A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy
Autor(a) principal: | |
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Data de Publicação: | 1986 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/87470 |
Resumo: | In most patients with chronic Chagas disease the Trypanosoma cruzi has been shown to be present in extremely scanty numbers in all tissues extensively studied using conventional non-specific histologic techniques presently available. The present work was carried out in order to compare the immunocytochemical method of peroxidase anti-peroxidase (PAP) with the Haematoxilin and Eosin (HE) technique in the quantification of the parasitism in formalin fixed tissues from chronic chagasic patients. Using the same tissue fragment from each one of 3 cases of chronic chagasic cardiomyopathy, sections were obtained for HE (average: 100 sections per fragment) and for PAP (average: 70 sections per fragment). The fragment from case Nip. 1 was first studied in 1952 and presented with relatively great number of parasites. The results, expressed as amastigotes nests per 100 sections stained by HE and PAP were, respectively (HE PAP): case No. 1, 80 171; case No. 2, 5-116 and case No. 3, 1-2. The present results show that the immunocytochemical method improves the quantitative evaluation of the parasitism in tissues of chronic Chagas disease. Furthemore. it should be preferred not only for the specific diagnosis taut also for quantitative studies on the tissular parasitism even in those tissues included in paraffin wax for long periods of time. |
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A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy Estudo comparativo entre o método convencional e o método da peroxidase anti-peroxidase na pesquisa do parasitismo tissular na cardiopatia chagásica crônica In most patients with chronic Chagas disease the Trypanosoma cruzi has been shown to be present in extremely scanty numbers in all tissues extensively studied using conventional non-specific histologic techniques presently available. The present work was carried out in order to compare the immunocytochemical method of peroxidase anti-peroxidase (PAP) with the Haematoxilin and Eosin (HE) technique in the quantification of the parasitism in formalin fixed tissues from chronic chagasic patients. Using the same tissue fragment from each one of 3 cases of chronic chagasic cardiomyopathy, sections were obtained for HE (average: 100 sections per fragment) and for PAP (average: 70 sections per fragment). The fragment from case Nip. 1 was first studied in 1952 and presented with relatively great number of parasites. The results, expressed as amastigotes nests per 100 sections stained by HE and PAP were, respectively (HE PAP): case No. 1, 80 171; case No. 2, 5-116 and case No. 3, 1-2. The present results show that the immunocytochemical method improves the quantitative evaluation of the parasitism in tissues of chronic Chagas disease. Furthemore. it should be preferred not only for the specific diagnosis taut also for quantitative studies on the tissular parasitism even in those tissues included in paraffin wax for long periods of time. Na maioria dos chagásicos crônicos o Trypanosoma cruzi não é detectado no tecido ou apresenta se com extrema raridade, mesmo quando é pesquisado exaustivamente. Sendo os métodos utilizados, até então, inespecíficos para a demonstração do T. cruzi, propôs-se no presente trabalho proceder ao estudo comparativo entre o método convencional (HE) e o método imunocitoquímico pela peroxidase anti-peroxidase (PAP), na avaliação quantitativa do parasitismo. Selecionaram-se 3 casos de cardiopatia chagásica crônica e, de um mesmo fragmento de cada caso, obtiveram se cortes que foram corados pelo H.E. (média de 100 cortes por caso) e, consecutivamente, outros que foram corados pelo PAP (média de 70 cortes por caso). O caso n.° 1 foi autopsiado em 1952 e apresentava parasitismo freqüente. Nos demais, o exame rotineiro foi negativo. Obtiveram-se os seguintes resultados expressos em n.° de ninhos/100 cortes, respectivamente, corados pelo H.E. e pelo P.A.P. (HE/PAP). Caso n.° 1 - 80/171; caso n.° 2 = 5/116 e caso n.° 3 = 1/2. Os resultados mostram que o método imunocitoquímico empregado, além de facilitar o diagnóstico do parasitismo, demonstra também pequenos ninhos de amastigotas que dificilmente seriam diagnosticados pelos métodos convencionais; além disso mostrou-se útil mesmo em tecido incluídos em parafina há longo tempo. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1986-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/87470Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 No. 2 (1986); 91-96Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 Núm. 2 (1986); 91-96Revista do Instituto de Medicina Tropical de São Paulo; v. 28 n. 2 (1986); 91-961678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/87470/90428Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessBarbosa, A. J. A.Gobbi, H.Lino, B. T.Lages-Silva, E.Ramirez, L. E.Teixeira, V. P. A.Almeida, H. O.2014-11-07T18:33:33Zoai:revistas.usp.br:article/87470Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:26.305769Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy Estudo comparativo entre o método convencional e o método da peroxidase anti-peroxidase na pesquisa do parasitismo tissular na cardiopatia chagásica crônica |
title |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy |
spellingShingle |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy Barbosa, A. J. A. |
title_short |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy |
title_full |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy |
title_fullStr |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy |
title_full_unstemmed |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy |
title_sort |
A comparative study between Haematoxilin and Eosin and the peroxidase anti-peroxidase stains for tissular parasitism quantification in chronic chagasic cardiomyopathy |
author |
Barbosa, A. J. A. |
author_facet |
Barbosa, A. J. A. Gobbi, H. Lino, B. T. Lages-Silva, E. Ramirez, L. E. Teixeira, V. P. A. Almeida, H. O. |
author_role |
author |
author2 |
Gobbi, H. Lino, B. T. Lages-Silva, E. Ramirez, L. E. Teixeira, V. P. A. Almeida, H. O. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Barbosa, A. J. A. Gobbi, H. Lino, B. T. Lages-Silva, E. Ramirez, L. E. Teixeira, V. P. A. Almeida, H. O. |
description |
In most patients with chronic Chagas disease the Trypanosoma cruzi has been shown to be present in extremely scanty numbers in all tissues extensively studied using conventional non-specific histologic techniques presently available. The present work was carried out in order to compare the immunocytochemical method of peroxidase anti-peroxidase (PAP) with the Haematoxilin and Eosin (HE) technique in the quantification of the parasitism in formalin fixed tissues from chronic chagasic patients. Using the same tissue fragment from each one of 3 cases of chronic chagasic cardiomyopathy, sections were obtained for HE (average: 100 sections per fragment) and for PAP (average: 70 sections per fragment). The fragment from case Nip. 1 was first studied in 1952 and presented with relatively great number of parasites. The results, expressed as amastigotes nests per 100 sections stained by HE and PAP were, respectively (HE PAP): case No. 1, 80 171; case No. 2, 5-116 and case No. 3, 1-2. The present results show that the immunocytochemical method improves the quantitative evaluation of the parasitism in tissues of chronic Chagas disease. Furthemore. it should be preferred not only for the specific diagnosis taut also for quantitative studies on the tissular parasitism even in those tissues included in paraffin wax for long periods of time. |
publishDate |
1986 |
dc.date.none.fl_str_mv |
1986-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/87470 |
url |
https://www.revistas.usp.br/rimtsp/article/view/87470 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/87470/90428 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 No. 2 (1986); 91-96 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 Núm. 2 (1986); 91-96 Revista do Instituto de Medicina Tropical de São Paulo; v. 28 n. 2 (1986); 91-96 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951650173386752 |