Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/8346 |
Resumo: | ObjectiveTo assess microbicide function and macrophage viability after in vitro cellular infection by methicillin-sensitive/resistant Staphylococcus aureus in nourished rats and rats subjected to neonatal malnutrition. MethodsMale Wistar rats (n=40) were divided in two groups: Nourished (rats suckled by dams consuming a 17% casein diet) and Malnourished (rats suckled by dams consuming an 8% casein diet). Macrophages were recovered after tracheotomy, by bronchoalveolar lavage. After mononuclear cell isolation, four systems were established: negative control composed exclusively of phagocytes; positive control composed of macrophages pluslipopolysaccharide; and two testing systems, macrophages plus methicillin-sensitive Staphylococcus aureus and macrophages plus methicillin-resistant Staphylococcus aureus. The plates were incubated in a humid atmosphere at 37 degrees Celsius containing 5% CO2 for 24 hours. After this period tests the microbicidalresponse, cytokine production, and cell viability were analyzed. The statistical analysis consisted of analysis of variance (p<0.05). ResultsMalnutrition reduced weight gain, rate of phagocytosis, production of superoxide anion and nitric oxide, and macrophage viability. Production of nitrite and interleukin 18, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus were lower. ConclusionThe neonatal malnutrition model compromised phagocyte function and reduced microbicidal response and cell viability. Interaction between malnutrition and the methicillin-resistant strain decreased the production of inflammatory mediators by effector cells of the immune response, which may compromise the immune system’s defense ability. |
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Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillinEfeitos tardios da desnutrição neonatal na resposta microbicida, produção de citocinas e viabilidade de macrófagos na infecção por Staphylococcus aureus sensível/resistente a meticilinaMacrophagesMalnutritionMethicillinStaphylococcusMacrófagosDesnutriçãoMeticilinaStaphylococcusObjectiveTo assess microbicide function and macrophage viability after in vitro cellular infection by methicillin-sensitive/resistant Staphylococcus aureus in nourished rats and rats subjected to neonatal malnutrition. MethodsMale Wistar rats (n=40) were divided in two groups: Nourished (rats suckled by dams consuming a 17% casein diet) and Malnourished (rats suckled by dams consuming an 8% casein diet). Macrophages were recovered after tracheotomy, by bronchoalveolar lavage. After mononuclear cell isolation, four systems were established: negative control composed exclusively of phagocytes; positive control composed of macrophages pluslipopolysaccharide; and two testing systems, macrophages plus methicillin-sensitive Staphylococcus aureus and macrophages plus methicillin-resistant Staphylococcus aureus. The plates were incubated in a humid atmosphere at 37 degrees Celsius containing 5% CO2 for 24 hours. After this period tests the microbicidalresponse, cytokine production, and cell viability were analyzed. The statistical analysis consisted of analysis of variance (p<0.05). ResultsMalnutrition reduced weight gain, rate of phagocytosis, production of superoxide anion and nitric oxide, and macrophage viability. Production of nitrite and interleukin 18, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus were lower. ConclusionThe neonatal malnutrition model compromised phagocyte function and reduced microbicidal response and cell viability. Interaction between malnutrition and the methicillin-resistant strain decreased the production of inflammatory mediators by effector cells of the immune response, which may compromise the immune system’s defense ability.ObjetivoAvaliar a função microbicida e a viabilidade de macrófagos, após infecção celular in vitro, com Staphylococcus aureus sensível/resistente a meticilina, em ratos nutridos ou submetidos a desnutrição neonatal. MétodosRatos machos Wistar (n=40) foram divididos em dois grupos distintos: Nutrido (ratos amamentados por mães submetidas a dieta com 17% de caseína) e Desnutrido (ratos amamentados por mães submetidas a dieta com 8% de caseína). Os macrófagos foram recuperados após procedimento cirúrgico de traqueostomia, através da coleta do lavado broncoalveolar. Após o isolamento dos mononucleares, foram estabelecidos quatro sistemas: controle negativo, composto apenas pelos fagócitos; controle positivo, macrófagos mais lipopolissacarídeo; e dois sistemas teste, macrófagos mais Staphylococcus aureus sensível e resistente a meticilina. As placas foram incubadas por 24 horas, à temperatura de 37ºC, com atmosfera úmida e 5% de dióxido de carbono. Transcorrido esse período, foram realizados ensaios para análise da resposta microbicida, produção de citocinas e viabilidade celular. Na análise estatística, utilizou-se analysis of variance, admitindo-se p<0,05. ResultadosA desnutrição acarretou redução do crescimento ponderal dos animais, da taxa de fagocitose, da produção de óxido nítrico, do ânion superóxido e da viabilidade de macrófagos. Houve