Effects of a whey protein concentrate and it’s peptides in the protection of ulcerative lesions at rat gastric mucosa

• Autor(a) principal: Bertoldo PACHECO, Maria Teresa
• Data de Publicação: 2023
• Outros Autores: BIGHETTI, Érica, ANTÔNIO, Márcia, de CARVALHO, João Ernesto, Filla ROSANELI, Caroline, SGARBIERI, Valdemiro Carlos
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9794
• topic: protein hydrolysates; peptides; rats, Wistar; stomach ulcer; hidrolisados de proteínas; peptideos; ratos wistar; úlcera gástrica

ObjectiveTo assess the ability of bovine whey protein hydrolysate and its low molecular weight fraction (molecularweight ≤1kDa) to protect the gastric mucosa of rats against ulcerative process induced by three differentagents. MethodsAdult Wistar rats were subjected to the indomethacin-induced ulcer (30mg/kg body weigh), absolute ethanol(1ml/animal) and immobilization and cold stress (40 C/2h), models. ResultsWhey protein hydrolysate was obtained by treatment with pancreatin to a degree of hydrolysis of 20% andfractionated using a tangential flow membrane with a molecular weight cut-off of 1kDa to obtain the fractioncontaining low molecular weight peptides (≤1kDa). In the ethanol-induced acute ulcer model (single dose),whey protein hydrolysate inhibited the gastric lesion index by 65.5% and the double dose resulted in a 77.4%inhibition. ConclusionFor the anti-inflammatory model, the cytoprotective effect of low molecular weight peptides was strongerthan that of total hydrolysate (53.1 and 71.6%, ulcerative lesion index) for single and double dose, respectively.No mucosa cytoprotective activity was found for whey protein concentrate, whey protein hydrolysate or WPHPin the immobilization and cold stress model.