A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1302 |
Resumo: | Myocardial infarction (MI) in rats promoted by occlusion of the anterior descending coronary artery is the most widely used experimental model in research that seeks to elucidate the functional, structural and molecular changes associated with ischemic heart disease. Recently, low-level laser therapy (LLLT) has become an alternative therapy to modulate various biological processes and depending on the wavelength, dosage and condition of the irradiated tissue, has anti-inflammatory effect, reduces pain and accelerate cell proliferation. Thus, we evaluated in experimental rats that underwent coronary artery occlusion after irradiation with therapeutic laser myocardial function by echocardiography. Also an analysis was carried out "in-silico", using bioinformatics tools to verify genes involved in metabolic pathways related to MI in rats. After searching the databases, 47 genes of 9 metabolic pathways (Angiogenesis and cell survival, hypertrophy, oxidative stress, apoptosis, extracellular matrix, the C??lcio kinetics, cell metabolism and inflammation) were associated with MI. The changes in gene expression that occur in the myocardium were analyzed by PCR array Real Time. The Group Myocardial Infarction (MI), and the Group Infarction + Laser (MI + Laser) when compared to the control group (Sham), showed 22 (44.60%) and 12 (25.53%) of differentially expressed genes respectively. The results of this study suggest that laser therapy was able to modulate various post-infarction processes, including apoptosis, inflammation, cardiac hypertrophy, cell metabolism and especially the ECM composition in cardiac remodeling. The data obtained here, although complex, indicate genes of interest and certainly allow further analysis of potential key genes in cellular response. Thus, this screening of the initial expression profiles ensures more in-depth studies to confirm and explain the role of each gene or group of genes involved in acute myocardial infarction. |
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Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161Mori, Marcelo Alves da Silvahttp://lattes.cnpq.br/3425809117024031Giannella Neto, Danielhttp://lattes.cnpq.br/6028188157816694http://lattes.cnpq.br/9239261313445770Feliciano, Regiane dos Santos2016-05-18T14:38:01Z2015-02-19Feliciano, Regiane dos Santos. A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica. 2015. 105 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1302Myocardial infarction (MI) in rats promoted by occlusion of the anterior descending coronary artery is the most widely used experimental model in research that seeks to elucidate the functional, structural and molecular changes associated with ischemic heart disease. Recently, low-level laser therapy (LLLT) has become an alternative therapy to modulate various biological processes and depending on the wavelength, dosage and condition of the irradiated tissue, has anti-inflammatory effect, reduces pain and accelerate cell proliferation. Thus, we evaluated in experimental rats that underwent coronary artery occlusion after irradiation with therapeutic laser myocardial function by echocardiography. Also an analysis was carried out "in-silico", using bioinformatics tools to verify genes involved in metabolic pathways related to MI in rats. After searching the databases, 47 genes of 9 metabolic pathways (Angiogenesis and cell survival, hypertrophy, oxidative stress, apoptosis, extracellular matrix, the C??lcio kinetics, cell metabolism and inflammation) were associated with MI. The changes in gene expression that occur in the myocardium were analyzed by PCR array Real Time. The Group Myocardial Infarction (MI), and the Group Infarction + Laser (MI + Laser) when compared to the control group (Sham), showed 22 (44.60%) and 12 (25.53%) of differentially expressed genes respectively. The results of this study suggest that laser therapy was able to modulate various post-infarction processes, including apoptosis, inflammation, cardiac hypertrophy, cell metabolism and especially the ECM composition in cardiac remodeling. The data obtained here, although complex, indicate genes of interest and certainly allow further analysis of potential key genes in cellular response. Thus, this screening of the initial expression profiles ensures more in-depth studies to confirm and explain the role of each gene or group of genes involved in acute myocardial infarction.O infarto do mioc??rdio (IM) promovido no rato por oclus??o da art??ria coron??ria descendente anterior esquerda ?? o modelo experimental mais empregado em pesquisas que visa elucidar as modifica????es funcionais, estruturais e moleculares associadas ?? doen??a card??aca isqu??mica. Recentemente, o laser de baixa intensidade (LBI) tem se tornado uma alternativa terap??utica por modular v??rios processos biol??gicos e, dependendo do comprimento de onda, dose e condi????o do tecido irradiado, possui efeito anti-inflamat??rio, reduz a dor e acelera a prolifera????o celular. Assim, avaliamos em modelo experimental em ratas, que foram submetidos ?? oclus??o da art??ria coron??ria ap??s irradia????o com laser terap??utico a fun????o mioc??rdica por ecocardiograma. Al??m disso, realizamos uma an??lise ???in silico???, utilizando ferramentas de Bioinform??tica para verificar genes envolvidos em vias metab??licas relacionadas ao IM em ratos. Ap??s busca nos bancos de dados, 47 genes de 9 vias metab??licas (Angiog??nese e sobrevida celular, Hipertrofia, Estresse oxidativo, Apoptose, Matriz Extracelular, Cin??tica do C??lcio, Metabolismo celular e Inflama????o) foram associados ao IM. As modifica????es da express??o g??nica que possam ocorrer no mioc??rdio foram analisadas por PCR ???ARRAY em Tempo Real???. O Grupo Infarto do Mioc??rdio (IM), e o Grupo Infarto + Laser (IM+Laser), quando comparados ao grupo controle (Sham), apresentaram 22 (44,60%) e 12 (25,53%) de genes diferencialmente expressos respectivamente. Os resultados encontrados neste trabalho sugerem que a laserterapia foi capaz de modular v??rios processos p??s-infarto, entre eles a apoptose, processos inflamat??rios, a hipertrofia card??aca, o metabolismo celular e especialmente a composi????o da MEC no remodelamento card??aco. Os dados aqui obtidos, embora complexos, sinalizam genes de interesse e certamente permitem an??lises mais aprofundadas de potenciais genes chaves na resposta celular. Assim, esse ???screening??? inicial dos perfis de express??o garante outros estudos mais aprofundados para confirmar e explicar o papel de cada gene ou grupo de genes envolvidos no Infarto agudo do Mioc??rdio.