Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/151663 |
Resumo: | Culicidae colonization in laboratory is paramount to conduct studies aiming at a better understanding of mosquitoes’ capacity to transmit pathogens that cause deadly diseases. Colonization requires female blood feeding, a necessary step for maturation of female’s oocytes. Direct blood feeding on anesthetized mammals implies in a number of disadvantages when compared to artificial blood feeding. Consequently, laboratories worldwide have been trying to -feed female mosquitoes artificially in order to replace direct feeding. In this study, we compared the effects of direct blood feeding and artificial blood feeding on important life traits of three Culicidae species. Artificial feeding was performed using citrated or defibrinated sheep blood and citrated or defibrinated rabbit blood. Direct feeding was performed using anesthetized guinea pigs as the blood source and the experiment control. Results indicated that artificial feeding using sheep blood was not good enough to justify its use in the maintenance of laboratory colonies of Culicidae. However, artificial feeding using rabbit blood maintained a recovery rate always very close to the control, especially when blood was citrated. We concluded that artificial feeding using citrated rabbit blood can substitute direct feeding on mammals reducing the use of animals, eliminating the need to maintain a bioterium in the laboratory and reducing costs in scientific researches involving Culicidae vectors. |
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Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter?CulicidaeColonizationArtificial blood feedingAnticoagulantAnimal welfareCulicidae colonization in laboratory is paramount to conduct studies aiming at a better understanding of mosquitoes’ capacity to transmit pathogens that cause deadly diseases. Colonization requires female blood feeding, a necessary step for maturation of female’s oocytes. Direct blood feeding on anesthetized mammals implies in a number of disadvantages when compared to artificial blood feeding. Consequently, laboratories worldwide have been trying to -feed female mosquitoes artificially in order to replace direct feeding. In this study, we compared the effects of direct blood feeding and artificial blood feeding on important life traits of three Culicidae species. Artificial feeding was performed using citrated or defibrinated sheep blood and citrated or defibrinated rabbit blood. Direct feeding was performed using anesthetized guinea pigs as the blood source and the experiment control. Results indicated that artificial feeding using sheep blood was not good enough to justify its use in the maintenance of laboratory colonies of Culicidae. However, artificial feeding using rabbit blood maintained a recovery rate always very close to the control, especially when blood was citrated. We concluded that artificial feeding using citrated rabbit blood can substitute direct feeding on mammals reducing the use of animals, eliminating the need to maintain a bioterium in the laboratory and reducing costs in scientific researches involving Culicidae vectors.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2018-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/151663Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e45Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e45Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e451678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/151663/148604https://www.revistas.usp.br/rimtsp/article/view/151663/148605Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessDias, Luciana dos SantosBauzeruzer, Luíz Guilherme Soares da RochaLima, José Bento Pereira2018-11-09T12:23:13Zoai:revistas.usp.br:article/151663Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:46.262709Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
title |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
spellingShingle |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? Dias, Luciana dos Santos Culicidae Colonization Artificial blood feeding Anticoagulant Animal welfare |
title_short |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
title_full |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
title_fullStr |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
title_full_unstemmed |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
title_sort |
Artificial blood feeding for Culicidae colony maintenance in laboratories: does the blood source condition matter? |
author |
Dias, Luciana dos Santos |
author_facet |
Dias, Luciana dos Santos Bauzeruzer, Luíz Guilherme Soares da Rocha Lima, José Bento Pereira |
author_role |
author |
author2 |
Bauzeruzer, Luíz Guilherme Soares da Rocha Lima, José Bento Pereira |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dias, Luciana dos Santos Bauzeruzer, Luíz Guilherme Soares da Rocha Lima, José Bento Pereira |
dc.subject.por.fl_str_mv |
Culicidae Colonization Artificial blood feeding Anticoagulant Animal welfare |
topic |
Culicidae Colonization Artificial blood feeding Anticoagulant Animal welfare |
description |
Culicidae colonization in laboratory is paramount to conduct studies aiming at a better understanding of mosquitoes’ capacity to transmit pathogens that cause deadly diseases. Colonization requires female blood feeding, a necessary step for maturation of female’s oocytes. Direct blood feeding on anesthetized mammals implies in a number of disadvantages when compared to artificial blood feeding. Consequently, laboratories worldwide have been trying to -feed female mosquitoes artificially in order to replace direct feeding. In this study, we compared the effects of direct blood feeding and artificial blood feeding on important life traits of three Culicidae species. Artificial feeding was performed using citrated or defibrinated sheep blood and citrated or defibrinated rabbit blood. Direct feeding was performed using anesthetized guinea pigs as the blood source and the experiment control. Results indicated that artificial feeding using sheep blood was not good enough to justify its use in the maintenance of laboratory colonies of Culicidae. However, artificial feeding using rabbit blood maintained a recovery rate always very close to the control, especially when blood was citrated. We concluded that artificial feeding using citrated rabbit blood can substitute direct feeding on mammals reducing the use of animals, eliminating the need to maintain a bioterium in the laboratory and reducing costs in scientific researches involving Culicidae vectors. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/151663 |
url |
https://www.revistas.usp.br/rimtsp/article/view/151663 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/151663/148604 https://www.revistas.usp.br/rimtsp/article/view/151663/148605 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e45 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e45 Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e45 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951652182458368 |