Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)

Detalhes bibliográficos
Autor(a) principal: Basso, Juliana Gerunda
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/tede/handle/tede/811
Resumo: The present study was conducted to evaluate the activity of enzymatic components of saliva and possible correlations with clinical parameters in patients with COPD. The sample consisted of 30 individuals, 20 individuals with COPD and 10 controls. The clinical parameters of each individual were obtained by spirometry and 6 minute walk distance. Enzyme activity (EA) in saliva samples from each individual was evaluated in vitro using fluorescent substrates and synthetic inhibitors of metalloproteinases (EDTA) and serine proteinases (PMSF). There was no significant difference between patients with COPD and healthy relationship with the AE or total inhibition of metalloproteinases and serine proteinases in saliva. Analysis of correlations between variables Person of the clinical evaluation of COPD and the AE tests showed that the FEV1 correlated negatively to total EA (p=0.03, r =-0.473) and inhibition of metalloproteinases (p=0.02, r =-0.498). There was no correlation between the parameters evaluated in the control group. These results open perspectives for studies of enzyme kinetics in saliva, using specific substrates for enzymes most representative during the progression of COPD.
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spelling Silva, Carlos AlbertoCPF:00000000000CPF:33086068876http://lattes.cnpq.br/0510261866037537Basso, Juliana Gerunda2015-04-22T17:20:22Z2010-04-142009-12-18BASSO, Juliana Gerunda. Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with pulmonary illness obstrutiva chronicle.. 2009. 71 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2009.http://bibliotecatede.uninove.br/tede/handle/tede/811The present study was conducted to evaluate the activity of enzymatic components of saliva and possible correlations with clinical parameters in patients with COPD. The sample consisted of 30 individuals, 20 individuals with COPD and 10 controls. The clinical parameters of each individual were obtained by spirometry and 6 minute walk distance. Enzyme activity (EA) in saliva samples from each individual was evaluated in vitro using fluorescent substrates and synthetic inhibitors of metalloproteinases (EDTA) and serine proteinases (PMSF). There was no significant difference between patients with COPD and healthy relationship with the AE or total inhibition of metalloproteinases and serine proteinases in saliva. Analysis of correlations between variables Person of the clinical evaluation of COPD and the AE tests showed that the FEV1 correlated negatively to total EA (p=0.03, r =-0.473) and inhibition of metalloproteinases (p=0.02, r =-0.498). There was no correlation between the parameters evaluated in the control group. These results open perspectives for studies of enzyme kinetics in saliva, using specific substrates for enzymes most representative during the progression of COPD.O presente estudo teve como objetivo avaliar a atividade dos componentes enzim??ticos da saliva e as poss??veis correla????es com os par??metros de avalia????o cl??nica em pacientes com DPOC. A amostra foi composta por 30 indiv??duos, sendo 20 indiv??duos do grupo DPOC e 10 do grupo saud??vel. Os par??metros cl??nicos de cada indiv??duo foram obtidos por espirometria e teste da caminhada dos 6 minutos. A atividade enzim??tica (AE) nas amostras individuais de saliva de cada indiv??duo foi avaliada in vitro, utilizando substratos fluorescentes com apagamento intramolecular e inibidores da atividade metaloproteinases (EDTA) e serinoproteinases (PMSF). N??o se observou diferen??as significantes entre os pacientes com DPOC e saud??veis com rela????o a AE total ou inibi????o de metaloproteinases e serinoproteinases na saliva. As an??lises de correla????es entre as vari??veis da avalia????o cl??nica do grupo DPOC e os ensaios de AE, demonstraram que o FEV1 apresentou correla????o negativa em rela????o a AE total (p=0,03, r=-0,473) e a inibi????o de metaloproteinases (p=0,02, r=-0,498). N??o se observou correla????es entre os par??metros avaliados no grupo controle. Esses resultados abrem novas perspectivas para os estudos de cin??tica enzim??tica na saliva, utilizando substratos espec??ficos para as enzimas mais representativas durante a progress??o da DPOC.Made available in DSpace on 2015-04-22T17:20:22Z (GMT). No. of bitstreams: 1 Juliana Gerunda Basso.pdf: 6891293 bytes, checksum: 