Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes

Detalhes bibliográficos
Autor(a) principal: Milagres, Lucimar Gonçalves
Data de Publicação: 1992
Outros Autores: Melles, Carmo E.A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28939
Resumo: S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction.
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spelling Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos Staphylococcus saprophyticusAderênciaCélulas HEp-2Hemaglutinação S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction. S. saprophyticus é freqüentemente isolado de infecções do trato urinário de mulheres jovens e sexualmente ativas. Ao contrário de S. aureus, esta espécie não possui fatores de virulência bem definidos. O objetivo deste estudo é analisar a aderência de S. saprophyticus a células HEp-2 e eritrócitos de carneiro. As amostras foram isoladas a partir da urina de pacientes com infecção urinária. Foram realizados testes de hemaglutinação, aderência a células HEp-2 e a capacidade de carboidratos específicos inibirem as interações entre estes tipos celulares e S. saprophyticus. A maioria das cepas se mostrou hemaglutinante e sensível a inibição da hemaglutinação pela manose (100mM). Foram verificados altos níveis de aderência às células HEp-2. As diferenças em especificidade e nível de aderência do microrganismo a células de HEp-2 e eritrócitos sugerem a participação de diferentes adesinas nos processos de interações celulares. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28939Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 4 (1992); 315-321 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 4 (1992); 315-321 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 4 (1992); 315-321 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28939/30796Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMilagres, Lucimar GonçalvesMelles, Carmo E.A.2012-07-02T01:33:16Zoai:revistas.usp.br:article/28939Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:42.296122Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos
title Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
spellingShingle Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
Milagres, Lucimar Gonçalves
Staphylococcus saprophyticus
Aderência
Células HEp-2
Hemaglutinação
title_short Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
title_full Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
title_fullStr Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
title_full_unstemmed Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
title_sort Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
author Milagres, Lucimar Gonçalves
author_facet Milagres, Lucimar Gonçalves
Melles, Carmo E.A.
author_role author
author2 Melles, Carmo E.A.
author2_role author
dc.contributor.author.fl_str_mv Milagres, Lucimar Gonçalves
Melles, Carmo E.A.
dc.subject.por.fl_str_mv Staphylococcus saprophyticus
Aderência
Células HEp-2
Hemaglutinação
topic Staphylococcus saprophyticus
Aderência
Células HEp-2
Hemaglutinação
description S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction.
publishDate 1992
dc.date.none.fl_str_mv 1992-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28939
url https://www.revistas.usp.br/rimtsp/article/view/28939
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28939/30796
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 4 (1992); 315-321
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 4 (1992); 315-321
Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 4 (1992); 315-321
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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