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2016-05-18T14:38:01Z No. of bitstreams: 1 Regiane dos Santos Feliciano.pdf: 3100459 bytes, checksum: d08af42a7ecbfdd398b61020916eebd6 (MD5)Made available in DSpace on 2016-05-18T14:38:01Z (GMT). No. of bitstreams: 1 Regiane dos Santos Feliciano.pdf: 3100459 bytes, checksum: d08af42a7ecbfdd398b61020916eebd6 (MD5) Previous issue date: 2015-02-19application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSa??deinfarto do mioc??rdiolaserterapialaser de baixa pot??nciaexpress??o g??nicamyocardial infarctionlaser therapylow level laser therapygene expressionCIENCIAS DA SAUDEA????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nicaLaser therapy of action in myocardial infarction induced coronary artery occlusion in rats: analysis of gene expression profileinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALRegiane dos Santos Feliciano.pdfRegiane dos Santos Feliciano.pdfapplication/pdf3100459http://localhost:8080/tede/bitstream/tede/1302/2/Regiane+dos+Santos+Feliciano.pdfd08af42a7ecbfdd398b61020916eebd6MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1302/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/13022017-05-26 17:51:46.336oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2017-05-26T20:51:46Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
dc.title.alternative.eng.fl_str_mv |
Laser therapy of action in myocardial infarction induced coronary artery occlusion in rats: analysis of gene expression profile |
title |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
spellingShingle |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica Feliciano, Regiane dos Santos infarto do mioc??rdio laserterapia laser de baixa pot??ncia express??o g??nica myocardial infarction laser therapy low level laser therapy gene expression CIENCIAS DA SAUDE |
title_short |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
title_full |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
title_fullStr |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
title_full_unstemmed |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
title_sort |
A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica |
author |
Feliciano, Regiane dos Santos |
author_facet |
Feliciano, Regiane dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva Junior, Jose Antonio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1990427833073161 |
dc.contributor.referee1.fl_str_mv |
Silva Junior, Jose Antonio |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1990427833073161 |
dc.contributor.referee2.fl_str_mv |
Mori, Marcelo Alves da Silva |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3425809117024031 |
dc.contributor.referee3.fl_str_mv |
Giannella Neto, Daniel |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/6028188157816694 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9239261313445770 |
dc.contributor.author.fl_str_mv |
Feliciano, Regiane dos Santos |
contributor_str_mv |
Silva Junior, Jose Antonio Silva Junior, Jose Antonio Mori, Marcelo Alves da Silva Giannella Neto, Daniel |
dc.subject.por.fl_str_mv |
infarto do mioc??rdio laserterapia laser de baixa pot??ncia express??o g??nica |
topic |
infarto do mioc??rdio laserterapia laser de baixa pot??ncia express??o g??nica myocardial infarction laser therapy low level laser therapy gene expression CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
myocardial infarction laser therapy low level laser therapy gene expression |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Myocardial infarction (MI) in rats promoted by occlusion of the anterior descending coronary artery is the most widely used experimental model in research that seeks to elucidate the functional, structural and molecular changes associated with ischemic heart disease. Recently, low-level laser therapy (LLLT) has become an alternative therapy to modulate various biological processes and depending on the wavelength, dosage and condition of the irradiated tissue, has anti-inflammatory effect, reduces pain and accelerate cell proliferation. Thus, we evaluated in experimental rats that underwent coronary artery occlusion after irradiation with therapeutic laser myocardial function by echocardiography. Also an analysis was carried out "in-silico", using bioinformatics tools to verify genes involved in metabolic pathways related to MI in rats. After searching the databases, 47 genes of 9 metabolic pathways (Angiogenesis and cell survival, hypertrophy, oxidative stress, apoptosis, extracellular matrix, the C??lcio kinetics, cell metabolism and inflammation) were associated with MI. The changes in gene expression that occur in the myocardium were analyzed by PCR array Real Time. The Group Myocardial Infarction (MI), and the Group Infarction + Laser (MI + Laser) when compared to the control group (Sham), showed 22 (44.60%) and 12 (25.53%) of differentially expressed genes respectively. The results of this study suggest that laser therapy was able to modulate various post-infarction processes, including apoptosis, inflammation, cardiac hypertrophy, cell metabolism and especially the ECM composition in cardiac remodeling. The data obtained here, although complex, indicate genes of interest and certainly allow further analysis of potential key genes in cellular response. Thus, this screening of the initial expression profiles ensures more in-depth studies to confirm and explain the role of each gene or group of genes involved in acute myocardial infarction. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-19 |
dc.date.accessioned.fl_str_mv |
2016-05-18T14:38:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Feliciano, Regiane dos Santos. A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica. 2015. 105 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1302 |
identifier_str_mv |
Feliciano, Regiane dos Santos. A????o da laserterapia no infarto do mioc??rdio induzido por oclus??o da art??ria coron??ria em ratas: an??lise do perfil de express??o g??nica. 2015. 105 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/1302 |
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