Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/9301 |
Resumo: | ObjectiveThe present study assessed the influence of neonatal malnutrition on the production of interferon gamma, interleukin-12 and interleukin-10 in cultured macrophages and lymphocytes infected in vitro with methicillin-sensitive or methicillin-resistant Staphylococcus aureus. MethodsMale Wistar rats were suckled by mothers fed during lactation a chow containing 17% protein for the nourished group and 8% for the undernourished group. After weaning, both groups received a normal diet in terms of protein content. The macrophages were obtained by bronchoalveolar lavage after tracheostomy. Cardiac puncture was used for the collection of lymphocytes. After isolation of different cell types, the challenge with the different strains was performed. Cytokines were measured by enzyme-linked immunosorbent assay (ELISA), using samples collected from the culture supernatant after an incubation period of 24 hours. ResultsMalnutrition let to slow weight gain, low interferon gamma in cultured alveolar macrophages and lymphocytes and low production of interleukin-12 in cultured alveolar macrophages. Only interferon gamma and interleukin-10 in cultured alveolar macrophages differed between the two groups and study strains. ConclusionThe neonatal malnutrition model used impaired weight gain and reduced production of proinflammatory cytokines (interleukin-12 and interferon gamma), indicating that protein malnutrition may result in an inability to fight infections. The methicillin-resistant Staphylococcus aureus strain stimulated macrophages to produce interferon gamma and interleukin-10, suggesting that this strain better provokes the immune system, specifically for this cell type. |
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Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureusDesnutrição neonatal e produção de IFN-J, IL-12 e IL-10 por macrófagos/linfócitos: estudo da infecção celular, in vitro, por Staphylococcus aureus meticilina sensível e meticilina resistenteCytokinesMalnutritionLymphocytesMacrophagesMethicillinStaphylococcus aureusCitocinasDesnutriçãoLinfócitosMacrófagosMeticilinaStaphylococcus aureusObjectiveThe present study assessed the influence of neonatal malnutrition on the production of interferon gamma, interleukin-12 and interleukin-10 in cultured macrophages and lymphocytes infected in vitro with methicillin-sensitive or methicillin-resistant Staphylococcus aureus. MethodsMale Wistar rats were suckled by mothers fed during lactation a chow containing 17% protein for the nourished group and 8% for the undernourished group. After weaning, both groups received a normal diet in terms of protein content. The macrophages were obtained by bronchoalveolar lavage after tracheostomy. Cardiac puncture was used for the collection of lymphocytes. After isolation of different cell types, the challenge with the different strains was performed. Cytokines were measured by enzyme-linked immunosorbent assay (ELISA), using samples collected from the culture supernatant after an incubation period of 24 hours. ResultsMalnutrition let to slow weight gain, low interferon gamma in cultured alveolar macrophages and lymphocytes and low production of interleukin-12 in cultured alveolar macrophages. Only interferon gamma and interleukin-10 in cultured alveolar macrophages differed between the two groups and study strains. ConclusionThe neonatal malnutrition model used impaired weight gain and reduced production of proinflammatory cytokines (interleukin-12 and interferon gamma), indicating that protein malnutrition may result in an inability to fight infections. The methicillin-resistant Staphylococcus aureus strain stimulated macrophages to produce interferon gamma and interleukin-10, suggesting that this strain better provokes the immune system, specifically for this cell type.ObjetivoAvaliar a influência da desnutrição neonatal sobre a produção de Interferon gama, Interleucina-12 e Interleucina-10 em cultura de macrófagos alveolares e linfócitos infectados, in vitro, com Staphylococcus aureus sensível/resistente à meticilina. MétodosRatos machos Wistar foram amamentados por mães cuja dieta, durante a lactação, continha 17% de proteína no grupo nutrido e 8% no grupo desnutrido. Após desmame, ambos os grupos receberam a dieta normoproteica. Os macrófagos foram obtidos após traqueostomia, através da coleta do lavado broncoalveolar. Para obtenção dos linfócitos, foi realizado o procedimento cirúrgico de punção cardíaca. Após o isolamento dos diferentes tipos celulares, procedeu-se à realização dos estímulos com as cepas de estudo. A dosagem das citocinas foi realizada pelo método de Enzyme-Linked Immunosorbent Assay, a partir de amostras coletadas do sobrenadante das culturas após 24 horas de incubação. ResultadosA desnutrição acarretou diminuição do crescimento ponderal, redução na produção de Interferon gama em cultura de macrófagos alveolares e linfócitos e diminuição na produção de Interleucina-12 em cultura de macrófagos alveolares. Apenas a produção de Interferon gama e Interleucina-10 em cultura de macrófagos alveolares apresentou diferença entre as cepas analisadas, em ambos os grupos estudados. ConclusãoO modelo de desnutrição neonatal produziu sequela no peso corporal e reduziu a produção de