menor produção de nitrito, de interleucina 18 e da viabilidade dos macrófagos infectados com Staphylococcus aureus meticilina-resistente. ConclusãoO modelo de desnutrição neonatal adotado comprometeu a função dos fagócitos, com redução da resposta microbicida e da viabilidade celular. A interação de desnutrição com cepa resistente induziu baixa produção de mediadores inflamatórios por células efetoras da resposta imunológica, o que poderá resultar em comprometimento da defesa. Núcleo de Editoração – PUC-Campinas2023-04-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/8346Brazilian Journal of Nutrition; Vol. 27 No. 5 (2014): Revista de NutriçãoRevista de Nutrição; Vol. 27 Núm. 5 (2014): Revista de NutriçãoRevista de Nutrição; v. 27 n. 5 (2014): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPenghttps://periodicos.puc-campinas.edu.br/nutricao/article/view/8346/5868Copyright (c) 2023 Natália Gomes de MORAIS, Thacianna Barreto da COSTA, Maiara Santos SEVERO, Célia Maria Machado Barbosa de CASTROhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGomes de MORAIS, Natália Barreto da COSTA, ThaciannaSantos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria 2023-04-14T18:40:26Zoai:ojs.periodicos.puc-campinas.edu.br:article/8346Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-04-14T18:40:26Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin Efeitos tardios da desnutrição neonatal na resposta microbicida, produção de citocinas e viabilidade de macrófagos na infecção por Staphylococcus aureus sensível/resistente a meticilina |
title |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin |
spellingShingle |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin Gomes de MORAIS, Natália Macrophages Malnutrition Methicillin Staphylococcus Macrófagos Desnutrição Meticilina Staphylococcus |
title_short |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin |
title_full |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin |
title_fullStr |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin |
title_full_unstemmed |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin |
title_sort |
Long-term effects of neonatal malnutrition on microbicide response, production of cytokines, and survival of macrophages infected by Staphylococcus aureus sensitive/resistant to methicillin |
author |
Gomes de MORAIS, Natália |
author_facet |
Gomes de MORAIS, Natália Barreto da COSTA, Thacianna Santos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria |
author_role |
author |
author2 |
Barreto da COSTA, Thacianna Santos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Gomes de MORAIS, Natália Barreto da COSTA, Thacianna Santos SEVERO, Maiara Machado Barbosa de CASTRO, Célia Maria |
dc.subject.por.fl_str_mv |
Macrophages Malnutrition Methicillin Staphylococcus Macrófagos Desnutrição Meticilina Staphylococcus |
topic |
Macrophages Malnutrition Methicillin Staphylococcus Macrófagos Desnutrição Meticilina Staphylococcus |
description |
ObjectiveTo assess microbicide function and macrophage viability after in vitro cellular infection by methicillin-sensitive/resistant Staphylococcus aureus in nourished rats and rats subjected to neonatal malnutrition. MethodsMale Wistar rats (n=40) were divided in two groups: Nourished (rats suckled by dams consuming a 17% casein diet) and Malnourished (rats suckled by dams consuming an 8% casein diet). Macrophages were recovered after tracheotomy, by bronchoalveolar lavage. After mononuclear cell isolation, four systems were established: negative control composed exclusively of phagocytes; positive control composed of macrophages pluslipopolysaccharide; and two testing systems, macrophages plus methicillin-sensitive Staphylococcus aureus and macrophages plus methicillin-resistant Staphylococcus aureus. The plates were incubated in a humid atmosphere at 37 degrees Celsius containing 5% CO2 for 24 hours. After this period tests the microbicidalresponse, cytokine production, and cell viability were analyzed. The statistical analysis consisted of analysis of variance (p<0.05). ResultsMalnutrition reduced weight gain, rate of phagocytosis, production of superoxide anion and nitric oxide, and macrophage viability. Production of nitrite and interleukin 18, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus were lower. ConclusionThe neonatal malnutrition model compromised phagocyte function and reduced microbicidal response and cell viability. Interaction between malnutrition and the methicillin-resistant strain decreased the production of inflammatory mediators by effector cells of the immune response, which may compromise the immune system’s defense ability. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8346 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8346 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8346/5868 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 27 No. 5 (2014): Revista de Nutrição Revista de Nutrição; Vol. 27 Núm. 5 (2014): Revista de Nutrição Revista de Nutrição; v. 27 n. 5 (2014): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
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1799126069700198400 |