Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus

Detalhes bibliográficos
Autor(a) principal: Rezende, Nilton Alves de
Data de Publicação: 1995
Outros Autores: Dias, Mariana Borges, Campolina, Délio, Chavéz-Olortegui, Carlos, Amaral, Carlos Faria Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29233
Resumo: The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified as mild cases and 19 as moderate or severe cases. The control absorbance in the venom assay was provided by serum samples from 100 individuals of same socioeconomic group and geographical area who had never been stung by scorpions or treated with horse antisera. The negative cutoff value (mean + 2 SD) corresponded to a venom concentration of 4.8 ng/ml. Three out of the 100 normal sera were positive, resulting in a specificity of 97%. The sensitivity of the ELISA when all cases of scorpion sting were included was 39.3%. When mild cases were excluded, the sensitivity increased to 94.7%. This study showed that this ELISA can be used for the detection of circulating venom toxic antigens in patients with systemic manifestations following. T. serrulatus sting but cannot be used for clinical studies in mild cases of envenoming since the test does not discriminate mild cases from control patients.
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spelling Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus ImmunodiagnosisScorpion envenoming The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified as mild cases and 19 as moderate or severe cases. The control absorbance in the venom assay was provided by serum samples from 100 individuals of same socioeconomic group and geographical area who had never been stung by scorpions or treated with horse antisera. The negative cutoff value (mean + 2 SD) corresponded to a venom concentration of 4.8 ng/ml. Three out of the 100 normal sera were positive, resulting in a specificity of 97%. The sensitivity of the ELISA when all cases of scorpion sting were included was 39.3%. When mild cases were excluded, the sensitivity increased to 94.7%. This study showed that this ELISA can be used for the detection of circulating venom toxic antigens in patients with systemic manifestations following. T. serrulatus sting but cannot be used for clinical studies in mild cases of envenoming since the test does not discriminate mild cases from control patients. Neste trabalho foram determinadas a sensibilidade e a especificidade da técnica imunoenzimática (ELISA) desenvolvida por CHAVÉZ-OLORTEGUI et al. para detectar antígenos circulantes de veneno em pacientes picados po Tityus serrulatus. A média mais dois desvios padrão da observância do soro de 100 pacientes controles foi utilizada como limite entre teste positivo e teste negativo ("cutoff"). A especificidade do ELISA foi igual a 97,0%. A sensibilidade do método, quando incluidos pacientes classificados como casos leves, moderados e graves de escorpionismo, foi de 39,3% e aumentou para 94,7% quando considerados apenas os casos moderados e graves. Estes resultados mostram que o ELISA pode ser utilizado para detecção de antígenos tóxicos circulantes em pacientes com manifestações sistêmicas de envenenamento escorpiônico mas não deve ser empregado no estudo de pacientes que apresentam apenas dor no local da picada (casos leves). O tempo necessário para a realização do ELISA é superior a 1 hora. Portanto, o teste tem sua utilização limitada para o diagnóstico de envenenamento, mas pode constituir um instrumento útil para o estudo da cinética de neutralização do veneno pelo antiveneno específico. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29233Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 1 (1995); 71-74 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 1 (1995); 71-74 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 1 (1995); 71-74 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29233/31090Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessRezende, Nilton Alves deDias, Mariana BorgesCampolina, DélioChavéz-Olortegui, CarlosAmaral, Carlos Faria Santos2012-07-02T01:39:41Zoai:revistas.usp.br:article/29233Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:59.071603Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus
title Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
spellingShingle Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
Rezende, Nilton Alves de
Immunodiagnosis
Scorpion envenoming
title_short Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
title_full Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
title_fullStr Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
title_full_unstemmed Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
title_sort Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus
author Rezende, Nilton Alves de
author_facet Rezende, Nilton Alves de
Dias, Mariana Borges
Campolina, Délio
Chavéz-Olortegui, Carlos
Amaral, Carlos Faria Santos
author_role author
author2 Dias, Mariana Borges
Campolina, Délio
Chavéz-Olortegui, Carlos
Amaral, Carlos Faria Santos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rezende, Nilton Alves de
Dias, Mariana Borges
Campolina, Délio
Chavéz-Olortegui, Carlos
Amaral, Carlos Faria Santos
dc.subject.por.fl_str_mv Immunodiagnosis
Scorpion envenoming
topic Immunodiagnosis
Scorpion envenoming
description The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified as mild cases and 19 as moderate or severe cases. The control absorbance in the venom assay was provided by serum samples from 100 individuals of same socioeconomic group and geographical area who had never been stung by scorpions or treated with horse antisera. The negative cutoff value (mean + 2 SD) corresponded to a venom concentration of 4.8 ng/ml. Three out of the 100 normal sera were positive, resulting in a specificity of 97%. The sensitivity of the ELISA when all cases of scorpion sting were included was 39.3%. When mild cases were excluded, the sensitivity increased to 94.7%. This study showed that this ELISA can be used for the detection of circulating venom toxic antigens in patients with systemic manifestations following. T. serrulatus sting but cannot be used for clinical studies in mild cases of envenoming since the test does not discriminate mild cases from control patients.
publishDate 1995
dc.date.none.fl_str_mv 1995-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29233
url https://www.revistas.usp.br/rimtsp/article/view/29233
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29233/31090
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 1 (1995); 71-74
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 1 (1995); 71-74
Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 1 (1995); 71-74
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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