Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28939 |
Resumo: | S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction. |
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Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos Staphylococcus saprophyticusAderênciaCélulas HEp-2Hemaglutinação S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction. S. saprophyticus é freqüentemente isolado de infecções do trato urinário de mulheres jovens e sexualmente ativas. Ao contrário de S. aureus, esta espécie não possui fatores de virulência bem definidos. O objetivo deste estudo é analisar a aderência de S. saprophyticus a células HEp-2 e eritrócitos de carneiro. As amostras foram isoladas a partir da urina de pacientes com infecção urinária. Foram realizados testes de hemaglutinação, aderência a células HEp-2 e a capacidade de carboidratos específicos inibirem as interações entre estes tipos celulares e S. saprophyticus. A maioria das cepas se mostrou hemaglutinante e sensível a inibição da hemaglutinação pela manose (100mM). Foram verificados altos níveis de aderência às células HEp-2. As diferenças em especificidade e nível de aderência do microrganismo a células de HEp-2 e eritrócitos sugerem a participação de diferentes adesinas nos processos de interações celulares. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28939Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 4 (1992); 315-321 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 4 (1992); 315-321 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 4 (1992); 315-321 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28939/30796Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMilagres, Lucimar GonçalvesMelles, Carmo E.A.2012-07-02T01:33:16Zoai:revistas.usp.br:article/28939Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:42.296122Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos |
title |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes |
spellingShingle |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes Milagres, Lucimar Gonçalves Staphylococcus saprophyticus Aderência Células HEp-2 Hemaglutinação |
title_short |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes |
title_full |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes |
title_fullStr |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes |
title_full_unstemmed |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes |
title_sort |
Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes |
author |
Milagres, Lucimar Gonçalves |
author_facet |
Milagres, Lucimar Gonçalves Melles, Carmo E.A. |
author_role |
author |
author2 |
Melles, Carmo E.A. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Milagres, Lucimar Gonçalves Melles, Carmo E.A. |
dc.subject.por.fl_str_mv |
Staphylococcus saprophyticus Aderência Células HEp-2 Hemaglutinação |
topic |
Staphylococcus saprophyticus Aderência Células HEp-2 Hemaglutinação |
description |
S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28939 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28939 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28939/30796 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 4 (1992); 315-321 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 4 (1992); 315-321 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 4 (1992); 315-321 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951639466377216 |