Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1819 |
Resumo: | Interactions between the muscle and macrophages which invade it after an injury occurs are crucial to the evolution of repair in this tissue. On the other hand, low intensity laser (LLLT) has long been used in the treatment of such lesions, both in clinical and experimental settings; however, little is known about its effects on macrophages. The objective of this project was to evaluate the effect of irradiation with red and infrared laser on the activation state of M1 and M2a phenotypes of macrophages. For this purpose, J774 macrophage cultures were treated with polarizing agents (LPS and IFN-y or IL-4) for 24 hours and were then irradiated with 2 wavelengths of LLLT ??? 780 nm and 660 nm ??? in the same dosimetric parameters (70mW; 17.5J/cm2; 0.8J). After 24 hours of incubation, the activation state was checked by MTT technique. In each experimental situation, non-irradiated cells served as controls, and three independent experiments were performed. Laser irradiation with 780 nm proved able to decrease the activation of macrophages polarized for M1 phenotype and increase activation of M2a profile macrophages. Laser irradiation with 660 nm slightly intensified the state and activation of M1 macrophages, while significantly increasing the activation state of M2a profile macrophages. Although it is not possible to establish a relationship between the results of in vitro studies and future clinical outcomes, and the effects of laser irradiation on other macrophage phenotypes still need to be assessed, data from this study suggest that red and infrared laser could have different results when used in the different stages of muscle repair. |
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Fernandes, Kristianne Porta SantosFernandes, Kristianne Porta SantosFerrari, Raquel Agnelli MesquitaIemma, Monica Rosas da Costahttp://lattes.cnpq.br/1034065650890955Almeida, Terezinha Aparecida de2018-06-19T18:27:35Z2015-12-16Almeida, Terezinha Aparecida de. Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular. 2015. 42 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1819Interactions between the muscle and macrophages which invade it after an injury occurs are crucial to the evolution of repair in this tissue. On the other hand, low intensity laser (LLLT) has long been used in the treatment of such lesions, both in clinical and experimental settings; however, little is known about its effects on macrophages. The objective of this project was to evaluate the effect of irradiation with red and infrared laser on the activation state of M1 and M2a phenotypes of macrophages. For this purpose, J774 macrophage cultures were treated with polarizing agents (LPS and IFN-y or IL-4) for 24 hours and were then irradiated with 2 wavelengths of LLLT ??? 780 nm and 660 nm ??? in the same dosimetric parameters (70mW; 17.5J/cm2; 0.8J). After 24 hours of incubation, the activation state was checked by MTT technique. In each experimental situation, non-irradiated cells served as controls, and three independent experiments were performed. Laser irradiation with 780 nm proved able to decrease the activation of macrophages polarized for M1 phenotype and increase activation of M2a profile macrophages. Laser irradiation with 660 nm slightly intensified the state and activation of M1 macrophages, while significantly increasing the activation state of M2a profile macrophages. Although it is not possible to establish a relationship between the results of in vitro studies and future clinical outcomes, and the effects of laser irradiation on other macrophage phenotypes still need to be assessed, data from this study suggest that red and infrared laser could have different results when used in the different stages of muscle repair.As intera????es entre o m??sculo e os macr??fagos que o invadem ap??s a ocorr??ncia da les??o s??o determinantes para a evolu????o do reparo deste tecido. Por outro lado, o laser em baixa intensidade (LBI) tem sido muito utilizado no tratamento destas les??es no ??mbito cl??nico e no experimental, mas pouco se conhece a respeito dos seus efeitos sobre os macr??fagos. O objetivo deste projeto foi avaliar o efeito da irradia????o com laser vermelho e infravermelho sobre o estado de ativa????o de macr??fagos de fen??tipo M1 e M2a. Para tanto, culturas de macr??fagos J774 foram tratadas com agentes polarizadores (LPS e IFN-y ou IL-4) por 24h e ent??o irradiadas com LBI em 2 comprimentos de onda 780nm e 660nm nos mesmos par??metros dosim??tricos (70mW; 17,5J/cm2; 0,8J). Ap??s 24h de incuba????o, o estado de ativa????o foi verificado por meio da t??cnica MTT. Em cada situa????o experimental, c??lulas n??o irradiadas serviram como controle, sendo realizados tr??s experimentos independentes. A irradia????o com laser de 780 nm mostrou-se capaz de diminuir a ativa????o de macr??fagos polarizados para fen??tipo M1 e aumentar a ativa????o de macr??fagos de perfil M2a. J?? a irradia????o com laser de 660 nm intensificou ligeiramente o estado e ativa????o dos macr??fagos M1 e aumentou de maneira significante o estado de ativa????o de macr??fagos de perfil M2a. Embora n??o se possa estabelecer uma rela????o entre resultados de estudos in vitro e desfechos cl??nicos futuros e ainda exista necessidade de avaliar os efeitos da irradia????o laser nos outros fen??tipos de macr??fagos, os dados do presente estudo sugerem que os lasers vermelho e infravermelho pode trazer resultados diferentes quando utilizados nas diferentes fases do reparo muscular.