Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/46241 |
Resumo: | OBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats. |
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Journal of applied oral science (Online) |
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Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in ratsGuacoPeriodontitisRatsOBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.Universidade de São Paulo. Faculdade de Odontologia de Bauru2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/4624110.1590/S1678-77572012000300008Journal of Applied Oral Science; Vol. 20 No. 3 (2012); 340-346Journal of Applied Oral Science; Vol. 20 Núm. 3 (2012); 340-346Journal of Applied Oral Science; v. 20 n. 3 (2012); 340-3461678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/46241/49863Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessBenatti, Bruno B.Campos-Júnior, Jozafá C.Silva-Filho, Vilmar J.Alves, Polyanna M.Rodrigues, Isabela R.Uber-Bucek, ElizabethVieira, Silvio M.Napimoga, Marcelo H.2012-10-18T19:37:40Zoai:revistas.usp.br:article/46241Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-10-18T19:37:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
spellingShingle |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats Benatti, Bruno B. Guaco Periodontitis Rats |
title_short |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_full |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_fullStr |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_full_unstemmed |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_sort |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
author |
Benatti, Bruno B. |
author_facet |
Benatti, Bruno B. Campos-Júnior, Jozafá C. Silva-Filho, Vilmar J. Alves, Polyanna M. Rodrigues, Isabela R. Uber-Bucek, Elizabeth Vieira, Silvio M. Napimoga, Marcelo H. |
author_role |
author |
author2 |
Campos-Júnior, Jozafá C. Silva-Filho, Vilmar J. Alves, Polyanna M. Rodrigues, Isabela R. Uber-Bucek, Elizabeth Vieira, Silvio M. Napimoga, Marcelo H. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Benatti, Bruno B. Campos-Júnior, Jozafá C. Silva-Filho, Vilmar J. Alves, Polyanna M. Rodrigues, Isabela R. Uber-Bucek, Elizabeth Vieira, Silvio M. Napimoga, Marcelo H. |
dc.subject.por.fl_str_mv |
Guaco Periodontitis Rats |
topic |
Guaco Periodontitis Rats |
description |
OBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/46241 10.1590/S1678-77572012000300008 |
url |
https://www.revistas.usp.br/jaos/article/view/46241 |
identifier_str_mv |
10.1590/S1678-77572012000300008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/46241/49863 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 20 No. 3 (2012); 340-346 Journal of Applied Oral Science; Vol. 20 Núm. 3 (2012); 340-346 Journal of Applied Oral Science; v. 20 n. 3 (2012); 340-346 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221677431291904 |