Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats

• Autor(a) principal: Benatti, B. B.
• Data de Publicação: 2012
• Outros Autores: Campos-Júnior, Jozafá C., Silva-Filho, Vilmar J., Alves, Polyanna Miranda, Rodrigues, Isabela R., Uber-Bucek, Elizabeth, Manfredo Vieira, Silvio, Napimoga, Marcelo Henrique
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/16153

Objectives: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. Material and Methods: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. Results: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. Conclusions: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.