Avaliação da resposta tecidual a discos de ácido poli-l/d láctico: estudo experimental em ovelhas

• Autor(a) principal: Cielo, Vinícius Flores
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional PUCRS
• Texto Completo: http://hdl.handle.net/10923/405
• topic: ODONTOLOGIA; CIRURGIA BUCOMAXILOFACIAL; TRAUMATOLOGIA BUCOMAXILOFACIAL; CICATRIZAÇÃO (ODONTOLOGIA); MATERIAIS BIOCOMPATÍVEIS; ÁCIDO POLIGLICÓLICO; OSSOS - CIRURGIA

The aim of this study was to evaluate the inflammatory reaction, more specifically the foreign body reaction and behavior of bone remodeling in sheep’s tibiae and ulnae after installation of copolymer discs consisting of 95% poly-L lactic acid and 5% acid poly-D Lactic (PLDLA). It was used two adult female sheep which was fixed, by screws, for circular discs of PLDLA in each tibia and ulna of the sheep. A total of 16 discs were fixed in each animal. The sheeps were randomly divided into two groups according to the time of death after surgery: 30 and 60 days. Subsequently the samples were processed, resulting in a slide for each sample. After that the slides were investigated in optical microscope with magnification of 40 x and 100 x. In the slices of periosteal were evaluated the inflammatory infiltrate in a descriptive way in an increase of 40 X and quantitatively, by counting inflammatory cells in five random fields per sample in an optical microscope in an increase of 100 X. The inflammatory density was quantified according to the following scale: grade 0 - absence of inflammatory infiltrate, grade 1 - less than 30 cells per field, grade 2 - 30 to 100 cells per field and grade 3 - greater than 100 cells per field. In bone tissue it was analyzed two random fields per sample and if some bone resorption area appeared in the area of contact with the discs, this was described. In the end of the study, histological sections of the both groups showed tissue composed of organized fibroblasts and the inflammatory infiltrate varied from absent to mild. Talking about bone resorption, it had association with compression of the discs to the bone tissue, without evidence of bone resorption caused by the discs. At the end of the study verified biocompatibility, abscense of osteolisys at macroscopic view, disc structure undefformed and appropriated fit between titanium screw and PLDLA disc.