Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus

• Autor(a) principal: Barreto da COSTA, Thacianna
• Data de Publicação: 2023
• Outros Autores: Gomes de MORAIS, Natália, Miranda de ALMEIDA, Thays, Santos SEVERO, Maiara, Machado Barbosa de CASTRO, Célia Maria
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9301
• topic: Cytokines; Malnutrition; Lymphocytes; Macrophages; Methicillin; Staphylococcus aureus; Citocinas; Desnutrição; Linfócitos; Macrófagos; Meticilina; Staphylococcus aureus

ObjectiveThe present study assessed the influence of neonatal malnutrition on the production of interferon gamma, interleukin-12 and interleukin-10 in cultured macrophages and lymphocytes infected in vitro with methicillin-sensitive or methicillin-resistant Staphylococcus aureus. MethodsMale Wistar rats were suckled by mothers fed during lactation a chow containing 17% protein for the nourished group and 8% for the undernourished group. After weaning, both groups received a normal diet in terms of protein content. The macrophages were obtained by bronchoalveolar lavage after tracheostomy. Cardiac puncture was used for the collection of lymphocytes. After isolation of different cell types, the challenge with the different strains was performed. Cytokines were measured by enzyme-linked immunosorbent assay (ELISA), using samples collected from the culture supernatant after an incubation period of 24 hours. ResultsMalnutrition let to slow weight gain, low interferon gamma in cultured alveolar macrophages and lymphocytes and low production of interleukin-12 in cultured alveolar macrophages. Only interferon gamma and interleukin-10 in cultured alveolar macrophages differed between the two groups and study strains. ConclusionThe neonatal malnutrition model used impaired weight gain and reduced production of proinflammatory cytokines (interleukin-12 and interferon gamma), indicating that protein malnutrition may result in an inability to fight infections. The methicillin-resistant Staphylococcus aureus strain stimulated macrophages to produce interferon gamma and interleukin-10, suggesting that this strain better provokes the immune system, specifically for this cell type.