Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista odonto ciência (Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588 |
Resumo: | Purpose: To assess the association between the polymorphism in exon-1 of the MBL2 gene and the periodontal disease in type 2 diabetic patients. Methods: The sample comprised of 100 patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth the probing depth (PD), bleeding on probing (BOP), clinical attachment loss (CAL), plaque index (PI) and the number of teeth present. Periodontal disease was defined as at least four sites with loss of attachment of ≥5 mm, with one or more of those sites having a pocket of ≥ 4 mm. The collection of scaling cells from the oral mucosa was carried out and the detection of MBL2 polymorphism was made by real time PCR and melting temperature curve analysis. Results: In a type 2 diabetic population, no significant statistical differences in MBL2 polymorphisms genotype or allele frequencies were observed among subjects with periodontal disease. Conclusion: This study indicates that the polymorphisms in exon-1 of the MBL2 gene are not related to periodontal disease in a type 2 diabetic population. |
id |
PUC_RS-22_3442bb69b731515b84652a38f49e05a3 |
---|---|
oai_identifier_str |
oai:ojs.revistaseletronicas.pucrs.br:article/9588 |
network_acronym_str |
PUC_RS-22 |
network_name_str |
Revista odonto ciência (Online) |
repository_id_str |
|
spelling |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patientsPolimorfimos da lectina de ligação da manose (MBL2) e doença periodontal em pacientes diabéticosPolymorphismperiodontitisDiabetes MellitusPolimorfismoperiodontiteDiabetes mellitusPurpose: To assess the association between the polymorphism in exon-1 of the MBL2 gene and the periodontal disease in type 2 diabetic patients. Methods: The sample comprised of 100 patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth the probing depth (PD), bleeding on probing (BOP), clinical attachment loss (CAL), plaque index (PI) and the number of teeth present. Periodontal disease was defined as at least four sites with loss of attachment of ≥5 mm, with one or more of those sites having a pocket of ≥ 4 mm. The collection of scaling cells from the oral mucosa was carried out and the detection of MBL2 polymorphism was made by real time PCR and melting temperature curve analysis. Results: In a type 2 diabetic population, no significant statistical differences in MBL2 polymorphisms genotype or allele frequencies were observed among subjects with periodontal disease. Conclusion: This study indicates that the polymorphisms in exon-1 of the MBL2 gene are not related to periodontal disease in a type 2 diabetic population.Objetivo: Avaliar a associação entre o polimorfismo no exon-1 do gene MBL2 e a doença periodontal em pacientes diabéticos tipo 2. Método: A amostra foi composta por 100 pacientes que foram submetidos a um exame clínico periodontal que avaliou seis sítios por dente a profundidade de sondagem (PS), sangramento à sondagem (SS), perda de inserção clínica (PIC), índice de placa (IP) e o número de dentes presente. A doença periodontal foi definida como pelo menos quatro sítios com perda de inserção de ≥5mm, com um ou mais destes sítios tendo uma bolsa de ≥4mm. Foram coletadas células de descamação da mucosa oral e a detecção do polimorfismo foi feita através da PCR em tempo real e análise da temperatura de melting. Resultados: Em uma população de diabéticos tipo 2, não houve diferenças estatisticamente significantes nos genótipos do polimorfismo da MBL2 ou freqüência alélica observadas entre os indivíduos com doença periodontal. Conclusão: Este estudo indicou que o polimorfimos no exon-1 do gene da MBL2 não foi relacionado à doença periodontal em uma população de diabéticos tipo 2.EDIPUCRS - Editora Universitária da PUCRS2011-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588Revista Odonto Ciência; Vol. 26 No. 3 (2011); 203 - 208Revista Odonto Ciência; v. 26 n. 3 (2011); 203 - 2081980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588/6879Araújo, Natália CostaBello, Darcyla Maria de AguiarCrovella, SergioSouza, Paulo Roberto Eleutério deDonos, NikosCimões, Renatainfo:eu-repo/semantics/openAccess2013-11-11T11:52:27Zoai:ojs.revistaseletronicas.pucrs.br:article/9588Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T11:52:27Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients Polimorfimos da lectina de ligação da manose (MBL2) e doença periodontal em pacientes diabéticos |
title |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients |
spellingShingle |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients Araújo, Natália Costa Polymorphism periodontitis Diabetes Mellitus Polimorfismo periodontite Diabetes mellitus |
title_short |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients |
title_full |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients |
title_fullStr |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients |
title_full_unstemmed |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients |
title_sort |
Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients |
author |
Araújo, Natália Costa |
author_facet |
Araújo, Natália Costa Bello, Darcyla Maria de Aguiar Crovella, Sergio Souza, Paulo Roberto Eleutério de Donos, Nikos Cimões, Renata |
author_role |
author |
author2 |
Bello, Darcyla Maria de Aguiar Crovella, Sergio Souza, Paulo Roberto Eleutério de Donos, Nikos Cimões, Renata |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Natália Costa Bello, Darcyla Maria de Aguiar Crovella, Sergio Souza, Paulo Roberto Eleutério de Donos, Nikos Cimões, Renata |
dc.subject.por.fl_str_mv |
Polymorphism periodontitis Diabetes Mellitus Polimorfismo periodontite Diabetes mellitus |
topic |
Polymorphism periodontitis Diabetes Mellitus Polimorfismo periodontite Diabetes mellitus |
description |
Purpose: To assess the association between the polymorphism in exon-1 of the MBL2 gene and the periodontal disease in type 2 diabetic patients. Methods: The sample comprised of 100 patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth the probing depth (PD), bleeding on probing (BOP), clinical attachment loss (CAL), plaque index (PI) and the number of teeth present. Periodontal disease was defined as at least four sites with loss of attachment of ≥5 mm, with one or more of those sites having a pocket of ≥ 4 mm. The collection of scaling cells from the oral mucosa was carried out and the detection of MBL2 polymorphism was made by real time PCR and melting temperature curve analysis. Results: In a type 2 diabetic population, no significant statistical differences in MBL2 polymorphisms genotype or allele frequencies were observed among subjects with periodontal disease. Conclusion: This study indicates that the polymorphisms in exon-1 of the MBL2 gene are not related to periodontal disease in a type 2 diabetic population. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588 |
url |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588/6879 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
dc.source.none.fl_str_mv |
Revista Odonto Ciência; Vol. 26 No. 3 (2011); 203 - 208 Revista Odonto Ciência; v. 26 n. 3 (2011); 203 - 208 1980-6523 0102-9460 reponame:Revista odonto ciência (Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Revista odonto ciência (Online) |
collection |
Revista odonto ciência (Online) |
repository.name.fl_str_mv |
Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
||odontociencia@pucrs.br |
_version_ |
1754820875882856448 |