Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/162393 |
Resumo: | Several arboviruses have emerged and/or re-emerged in North, Central and SouthAmerican countries. Viruses from some regions of Africa and Asia, such as the Zika and Chikungunya virus have been introduced in new continents causing major public health problems. The aim of this study was to investigate the presence of RNA from Zika, Dengue and Chikungunya viruses in symptomatic patients from Rondonia, where the epidemiological profile is still little known, by one-step real-time RT-PCR. The main clinical signs and symtoms were fever (51.2%), headache (78%), chills (6.1%), pruritus (12.2%), exanthema (20.1%), arthralgia (35.3%), myalgia (26.8%) and retro-orbital pain (19.5%). Serum from 164 symptomatic patients were collected and tested for RNA of Zika, Dengue types 1 to 4 and Chikungunya viruses, in addition to antibodies against Dengue NS1 antigen. Direct microscopy for Malaria was also performed. Only ZIKV RNA was detected in 4.3% of the patients, and in the remaining 95.7% of the patients RNA for Zika, Dengue and Chikungunya viruses were not detected. This finding is intriguing as the region has been endemic for Dengue for a long time and more recently for Chikungunya virus as well. The results indicated that medical and molecular parameters obtained were suitable to describe the first report of symptomatic Zika infections in this region. Furthermore, the low rate of detection, compared to clinical signs and symptoms as the solely diagnosis criteria, suggests that molecular assays for detection of viruses or other pathogens that cause similar symptoms should be used and the corresponding diseases could be included in the compulsory notification list. |
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Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, BrazilZikaChikungunyaDengueOne-step real-time RT-PCRDiagnosticArbovirusesSeveral arboviruses have emerged and/or re-emerged in North, Central and SouthAmerican countries. Viruses from some regions of Africa and Asia, such as the Zika and Chikungunya virus have been introduced in new continents causing major public health problems. The aim of this study was to investigate the presence of RNA from Zika, Dengue and Chikungunya viruses in symptomatic patients from Rondonia, where the epidemiological profile is still little known, by one-step real-time RT-PCR. The main clinical signs and symtoms were fever (51.2%), headache (78%), chills (6.1%), pruritus (12.2%), exanthema (20.1%), arthralgia (35.3%), myalgia (26.8%) and retro-orbital pain (19.5%). Serum from 164 symptomatic patients were collected and tested for RNA of Zika, Dengue types 1 to 4 and Chikungunya viruses, in addition to antibodies against Dengue NS1 antigen. Direct microscopy for Malaria was also performed. Only ZIKV RNA was detected in 4.3% of the patients, and in the remaining 95.7% of the patients RNA for Zika, Dengue and Chikungunya viruses were not detected. This finding is intriguing as the region has been endemic for Dengue for a long time and more recently for Chikungunya virus as well. The results indicated that medical and molecular parameters obtained were suitable to describe the first report of symptomatic Zika infections in this region. Furthermore, the low rate of detection, compared to clinical signs and symptoms as the solely diagnosis criteria, suggests that molecular assays for detection of viruses or other pathogens that cause similar symptoms should be used and the corresponding diseases could be included in the compulsory notification list.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2019-09-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/16239310.1590/s1678-9946201961040Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e40Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e40Revista do Instituto de Medicina Tropical de São Paulo; v. 61 (2019); e401678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/162393/156184https://www.revistas.usp.br/rimtsp/article/view/162393/156185Copyright (c) 2019 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVieira, Deusilene SouzaZambenedetti, Miriam RibasRequião, LucianaBorghetti, Ivo AlbertoLuna, Luciano Kleber de SouzaSantos, Alcione de Oliveira dosTaborda, Roger Lafontaine MesquitaPereira, Dhelio BatistaKrieger, Marco AurélioSalcedo, Juan Miguel VillalobosRampazzo, Rita de Cássia Pontello2019-09-18T14:54:30Zoai:revistas.usp.br:article/162393Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:50.051363Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
title |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
spellingShingle |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil Vieira, Deusilene Souza Zika Chikungunya Dengue One-step real-time RT-PCR Diagnostic Arboviruses |
title_short |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
title_full |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
title_fullStr |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
title_full_unstemmed |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
title_sort |
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil |
author |
Vieira, Deusilene Souza |
author_facet |
Vieira, Deusilene Souza Zambenedetti, Miriam Ribas Requião, Luciana Borghetti, Ivo Alberto Luna, Luciano Kleber de Souza Santos, Alcione de Oliveira dos Taborda, Roger Lafontaine Mesquita Pereira, Dhelio Batista Krieger, Marco Aurélio Salcedo, Juan Miguel Villalobos Rampazzo, Rita de Cássia Pontello |
author_role |
author |
author2 |
Zambenedetti, Miriam Ribas Requião, Luciana Borghetti, Ivo Alberto Luna, Luciano Kleber de Souza Santos, Alcione de Oliveira dos Taborda, Roger Lafontaine Mesquita Pereira, Dhelio Batista Krieger, Marco Aurélio Salcedo, Juan Miguel Villalobos Rampazzo, Rita de Cássia Pontello |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Deusilene Souza Zambenedetti, Miriam Ribas Requião, Luciana Borghetti, Ivo Alberto Luna, Luciano Kleber de Souza Santos, Alcione de Oliveira dos Taborda, Roger Lafontaine Mesquita Pereira, Dhelio Batista Krieger, Marco Aurélio Salcedo, Juan Miguel Villalobos Rampazzo, Rita de Cássia Pontello |
dc.subject.por.fl_str_mv |
Zika Chikungunya Dengue One-step real-time RT-PCR Diagnostic Arboviruses |
topic |
Zika Chikungunya Dengue One-step real-time RT-PCR Diagnostic Arboviruses |
description |
Several arboviruses have emerged and/or re-emerged in North, Central and SouthAmerican countries. Viruses from some regions of Africa and Asia, such as the Zika and Chikungunya virus have been introduced in new continents causing major public health problems. The aim of this study was to investigate the presence of RNA from Zika, Dengue and Chikungunya viruses in symptomatic patients from Rondonia, where the epidemiological profile is still little known, by one-step real-time RT-PCR. The main clinical signs and symtoms were fever (51.2%), headache (78%), chills (6.1%), pruritus (12.2%), exanthema (20.1%), arthralgia (35.3%), myalgia (26.8%) and retro-orbital pain (19.5%). Serum from 164 symptomatic patients were collected and tested for RNA of Zika, Dengue types 1 to 4 and Chikungunya viruses, in addition to antibodies against Dengue NS1 antigen. Direct microscopy for Malaria was also performed. Only ZIKV RNA was detected in 4.3% of the patients, and in the remaining 95.7% of the patients RNA for Zika, Dengue and Chikungunya viruses were not detected. This finding is intriguing as the region has been endemic for Dengue for a long time and more recently for Chikungunya virus as well. The results indicated that medical and molecular parameters obtained were suitable to describe the first report of symptomatic Zika infections in this region. Furthermore, the low rate of detection, compared to clinical signs and symptoms as the solely diagnosis criteria, suggests that molecular assays for detection of viruses or other pathogens that cause similar symptoms should be used and the corresponding diseases could be included in the compulsory notification list. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/162393 10.1590/s1678-9946201961040 |
url |
https://www.revistas.usp.br/rimtsp/article/view/162393 |
identifier_str_mv |
10.1590/s1678-9946201961040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/162393/156184 https://www.revistas.usp.br/rimtsp/article/view/162393/156185 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e40 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e40 Revista do Instituto de Medicina Tropical de São Paulo; v. 61 (2019); e40 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
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Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
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IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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