Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16153 |
Resumo: | Objectives: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. Material and Methods: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. Results: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. Conclusions: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats. |
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Benatti, B. B.Campos-Júnior, Jozafá C.Silva-Filho, Vilmar J.Alves, Polyanna MirandaRodrigues, Isabela R.Uber-Bucek, ElizabethManfredo Vieira, SilvioNapimoga, Marcelo Henrique2020-05-25T19:10:48Z2020-05-25T19:10:48Z2012https://repositorio.inpa.gov.br/handle/1/1615310.1590/S1678-77572012000300008Objectives: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. Material and Methods: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. Results: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. Conclusions: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.Volume 20, Número 3, Pags. 340-346Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessEffects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Applied Oral Scienceengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf774331https://repositorio.inpa.gov.br/bitstream/1/16153/1/artigo-inpa.pdf3a5fff7e76fd206f793cb36967c31753MD511/161532020-05-25 16:34:32.005oai:repositorio:1/16153Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-25T20:34:32Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
spellingShingle |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats Benatti, B. B. |
title_short |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_full |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_fullStr |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_full_unstemmed |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
title_sort |
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats |
author |
Benatti, B. B. |
author_facet |
Benatti, B. B. Campos-Júnior, Jozafá C. Silva-Filho, Vilmar J. Alves, Polyanna Miranda Rodrigues, Isabela R. Uber-Bucek, Elizabeth Manfredo Vieira, Silvio Napimoga, Marcelo Henrique |
author_role |
author |
author2 |
Campos-Júnior, Jozafá C. Silva-Filho, Vilmar J. Alves, Polyanna Miranda Rodrigues, Isabela R. Uber-Bucek, Elizabeth Manfredo Vieira, Silvio Napimoga, Marcelo Henrique |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Benatti, B. B. Campos-Júnior, Jozafá C. Silva-Filho, Vilmar J. Alves, Polyanna Miranda Rodrigues, Isabela R. Uber-Bucek, Elizabeth Manfredo Vieira, Silvio Napimoga, Marcelo Henrique |
description |
Objectives: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. Material and Methods: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. Results: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. Conclusions: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2020-05-25T19:10:48Z |
dc.date.available.fl_str_mv |
2020-05-25T19:10:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16153 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1678-77572012000300008 |
url |
https://repositorio.inpa.gov.br/handle/1/16153 |
identifier_str_mv |
10.1590/S1678-77572012000300008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 20, Número 3, Pags. 340-346 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Journal of Applied Oral Science |
publisher.none.fl_str_mv |
Journal of Applied Oral Science |
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reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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