Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1836 |
Resumo: | Capsule production is common among bacterial species, but relatively rare in eukaryotic microorganisms. Members of the fungal Cryptococcus genus are known to produce capsules, which are major determinants of virulence in the highly pathogenic species Cryptococcus neoformans and Cryptococcus gattii. Although the lack of virulence of many species of the Cryptococcus genus can be explained solely by the lack of mammalian thermotolerance, it is uncertain whether the capsules from these organisms are comparable to those of the pathogenic cryptococci. In this study, we compared the characteristic of the capsule from the non-pathogenic environmental yeast Cryptococcus liquefaciens with that of C. neoformans. Microscopic observations revealed that C. liquefaciens has a capsule visible in India ink preparations that was also efficiently labeled by three antibodies generated to specific C. neoformans capsular antigens. Capsular polysaccharides of C. liquefaciens were incorporated onto the cell surface of acapsular C. neoformans mutant cells. Polysaccharide composition determinations in combination with confocal microscopy revealed that C. liquefaciens capsule consisted of mannose, xylose, glucose, glucuronic acid, galactose and N-acetylglucosamine. Physical chemical analysis of the C. liquefaciens polysaccharides in comparison with C. neoformans samples revealed significant differences in viscosity, elastic properties and macromolecular structure parameters of polysaccharide solutions such as rigidity, effective diameter, zeta potential and molecular mass, which nevertheless appeared to be characteristics of linear polysaccharides that also comprise capsular polysaccharide of C. neoformans. The environmental yeast, however, showed enhanced susceptibility to the antimicrobial activity of the environmental phagocytes, suggesting that the C. liquefaciens capsular components are insufficient in protecting yeast cells against killing by amoeba. These results suggest that capsular structures in pathogenic Cryptococcus species and environmental species share similar features, but also manifest significant difference that could influence their potential to virulence. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCapsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells20122012-08-13T13:01:22Z2012-08-13T13:01:22ZCapsule production is common among bacterial species, but relatively rare in eukaryotic microorganisms. Members of the fungal Cryptococcus genus are known to produce capsules, which are major determinants of virulence in the highly pathogenic species Cryptococcus neoformans and Cryptococcus gattii. Although the lack of virulence of many species of the Cryptococcus genus can be explained solely by the lack of mammalian thermotolerance, it is uncertain whether the capsules from these organisms are comparable to those of the pathogenic cryptococci. In this study, we compared the characteristic of the capsule from the non-pathogenic environmental yeast Cryptococcus liquefaciens with that of C. neoformans. Microscopic observations revealed that C. liquefaciens has a capsule visible in India ink preparations that was also efficiently labeled by three antibodies generated to specific C. neoformans capsular antigens. Capsular polysaccharides of C. liquefaciens were incorporated onto the cell surface of acapsular C. neoformans mutant cells. Polysaccharide composition determinations in combination with confocal microscopy revealed that C. liquefaciens capsule consisted of mannose, xylose, glucose, glucuronic acid, galactose and N-acetylglucosamine. Physical chemical analysis of the C. liquefaciens polysaccharides in comparison with C. neoformans samples revealed significant differences in viscosity, elastic properties and macromolecular structure parameters of polysaccharide solutions such as rigidity, effective diameter, zeta potential and molecular mass, which nevertheless appeared to be characteristics of linear polysaccharides that also comprise capsular polysaccharide of C. neoformans. The environmental yeast, however, showed enhanced susceptibility to the antimicrobial activity of the environmental phagocytes, suggesting that the C. liquefaciens capsular components are insufficient in protecting yeast cells against killing by amoeba. These results suggest that capsular structures in pathogenic Cryptococcus species and environmental species share similar features, but also manifest significant difference that could influence their potential to virulence.11 p. : il.Submitted by Lívia Oliveira (livia.oliveira2@hotmail.com) on 2012-06-28T16:23:37Z No. of bitstreams: 1 Garcia_2012.pdf: 1572444 bytes, checksum: 70c7fd3535d64524869d8286f0d2f82d (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2012-06-28T16:30:08Z (GMT) No. of bitstreams: 1 Garcia_2012.pdf: 1572444 bytes, checksum: 70c7fd3535d64524869d8286f0d2f82d (MD5)Made available in DSpace on 2012-08-13T13:01:22Z (GMT). No. of bitstreams: 1 Garcia_2012.pdf: 1572444 bytes, checksum: 70c7fd3535d64524869d8286f0d2f82d (MD5) Previous issue date: 2012enghttp://hdl.handle.net/10926/1836DMD_hdl_10926/1836ARAUJO, Glauber Souza et al. Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells. Plos One, v. 7, n. 1, 2012.Araujo, Glauber de SouzaFonseca, Fernanda LopesPontes, Bruno de Almeida Carlos de CarvalhoTorres, AndreCordero, Radames J. B.Oliveira, Rosely Maria ZancopéCasadevall, ArturoViana, Nathan BessaNimrichter, LeonardoRodrigues, Marcio LourençoGarcia, Eloi de SouzaSouza, Wanderley deCarvajal, Susana Frasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROAraujo_2012.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1836/1/Araujo_2012.pdfapplication/pdf1572444http://xrepo01s.inmetro.gov.br/bitstream/10926/1836/1/Araujo_2012.pdf70c7fd3535d64524869d8286f0d2f82dMD510926_1836_1Araujo_2012.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1836/6/Araujo_2012.pdf.txttext/plain55975http://xrepo01s.inmetro.gov.br/bitstream/10926/1836/6/Araujo_2012.pdf.txt65ffa9db422d9ab778b251258b411ab1MD510926_1836_62024-06-10T15:24:53Zoai:xrepo01s.inmetro.gov.br:10926/1836Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2013-05-24T16:06:09Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
title |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
spellingShingle |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells Araujo, Glauber de Souza |
title_short |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
title_full |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
title_fullStr |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
title_full_unstemmed |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
title_sort |
Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells |
author |
Araujo, Glauber de Souza |
author_facet |
Araujo, Glauber de Souza Fonseca, Fernanda Lopes Pontes, Bruno de Almeida Carlos de Carvalho Torres, Andre Cordero, Radames J. B. Oliveira, Rosely Maria Zancopé Casadevall, Arturo Viana, Nathan Bessa Nimrichter, Leonardo Rodrigues, Marcio Lourenço Garcia, Eloi de Souza Souza, Wanderley de Carvajal, Susana Frases |
author_role |
author |
author2 |
Fonseca, Fernanda Lopes Pontes, Bruno de Almeida Carlos de Carvalho Torres, Andre Cordero, Radames J. B. Oliveira, Rosely Maria Zancopé Casadevall, Arturo Viana, Nathan Bessa Nimrichter, Leonardo Rodrigues, Marcio Lourenço Garcia, Eloi de Souza Souza, Wanderley de Carvajal, Susana Frases |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Araujo, Glauber de Souza Fonseca, Fernanda Lopes Pontes, Bruno de Almeida Carlos de Carvalho Torres, Andre Cordero, Radames J. B. Oliveira, Rosely Maria Zancopé Casadevall, Arturo Viana, Nathan Bessa Nimrichter, Leonardo Rodrigues, Marcio Lourenço Garcia, Eloi de Souza Souza, Wanderley de Carvajal, Susana Frases |
description |
Capsule production is common among bacterial species, but relatively rare in eukaryotic microorganisms. Members of the fungal Cryptococcus genus are known to produce capsules, which are major determinants of virulence in the highly pathogenic species Cryptococcus neoformans and Cryptococcus gattii. Although the lack of virulence of many species of the Cryptococcus genus can be explained solely by the lack of mammalian thermotolerance, it is uncertain whether the capsules from these organisms are comparable to those of the pathogenic cryptococci. In this study, we compared the characteristic of the capsule from the non-pathogenic environmental yeast Cryptococcus liquefaciens with that of C. neoformans. Microscopic observations revealed that C. liquefaciens has a capsule visible in India ink preparations that was also efficiently labeled by three antibodies generated to specific C. neoformans capsular antigens. Capsular polysaccharides of C. liquefaciens were incorporated onto the cell surface of acapsular C. neoformans mutant cells. Polysaccharide composition determinations in combination with confocal microscopy revealed that C. liquefaciens capsule consisted of mannose, xylose, glucose, glucuronic acid, galactose and N-acetylglucosamine. Physical chemical analysis of the C. liquefaciens polysaccharides in comparison with C. neoformans samples revealed significant differences in viscosity, elastic properties and macromolecular structure parameters of polysaccharide solutions such as rigidity, effective diameter, zeta potential and molecular mass, which nevertheless appeared to be characteristics of linear polysaccharides that also comprise capsular polysaccharide of C. neoformans. The environmental yeast, however, showed enhanced susceptibility to the antimicrobial activity of the environmental phagocytes, suggesting that the C. liquefaciens capsular components are insufficient in protecting yeast cells against killing by amoeba. These results suggest that capsular structures in pathogenic Cryptococcus species and environmental species share similar features, but also manifest significant difference that could influence their potential to virulence. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.available.fl_str_mv |
2012-08-13T13:01:22Z |
dc.date.accessioned.fl_str_mv |
2012-08-13T13:01:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1836 DMD_hdl_10926/1836 |
dc.identifier.citation.fl_str_mv |
ARAUJO, Glauber Souza et al. Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells. Plos One, v. 7, n. 1, 2012. |
url |
http://hdl.handle.net/10926/1836 |
identifier_str_mv |
DMD_hdl_10926/1836 ARAUJO, Glauber Souza et al. Capsules from pathogenic and non-pathogenic cryptococcus spp. manifest significant differences in structure and ability to protect against phagocytic cells. Plos One, v. 7, n. 1, 2012. |
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eng |
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