofagocitose de diferentes espécies axênicas de Entamoeba histolytica
title Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
spellingShingle Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
Gomes, Maria Aparecida
Ameba-bacteria interaction
Cytopathic effect
Erythrophagocytosis
Virulence
title_short Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
title_full Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
title_fullStr Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
title_full_unstemmed Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
title_sort Influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of Entamoeba histolytica
author Gomes, Maria Aparecida
author_facet Gomes, Maria Aparecida
Martins, Maria Sonia
Costa, Adriana Oliveira
Silva, Edward Félix
author_role author
author2 Martins, Maria Sonia
Costa, Adriana Oliveira
Silva, Edward Félix
author2_role author
author
author
dc.contributor.author.fl_str_mv Gomes, Maria Aparecida
Martins, Maria Sonia
Costa, Adriana Oliveira
Silva, Edward Félix
dc.subject.por.fl_str_mv Ameba-bacteria interaction
Cytopathic effect
Erythrophagocytosis
Virulence
topic Ameba-bacteria interaction
Cytopathic effect
Erythrophagocytosis
Virulence
description At this moment, the duality of species suggested for E. histolytica is being considered for discussion. In order to contribute to settling this question, we investigated the possibility of conversion of avirulent ameba to virulent ones, as well as, the possibility of increasing virulence of virulent strains, by means of association with bacteria. Five strains of E. histolytica were employed, two of them regarded as avirulent and three virulent ones. Amebas were associated with the bacteria Escherichia coli 055 and 0115, previously demonstrated as capable to modify the pathogenic behavior of E. histolytica. Changes in virulence of amebas were assessed by cytopathic effect upon cultured mammal cells and erythrophagocytosis. The virulence of pathogenic strains was significantly increased after bacteria association in opposition to what was observed for nonpathogenic ones, which were not influenced by bacteria association.
publishDate 1995
dc.date.none.fl_str_mv 1995-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29261
url https://www.revistas.usp.br/rimtsp/article/view/29261
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29261/31118
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 3 (1995); 197-200
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 3 (1995); 197-200
Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 3 (1995); 197-200
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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