citocinas pró-inflamatórias (Interleucina-12 e Interferon gama), indicando que esse modelo de desnutrição pode comprometer a resolução de um processo infeccioso. A cepa de Staphylococcus aureus resistente à meticilina estimulou uma maior produção de Interferon gama e Interleucina-10 por macrófagos alveolares, o que sugeriu estimulação imunológica mais intensa, por essa cepa, nesse tipo celular especificamente.Núcleo de Editoração – PUC-Campinas2023-08-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9301Brazilian Journal of Nutrition; Vol. 25 No. 5 (2012): Revista de NutriçãoRevista de Nutrição; Vol. 25 Núm. 5 (2012): Revista de NutriçãoRevista de Nutrição; v. 25 n. 5 (2012): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9301/6678Copyright (c) 2023 Thacianna Barreto da COSTA, Natália Gomes de MORAIS, Thays Miranda de ALMEIDA, Maiara Santos SEVERO, Célia Maria Machado Barbosa de CASTROhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBarreto da COSTA, Thacianna Gomes de MORAIS, Natália Miranda de ALMEIDA, Thays Santos SEVERO, MaiaraMachado Barbosa de CASTRO, Célia Maria 2023-10-05T19:07:57Zoai:ojs.periodicos.puc-campinas.edu.br:article/9301Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-10-05T19:07:57Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus Desnutrição neonatal e produção de IFN-J, IL-12 e IL-10 por macrófagos/linfócitos: estudo da infecção celular, in vitro, por Staphylococcus aureus meticilina sensível e meticilina resistente |
title |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus |
spellingShingle |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus Barreto da COSTA, Thacianna Cytokines Malnutrition Lymphocytes Macrophages Methicillin Staphylococcus aureus Citocinas Desnutrição Linfócitos Macrófagos Meticilina Staphylococcus aureus |
title_short |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus |
title_full |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus |
title_fullStr |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus |
title_full_unstemmed |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus |
title_sort |
Early malnutrition and production of IFN-J, IL-12 and IL-10 by macrophages/lymphocytes: in vitro study of cell infection by methicillin-sensitive and methicillin-resistant Staphylococcus aureus |
author |
Barreto da COSTA, Thacianna |
author_facet |
Barreto da COSTA, Thacianna Gomes de MORAIS, Natália Miranda de ALMEIDA, Thays Santos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria |
author_role |
author |
author2 |
Gomes de MORAIS, Natália Miranda de ALMEIDA, Thays Santos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Barreto da COSTA, Thacianna Gomes de MORAIS, Natália Miranda de ALMEIDA, Thays Santos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria |
dc.subject.por.fl_str_mv |
Cytokines Malnutrition Lymphocytes Macrophages Methicillin Staphylococcus aureus Citocinas Desnutrição Linfócitos Macrófagos Meticilina Staphylococcus aureus |
topic |
Cytokines Malnutrition Lymphocytes Macrophages Methicillin Staphylococcus aureus Citocinas Desnutrição Linfócitos Macrófagos Meticilina Staphylococcus aureus |
description |
ObjectiveThe present study assessed the influence of neonatal malnutrition on the production of interferon gamma, interleukin-12 and interleukin-10 in cultured macrophages and lymphocytes infected in vitro with methicillin-sensitive or methicillin-resistant Staphylococcus aureus. MethodsMale Wistar rats were suckled by mothers fed during lactation a chow containing 17% protein for the nourished group and 8% for the undernourished group. After weaning, both groups received a normal diet in terms of protein content. The macrophages were obtained by bronchoalveolar lavage after tracheostomy. Cardiac puncture was used for the collection of lymphocytes. After isolation of different cell types, the challenge with the different strains was performed. Cytokines were measured by enzyme-linked immunosorbent assay (ELISA), using samples collected from the culture supernatant after an incubation period of 24 hours. ResultsMalnutrition let to slow weight gain, low interferon gamma in cultured alveolar macrophages and lymphocytes and low production of interleukin-12 in cultured alveolar macrophages. Only interferon gamma and interleukin-10 in cultured alveolar macrophages differed between the two groups and study strains. ConclusionThe neonatal malnutrition model used impaired weight gain and reduced production of proinflammatory cytokines (interleukin-12 and interferon gamma), indicating that protein malnutrition may result in an inability to fight infections. The methicillin-resistant Staphylococcus aureus strain stimulated macrophages to produce interferon gamma and interleukin-10, suggesting that this strain better provokes the immune system, specifically for this cell type. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9301 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9301 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9301/6678 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 25 No. 5 (2012): Revista de Nutrição Revista de Nutrição; Vol. 25 Núm. 5 (2012): Revista de Nutrição Revista de Nutrição; v. 25 n. 5 (2012): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
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1799126072555470848 |