Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009

Detalhes bibliográficos
Autor(a) principal: Bisordi, Ivani
Data de Publicação: 2011
Outros Autores: Rocco, Iray Maria, Suzuki, Akemi, Katz, Gizelda, Silveira, Vivian Regina, Maeda, Adriana Yurika, Souza, Renato Pereira de, Bassi, Margarida Georgina, Del Tedesco, Eloisa Fonseca, Freitas, Eithma, Bessa, Thirsa Álvares Franco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31428
Resumo: The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes.
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spelling Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 Avaliação da detecção do antígeno NS1 para diagnóstico de dengue nos Laboratórios de Saúde Pública, Estado de São Paulo, 2009 DengueDiagnosisNS1 ELISANS1 immunochromatographic The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes. Esse estudo avaliou a acurácia do diagnóstico por detecção do antígeno NS1 do vírus Dengue empregando-se ensaios em dois formatos, imunocromatográfico e ELISA, na rotina diagnóstica dos laboratórios de Saúde Pública. Compararam-se os resultados de NS1 com os resultados de isolamento viral e, em parte dos casos, foi feita a comparação com os resultados de IgM-ELISA, obtidos nas segundas amostras. Um total de 2.321 amostras de soros, obtidas no período de março a outubro de 2009, foram analisadas por uma das duas técnicas NS1. As amostras foram divididas em cinco grupos: I, II e III, que incluíram amostras analisadas por testes NS1 e por isolamento de vírus. Os grupos IV e V incluíram pacientes com a primeira amostra processada por NS1 e segunda por IgM-ELISA. Foram analisadas sensibilidade, especificidade, valor preditivo positivo e negativo, concordância e índice Kappa. Os resultados mostraram que os grupos I, II e III apresentaram alta sensibilidade (98,0%, 99,5% e 99,3%), valores preditivos e índice Kappa entre 0,9 - 1,0. Nos grupos IV e V, apenas concordância foi calculada, dado que as amostras foram analisadas quanto à presença de antígeno NS1 ou de anticorpos IgM. Comparando-se os resultados negativos de NS1 com IgM-ELISA houve 92,1% de concordância. Com base nas constatações feitas, é possível sugerir que a detecção de NS1 pode ser importante ferramenta para monitorar a introdução e disseminação dos sorotipos de Dengue. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31428Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 6 (2011); 315-320 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 6 (2011); 315-320 Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 6 (2011); 315-320 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31428/33313Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessBisordi, IvaniRocco, Iray MariaSuzuki, AkemiKatz, GizeldaSilveira, Vivian ReginaMaeda, Adriana YurikaSouza, Renato Pereira deBassi, Margarida GeorginaDel Tedesco, Eloisa FonsecaFreitas, EithmaBessa, Thirsa Álvares Franco2012-07-07T19:42:27Zoai:revistas.usp.br:article/31428Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:06.247474Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
Avaliação da detecção do antígeno NS1 para diagnóstico de dengue nos Laboratórios de Saúde Pública, Estado de São Paulo, 2009
title Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
spellingShingle Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
Bisordi, Ivani
Dengue
Diagnosis
NS1 ELISA
NS1 immunochromatographic
title_short Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
title_full Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
title_fullStr Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
title_full_unstemmed Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
title_sort Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
author Bisordi, Ivani
author_facet Bisordi, Ivani
Rocco, Iray Maria
Suzuki, Akemi
Katz, Gizelda
Silveira, Vivian Regina
Maeda, Adriana Yurika
Souza, Renato Pereira de
Bassi, Margarida Georgina
Del Tedesco, Eloisa Fonseca
Freitas, Eithma
Bessa, Thirsa Álvares Franco
author_role author
author2 Rocco, Iray Maria
Suzuki, Akemi
Katz, Gizelda
Silveira, Vivian Regina
Maeda, Adriana Yurika
Souza, Renato Pereira de
Bassi, Margarida Georgina
Del Tedesco, Eloisa Fonseca
Freitas, Eithma
Bessa, Thirsa Álvares Franco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bisordi, Ivani
Rocco, Iray Maria
Suzuki, Akemi
Katz, Gizelda
Silveira, Vivian Regina
Maeda, Adriana Yurika
Souza, Renato Pereira de
Bassi, Margarida Georgina
Del Tedesco, Eloisa Fonseca
Freitas, Eithma
Bessa, Thirsa Álvares Franco
dc.subject.por.fl_str_mv Dengue
Diagnosis
NS1 ELISA
NS1 immunochromatographic
topic Dengue
Diagnosis
NS1 ELISA
NS1 immunochromatographic
description The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31428
url https://www.revistas.usp.br/rimtsp/article/view/31428
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31428/33313
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 6 (2011); 315-320
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 6 (2011); 315-320
Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 6 (2011); 315-320
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
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instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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