Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients

Detalhes bibliográficos
Autor(a) principal: Araújo, Natália Costa
Data de Publicação: 2011
Outros Autores: Bello, Darcyla Maria de Aguiar, Crovella, Sergio, Souza, Paulo Roberto Eleutério de, Donos, Nikos, Cimões, Renata
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588
Resumo: Purpose: To assess the association between the polymorphism in exon-1 of the MBL2 gene and the periodontal disease in type 2 diabetic patients. Methods: The sample comprised of 100 patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth the probing depth (PD), bleeding on probing (BOP), clinical attachment loss (CAL), plaque index (PI) and the number of teeth present. Periodontal disease was defined as at least four sites with loss of attachment of ≥5 mm, with one or more of those sites having a pocket of ≥ 4 mm. The collection of scaling cells from the oral mucosa was carried out and the detection of MBL2 polymorphism was made by real time PCR and melting temperature curve analysis. Results: In a type 2 diabetic population, no significant statistical differences in MBL2 polymorphisms genotype or allele frequencies were observed among subjects with periodontal disease. Conclusion: This study indicates that the polymorphisms in exon-1 of the MBL2 gene are not related to periodontal disease in a type 2 diabetic population.
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spelling Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patientsPolimorfimos da lectina de ligação da manose (MBL2) e doença periodontal em pacientes diabéticosPolymorphismperiodontitisDiabetes MellitusPolimorfismoperiodontiteDiabetes mellitusPurpose: To assess the association between the polymorphism in exon-1 of the MBL2 gene and the periodontal disease in type 2 diabetic patients. Methods: The sample comprised of 100 patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth the probing depth (PD), bleeding on probing (BOP), clinical attachment loss (CAL), plaque index (PI) and the number of teeth present. Periodontal disease was defined as at least four sites with loss of attachment of ≥5 mm, with one or more of those sites having a pocket of ≥ 4 mm. The collection of scaling cells from the oral mucosa was carried out and the detection of MBL2 polymorphism was made by real time PCR and melting temperature curve analysis. Results: In a type 2 diabetic population, no significant statistical differences in MBL2 polymorphisms genotype or allele frequencies were observed among subjects with periodontal disease. Conclusion: This study indicates that the polymorphisms in exon-1 of the MBL2 gene are not related to periodontal disease in a type 2 diabetic population.Objetivo: Avaliar a associação entre o polimorfismo no exon-1 do gene MBL2 e a doença periodontal em pacientes diabéticos tipo 2. Método: A amostra foi composta por 100 pacientes que foram submetidos a um exame clínico periodontal que avaliou seis sítios por dente a profundidade de sondagem (PS), sangramento à sondagem (SS), perda de inserção clínica (PIC), índice de placa (IP) e o número de dentes presente. A doença periodontal foi definida como pelo menos quatro sítios com perda de inserção de ≥5mm, com um ou mais destes sítios tendo uma bolsa de ≥4mm. Foram coletadas células de descamação da mucosa oral e a detecção do polimorfismo foi feita através da PCR em tempo real e análise da temperatura de melting. Resultados: Em uma população de diabéticos tipo 2, não houve diferenças estatisticamente significantes nos genótipos do polimorfismo da MBL2 ou freqüência alélica observadas entre os indivíduos com doença periodontal. Conclusão: Este estudo indicou que o polimorfimos no exon-1 do gene da MBL2 não foi relacionado à doença periodontal em uma população de diabéticos tipo 2.EDIPUCRS - Editora Universitária da PUCRS2011-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588Revista Odonto Ciência; Vol. 26 No. 3 (2011); 203 - 208Revista Odonto Ciência; v. 26 n. 3 (2011); 203 - 2081980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588/6879Araújo, Natália CostaBello, Darcyla Maria de AguiarCrovella, SergioSouza, Paulo Roberto Eleutério deDonos, NikosCimões, Renatainfo:eu-repo/semantics/openAccess2013-11-11T11:52:27Zoai:ojs.revistaseletronicas.pucrs.br:article/9588Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T11:52:27Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
Polimorfimos da lectina de ligação da manose (MBL2) e doença periodontal em pacientes diabéticos
title Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
spellingShingle Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
Araújo, Natália Costa
Polymorphism
periodontitis
Diabetes Mellitus
Polimorfismo
periodontite
Diabetes mellitus
title_short Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
title_full Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
title_fullStr Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
title_full_unstemmed Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
title_sort Mannose binding lectin genes (MBL2) polymorpshisms and the periodontal disease in diabetic patients
author Araújo, Natália Costa
author_facet Araújo, Natália Costa
Bello, Darcyla Maria de Aguiar
Crovella, Sergio
Souza, Paulo Roberto Eleutério de
Donos, Nikos
Cimões, Renata
author_role author
author2 Bello, Darcyla Maria de Aguiar
Crovella, Sergio
Souza, Paulo Roberto Eleutério de
Donos, Nikos
Cimões, Renata
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Araújo, Natália Costa
Bello, Darcyla Maria de Aguiar
Crovella, Sergio
Souza, Paulo Roberto Eleutério de
Donos, Nikos
Cimões, Renata
dc.subject.por.fl_str_mv Polymorphism
periodontitis
Diabetes Mellitus
Polimorfismo
periodontite
Diabetes mellitus
topic Polymorphism
periodontitis
Diabetes Mellitus
Polimorfismo
periodontite
Diabetes mellitus
description Purpose: To assess the association between the polymorphism in exon-1 of the MBL2 gene and the periodontal disease in type 2 diabetic patients. Methods: The sample comprised of 100 patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth the probing depth (PD), bleeding on probing (BOP), clinical attachment loss (CAL), plaque index (PI) and the number of teeth present. Periodontal disease was defined as at least four sites with loss of attachment of ≥5 mm, with one or more of those sites having a pocket of ≥ 4 mm. The collection of scaling cells from the oral mucosa was carried out and the detection of MBL2 polymorphism was made by real time PCR and melting temperature curve analysis. Results: In a type 2 diabetic population, no significant statistical differences in MBL2 polymorphisms genotype or allele frequencies were observed among subjects with periodontal disease. Conclusion: This study indicates that the polymorphisms in exon-1 of the MBL2 gene are not related to periodontal disease in a type 2 diabetic population.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/9588/6879
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 26 No. 3 (2011); 203 - 208
Revista Odonto Ciência; v. 26 n. 3 (2011); 203 - 208
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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