Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31428 |
Resumo: | The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes. |
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Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 Avaliação da detecção do antígeno NS1 para diagnóstico de dengue nos Laboratórios de Saúde Pública, Estado de São Paulo, 2009 DengueDiagnosisNS1 ELISANS1 immunochromatographic The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes. Esse estudo avaliou a acurácia do diagnóstico por detecção do antígeno NS1 do vírus Dengue empregando-se ensaios em dois formatos, imunocromatográfico e ELISA, na rotina diagnóstica dos laboratórios de Saúde Pública. Compararam-se os resultados de NS1 com os resultados de isolamento viral e, em parte dos casos, foi feita a comparação com os resultados de IgM-ELISA, obtidos nas segundas amostras. Um total de 2.321 amostras de soros, obtidas no período de março a outubro de 2009, foram analisadas por uma das duas técnicas NS1. As amostras foram divididas em cinco grupos: I, II e III, que incluíram amostras analisadas por testes NS1 e por isolamento de vírus. Os grupos IV e V incluíram pacientes com a primeira amostra processada por NS1 e segunda por IgM-ELISA. Foram analisadas sensibilidade, especificidade, valor preditivo positivo e negativo, concordância e índice Kappa. Os resultados mostraram que os grupos I, II e III apresentaram alta sensibilidade (98,0%, 99,5% e 99,3%), valores preditivos e índice Kappa entre 0,9 - 1,0. Nos grupos IV e V, apenas concordância foi calculada, dado que as amostras foram analisadas quanto à presença de antígeno NS1 ou de anticorpos IgM. Comparando-se os resultados negativos de NS1 com IgM-ELISA houve 92,1% de concordância. Com base nas constatações feitas, é possível sugerir que a detecção de NS1 pode ser importante ferramenta para monitorar a introdução e disseminação dos sorotipos de Dengue. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31428Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 6 (2011); 315-320 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 6 (2011); 315-320 Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 6 (2011); 315-320 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31428/33313Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessBisordi, IvaniRocco, Iray MariaSuzuki, AkemiKatz, GizeldaSilveira, Vivian ReginaMaeda, Adriana YurikaSouza, Renato Pereira deBassi, Margarida GeorginaDel Tedesco, Eloisa FonsecaFreitas, EithmaBessa, Thirsa Álvares Franco2012-07-07T19:42:27Zoai:revistas.usp.br:article/31428Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:06.247474Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 Avaliação da detecção do antígeno NS1 para diagnóstico de dengue nos Laboratórios de Saúde Pública, Estado de São Paulo, 2009 |
title |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 |
spellingShingle |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 Bisordi, Ivani Dengue Diagnosis NS1 ELISA NS1 immunochromatographic |
title_short |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 |
title_full |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 |
title_fullStr |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 |
title_full_unstemmed |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 |
title_sort |
Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009 |
author |
Bisordi, Ivani |
author_facet |
Bisordi, Ivani Rocco, Iray Maria Suzuki, Akemi Katz, Gizelda Silveira, Vivian Regina Maeda, Adriana Yurika Souza, Renato Pereira de Bassi, Margarida Georgina Del Tedesco, Eloisa Fonseca Freitas, Eithma Bessa, Thirsa Álvares Franco |
author_role |
author |
author2 |
Rocco, Iray Maria Suzuki, Akemi Katz, Gizelda Silveira, Vivian Regina Maeda, Adriana Yurika Souza, Renato Pereira de Bassi, Margarida Georgina Del Tedesco, Eloisa Fonseca Freitas, Eithma Bessa, Thirsa Álvares Franco |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bisordi, Ivani Rocco, Iray Maria Suzuki, Akemi Katz, Gizelda Silveira, Vivian Regina Maeda, Adriana Yurika Souza, Renato Pereira de Bassi, Margarida Georgina Del Tedesco, Eloisa Fonseca Freitas, Eithma Bessa, Thirsa Álvares Franco |
dc.subject.por.fl_str_mv |
Dengue Diagnosis NS1 ELISA NS1 immunochromatographic |
topic |
Dengue Diagnosis NS1 ELISA NS1 immunochromatographic |
description |
The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31428 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31428/33313 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 6 (2011); 315-320 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 6 (2011); 315-320 Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 6 (2011); 315-320 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951648034291712 |