Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

Detalhes bibliográficos
Autor(a) principal: Marinheiro, Juliana Cristina
Data de Publicação: 2009
Outros Autores: Sanalios, Roberta Braga, dos Santos, Daniela Carvalho, Costa, Cristóvão Alves da, Hársi, Charlotte Marianna
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14517
Resumo: Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
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spelling Marinheiro, Juliana CristinaSanalios, Roberta Bragados Santos, Daniela CarvalhoCosta, Cristóvão Alves daHársi, Charlotte Marianna2020-04-24T16:49:25Z2020-04-24T16:49:25Z2009https://repositorio.inpa.gov.br/handle/1/1451710.1590/S0074-02762009000100018Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.Volume 104, Número 1, Pags. 118-120Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAdenoviridaeHuman AdenovirusHuman Respiratory Syncytial VirusHydrangea Ringspot VirusRespiratory Syncytial VirusAdenoviridaeHuman AdenovirusHuman Respiratory Syncytial VirusHydrangea Ringspot VirusRespiratory Syncytial VirusAcute DiseaseAdenovirusAdenovirus InfectionCase Control StudyChildGeneticsHumanIsolation And PurificationMethodologyNasopharynxPolymerase Chain ReactionRespiratory Syncytial PneumovirusRespiratory Tract InfectionSensitivity And SpecificityVirologyVirus InfectionAcute DiseaseAdenoviridaeAdenoviridae InfectionsCase-control StudiesChildHumansNasopharynxPolymerase Chain ReactionRespiratory Syncytial Virus InfectionsHuman Respiratory Syncytial VirusRespiratory Tract InfectionsSensitivity And SpecificityDuplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMemórias do Instituto Oswaldo Cruzengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf323333https://repositorio.inpa.gov.br/bitstream/1/14517/1/artigo-inpa.pdf979dafefd92f4f74299494e68376ee1eMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14517/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/145172020-07-14 09:04:46.052oai:repositorio:1/14517Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T13:04:46Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
spellingShingle Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Marinheiro, Juliana Cristina
Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Acute Disease
Adenovirus
Adenovirus Infection
Case Control Study
Child
Genetics
Human
Isolation And Purification
Methodology
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Pneumovirus
Respiratory Tract Infection
Sensitivity And Specificity
Virology
Virus Infection
Acute Disease
Adenoviridae
Adenoviridae Infections
Case-control Studies
Child
Humans
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Human Respiratory Syncytial Virus
Respiratory Tract Infections
Sensitivity And Specificity
title_short Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_full Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_fullStr Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_full_unstemmed Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_sort Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
author Marinheiro, Juliana Cristina
author_facet Marinheiro, Juliana Cristina
Sanalios, Roberta Braga
dos Santos, Daniela Carvalho
Costa, Cristóvão Alves da
Hársi, Charlotte Marianna
author_role author
author2 Sanalios, Roberta Braga
dos Santos, Daniela Carvalho
Costa, Cristóvão Alves da
Hársi, Charlotte Marianna
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Marinheiro, Juliana Cristina
Sanalios, Roberta Braga
dos Santos, Daniela Carvalho
Costa, Cristóvão Alves da
Hársi, Charlotte Marianna
dc.subject.eng.fl_str_mv Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Acute Disease
Adenovirus
Adenovirus Infection
Case Control Study
Child
Genetics
Human
Isolation And Purification
Methodology
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Pneumovirus
Respiratory Tract Infection
Sensitivity And Specificity
Virology
Virus Infection
Acute Disease
Adenoviridae
Adenoviridae Infections
Case-control Studies
Child
Humans
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Human Respiratory Syncytial Virus
Respiratory Tract Infections
Sensitivity And Specificity
topic Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Acute Disease
Adenovirus
Adenovirus Infection
Case Control Study
Child
Genetics
Human
Isolation And Purification
Methodology
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Pneumovirus
Respiratory Tract Infection
Sensitivity And Specificity
Virology
Virus Infection
Acute Disease
Adenoviridae
Adenoviridae Infections
Case-control Studies
Child
Humans
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Human Respiratory Syncytial Virus
Respiratory Tract Infections
Sensitivity And Specificity
description Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2020-04-24T16:49:25Z
dc.date.available.fl_str_mv 2020-04-24T16:49:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14517
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762009000100018
url https://repositorio.inpa.gov.br/handle/1/14517
identifier_str_mv 10.1590/S0074-02762009000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 104, Número 1, Pags. 118-120
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
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