6dc72b08987567f73599f010c3c3e599 (MD5) Previous issue date: 2009-12-18application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??deDoen??a pulmonar obstrutiva cr??nicaMetaloproteinasesSerinoproteinases e saliva.Pulmonary illness obstrutiva chronicleMetaloproteinasesSerinoproteinases and saliva.CIENCIAS DA SAUDEAvalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with Pulmonary Illness Obstrutiva Chronicle (DPOC)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALJuliana Gerunda Basso.pdfapplication/pdf6891293http://localhost:8080/tede/bitstream/tede/811/1/Juliana+Gerunda+Basso.pdf6dc72b08987567f73599f010c3c3e599MD51tede/8112018-06-15 12:30:05.296oai:localhost:tede/811Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:30:05Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
dc.title.alternative.eng.fl_str_mv Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with Pulmonary Illness Obstrutiva Chronicle (DPOC)
title Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
spellingShingle Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
Basso, Juliana Gerunda
Doen??a pulmonar obstrutiva cr??nica
Metaloproteinases
Serinoproteinases e saliva.
Pulmonary illness obstrutiva chronicle
Metaloproteinases
Serinoproteinases and saliva.
CIENCIAS DA SAUDE
title_short Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
title_full Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
title_fullStr Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
title_full_unstemmed Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
title_sort Avalia????o da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)
author Basso, Juliana Gerunda
author_facet Basso, Juliana Gerunda
author_role author
dc.contributor.advisor1.fl_str_mv Silva, Carlos Alberto
dc.contributor.advisor1ID.fl_str_mv CPF:00000000000
dc.contributor.authorID.fl_str_mv CPF:33086068876
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0510261866037537
dc.contributor.author.fl_str_mv Basso, Juliana Gerunda
contributor_str_mv Silva, Carlos Alberto
dc.subject.por.fl_str_mv Doen??a pulmonar obstrutiva cr??nica
Metaloproteinases
Serinoproteinases e saliva.
topic Doen??a pulmonar obstrutiva cr??nica
Metaloproteinases
Serinoproteinases e saliva.
Pulmonary illness obstrutiva chronicle
Metaloproteinases
Serinoproteinases and saliva.
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv Pulmonary illness obstrutiva chronicle
Metaloproteinases
Serinoproteinases and saliva.
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The present study was conducted to evaluate the activity of enzymatic components of saliva and possible correlations with clinical parameters in patients with COPD. The sample consisted of 30 individuals, 20 individuals with COPD and 10 controls. The clinical parameters of each individual were obtained by spirometry and 6 minute walk distance. Enzyme activity (EA) in saliva samples from each individual was evaluated in vitro using fluorescent substrates and synthetic inhibitors of metalloproteinases (EDTA) and serine proteinases (PMSF). There was no significant difference between patients with COPD and healthy relationship with the AE or total inhibition of metalloproteinases and serine proteinases in saliva. Analysis of correlations between variables Person of the clinical evaluation of COPD and the AE tests showed that the FEV1 correlated negatively to total EA (p=0.03, r =-0.473) and inhibition of metalloproteinases (p=0.02, r =-0.498). There was no correlation between the parameters evaluated in the control group. These results open perspectives for studies of enzyme kinetics in saliva, using specific substrates for enzymes most representative during the progression of COPD.
publishDate 2009
dc.date.issued.fl_str_mv 2009-12-18
dc.date.available.fl_str_mv 2010-04-14
dc.date.accessioned.fl_str_mv 2015-04-22T17:20:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BASSO, Juliana Gerunda. Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with pulmonary illness obstrutiva chronicle.. 2009. 71 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2009.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/tede/handle/tede/811
identifier_str_mv BASSO, Juliana Gerunda. Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with pulmonary illness obstrutiva chronicle.. 2009. 71 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2009.
url http://bibliotecatede.uninove.br/tede/handle/tede/811
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Universidade Nove de Julho
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