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-19T18:27:35Z No. of bitstreams: 1 Terezinha Aparecida de Almeida.pdf: 739598 bytes, checksum: 0b0c7aabccbc5f5868d542f6e63d53f0 (MD5)Made available in DSpace on 2018-06-19T18:27:35Z (GMT). No. of bitstreams: 1 Terezinha Aparecida de Almeida.pdf: 739598 bytes, checksum: 0b0c7aabccbc5f5868d542f6e63d53f0 (MD5) Previous issue date: 2015-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??demacr??fagoslaser de baixa pot??nciales??o muscularmacrophagelow level lasermuscle injuryCIENCIAS DA SAUDEEfeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscularEffect of red and infrared lasers on the activation of the cells responsible for muscle repairinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALTerezinha Aparecida de Almeida.pdfTerezinha Aparecida de Almeida.pdfapplication/pdf739598http://localhost:8080/tede/bitstream/tede/1819/2/Terezinha+Aparecida+de+Almeida.pdf0b0c7aabccbc5f5868d542f6e63d53f0MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1819/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18192018-06-19 15:27:35.535oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-19T18:27:35Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
dc.title.alternative.eng.fl_str_mv |
Effect of red and infrared lasers on the activation of the cells responsible for muscle repair |
title |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
spellingShingle |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular Almeida, Terezinha Aparecida de macr??fagos laser de baixa pot??ncia les??o muscular macrophage low level laser muscle injury CIENCIAS DA SAUDE |
title_short |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
title_full |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
title_fullStr |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
title_full_unstemmed |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
title_sort |
Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular |
author |
Almeida, Terezinha Aparecida de |
author_facet |
Almeida, Terezinha Aparecida de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.referee1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.referee2.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee3.fl_str_mv |
Iemma, Monica Rosas da Costa |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1034065650890955 |
dc.contributor.author.fl_str_mv |
Almeida, Terezinha Aparecida de |
contributor_str_mv |
Fernandes, Kristianne Porta Santos Fernandes, Kristianne Porta Santos Ferrari, Raquel Agnelli Mesquita Iemma, Monica Rosas da Costa |
dc.subject.por.fl_str_mv |
macr??fagos laser de baixa pot??ncia les??o muscular |
topic |
macr??fagos laser de baixa pot??ncia les??o muscular macrophage low level laser muscle injury CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
macrophage low level laser muscle injury |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Interactions between the muscle and macrophages which invade it after an injury occurs are crucial to the evolution of repair in this tissue. On the other hand, low intensity laser (LLLT) has long been used in the treatment of such lesions, both in clinical and experimental settings; however, little is known about its effects on macrophages. The objective of this project was to evaluate the effect of irradiation with red and infrared laser on the activation state of M1 and M2a phenotypes of macrophages. For this purpose, J774 macrophage cultures were treated with polarizing agents (LPS and IFN-y or IL-4) for 24 hours and were then irradiated with 2 wavelengths of LLLT ??? 780 nm and 660 nm ??? in the same dosimetric parameters (70mW; 17.5J/cm2; 0.8J). After 24 hours of incubation, the activation state was checked by MTT technique. In each experimental situation, non-irradiated cells served as controls, and three independent experiments were performed. Laser irradiation with 780 nm proved able to decrease the activation of macrophages polarized for M1 phenotype and increase activation of M2a profile macrophages. Laser irradiation with 660 nm slightly intensified the state and activation of M1 macrophages, while significantly increasing the activation state of M2a profile macrophages. Although it is not possible to establish a relationship between the results of in vitro studies and future clinical outcomes, and the effects of laser irradiation on other macrophage phenotypes still need to be assessed, data from this study suggest that red and infrared laser could have different results when used in the different stages of muscle repair. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-12-16 |
dc.date.accessioned.fl_str_mv |
2018-06-19T18:27:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Almeida, Terezinha Aparecida de. Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular. 2015. 42 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1819 |
identifier_str_mv |
Almeida, Terezinha Aparecida de. Efeito dos lasers vermelho e infravermelho sobre a ativa????o das c??lulas respons??veis pelo direcionamento do reparo muscular. 2015. 42 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1819 |
dc.language.iso.fl_str_mv |
por |
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por |
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8765449414823306929 |
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600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
dc.publisher.initials.fl_str_mv |
UNINOVE |
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Brasil |
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Sa??de |
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Universidade Nove de Julho |
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