Entamoeba histolytica: antigenic characterization of axenic strains from Brazil

Detalhes bibliográficos
Autor(a) principal: Guimarães, Semíramis
Data de Publicação: 1991
Outros Autores: Urdaneta, Haidée, Silva, Edward F., Tavares, Carlos Alberto P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28784
Resumo: Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.
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spelling Entamoeba histolytica: antigenic characterization of axenic strains from Brazil Entamoeba histolytica: caracterização antigênica de cepas axênicas do Brasil Entamoeba histolyticaAxenic strainsAntigens Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains. Trofozoítos obtidos de cultura de Entamoeba histolytica isoladas e axenizadas no Brasil (ICB-CSP, ICB-462 e ICB-32) foram utilizados para a produção de soros imunes em coelhos e para a caracterização de antígenos através dos seus perfis eletroforético e glicoproteico, em paralelo com uma cepa padrão isolada e axenizada nos Estados Unidos (HK-9). Obtiveram-se soros hiperimunes, reativos frente aos antígenos homólogos e heterólogos. As quatro cepas em estudo apresentaram perfis eletroforético e glicoproteico complexos e semelhantes, compostos por polipeptídeos com pesos moleculares variando de 200 kDa a menos de 29 kDa. Não se detectaram diferenças significativas entre as cepas patogênicas e não patogênicas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28784Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 1 (1991); 6-11 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 1 (1991); 6-11 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 1 (1991); 6-11 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28784/30637Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGuimarães, SemíramisUrdaneta, HaidéeSilva, Edward F.Tavares, Carlos Alberto P.2012-07-02T01:29:39Zoai:revistas.usp.br:article/28784Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:32.742862Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
Entamoeba histolytica: caracterização antigênica de cepas axênicas do Brasil
title Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
spellingShingle Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
Guimarães, Semíramis
Entamoeba histolytica
Axenic strains
Antigens
title_short Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
title_full Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
title_fullStr Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
title_full_unstemmed Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
title_sort Entamoeba histolytica: antigenic characterization of axenic strains from Brazil
author Guimarães, Semíramis
author_facet Guimarães, Semíramis
Urdaneta, Haidée
Silva, Edward F.
Tavares, Carlos Alberto P.
author_role author
author2 Urdaneta, Haidée
Silva, Edward F.
Tavares, Carlos Alberto P.
author2_role author
author
author
dc.contributor.author.fl_str_mv Guimarães, Semíramis
Urdaneta, Haidée
Silva, Edward F.
Tavares, Carlos Alberto P.
dc.subject.por.fl_str_mv Entamoeba histolytica
Axenic strains
Antigens
topic Entamoeba histolytica
Axenic strains
Antigens
description Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.
publishDate 1991
dc.date.none.fl_str_mv 1991-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28784
url https://www.revistas.usp.br/rimtsp/article/view/28784
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28784/30637
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 1 (1991); 6-11
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 1 (1991); 6-11
Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 1 (1991); 6-11
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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