Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats

Detalhes bibliográficos
Autor(a) principal: Benatti, Bruno B.
Data de Publicação: 2012
Outros Autores: Campos-Júnior, Jozafá C., Silva-Filho, Vilmar J., Alves, Polyanna M., Rodrigues, Isabela R., Uber-Bucek, Elizabeth, Vieira, Silvio M., Napimoga, Marcelo H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/46241
Resumo: OBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.
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spelling Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in ratsGuacoPeriodontitisRatsOBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.Universidade de São Paulo. Faculdade de Odontologia de Bauru2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/4624110.1590/S1678-77572012000300008Journal of Applied Oral Science; Vol. 20 No. 3 (2012); 340-346Journal of Applied Oral Science; Vol. 20 Núm. 3 (2012); 340-346Journal of Applied Oral Science; v. 20 n. 3 (2012); 340-3461678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/46241/49863Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessBenatti, Bruno B.Campos-Júnior, Jozafá C.Silva-Filho, Vilmar J.Alves, Polyanna M.Rodrigues, Isabela R.Uber-Bucek, ElizabethVieira, Silvio M.Napimoga, Marcelo H.2012-10-18T19:37:40Zoai:revistas.usp.br:article/46241Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-10-18T19:37:40Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
title Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
spellingShingle Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
Benatti, Bruno B.
Guaco
Periodontitis
Rats
title_short Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
title_full Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
title_fullStr Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
title_full_unstemmed Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
title_sort Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
author Benatti, Bruno B.
author_facet Benatti, Bruno B.
Campos-Júnior, Jozafá C.
Silva-Filho, Vilmar J.
Alves, Polyanna M.
Rodrigues, Isabela R.
Uber-Bucek, Elizabeth
Vieira, Silvio M.
Napimoga, Marcelo H.
author_role author
author2 Campos-Júnior, Jozafá C.
Silva-Filho, Vilmar J.
Alves, Polyanna M.
Rodrigues, Isabela R.
Uber-Bucek, Elizabeth
Vieira, Silvio M.
Napimoga, Marcelo H.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Benatti, Bruno B.
Campos-Júnior, Jozafá C.
Silva-Filho, Vilmar J.
Alves, Polyanna M.
Rodrigues, Isabela R.
Uber-Bucek, Elizabeth
Vieira, Silvio M.
Napimoga, Marcelo H.
dc.subject.por.fl_str_mv Guaco
Periodontitis
Rats
topic Guaco
Periodontitis
Rats
description OBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/46241
10.1590/S1678-77572012000300008
url https://www.revistas.usp.br/jaos/article/view/46241
identifier_str_mv 10.1590/S1678-77572012000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/46241/49863
dc.rights.driver.fl_str_mv Copyright (c) 2012 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2012 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 20 No. 3 (2012); 340-346
Journal of Applied Oral Science; Vol. 20 Núm. 3 (2012); 340-346
Journal of Applied Oral Science; v. 20 n. 3 (2012); 340-346
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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