An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da PUC_RS |
Texto Completo: | http://tede2.pucrs.br/tede2/handle/tede/5498 |
Resumo: | Healthy aging is characterized by the ability of the immune system in preserve some of their functions intact, avoiding the appearance of some age-related diseases and thus contributing to reaching longevity. Furthermore, healthy aging is associated to a better stress coping. Studies have demonstrated immunological changes occurred in old individuals, as well as the consequences of stress on aging. However, few studies have addressed such changes in very old subjects. Here, we analyzed emotional factors and the changes in the lymphocytes subpopulations involved on aging, and the role of these changes to reach longevity healthily. A group of twenty healthy very old subjects together with a group of healthy old individuals and a groups of healthy young adults were recruited in this study. Stress levels were assessed by Perceived Stress Scale (PSS), while depression levels were investigated by Beck Depression Inventories II (BDI-II). Lymphocytes were isolated and assessed by flow cytometry. We observed some age-related changes in lymphocyte subpopulations. In particular, it was evidenced a similar CD4/CD8 ratio between very old subjects and young adults, with this rate being increased in old subjects. A significant increase in the percentage of Natural Killer cells (CD56+CD16+) was observed in the very old subjects, showing that the activity of these cells is well preserved in this group of subjects. Also, we noticed that very old subjects are less stressed, when compared to the other groups. Our data suggests that the combination between increasing and decreasing specific immune functions may generate a balance in the immune system, contributing to reach longevity healthily. |
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Bauer, Mois?s EvandroCPF:65934288091http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798647T5CPF:02409139060http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4490307U8Oliveira, ?gatha Schommer de2015-04-14T14:51:33Z2014-07-312014-03-25OLIVEIRA, ?gatha Schommer de. An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis. 2014. 46 f. Disserta??o (Mestrado em Biologia Celular e Molecular) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2014.http://tede2.pucrs.br/tede2/handle/tede/5498Healthy aging is characterized by the ability of the immune system in preserve some of their functions intact, avoiding the appearance of some age-related diseases and thus contributing to reaching longevity. Furthermore, healthy aging is associated to a better stress coping. Studies have demonstrated immunological changes occurred in old individuals, as well as the consequences of stress on aging. However, few studies have addressed such changes in very old subjects. Here, we analyzed emotional factors and the changes in the lymphocytes subpopulations involved on aging, and the role of these changes to reach longevity healthily. A group of twenty healthy very old subjects together with a group of healthy old individuals and a groups of healthy young adults were recruited in this study. Stress levels were assessed by Perceived Stress Scale (PSS), while depression levels were investigated by Beck Depression Inventories II (BDI-II). Lymphocytes were isolated and assessed by flow cytometry. We observed some age-related changes in lymphocyte subpopulations. In particular, it was evidenced a similar CD4/CD8 ratio between very old subjects and young adults, with this rate being increased in old subjects. A significant increase in the percentage of Natural Killer cells (CD56+CD16+) was observed in the very old subjects, showing that the activity of these cells is well preserved in this group of subjects. Also, we noticed that very old subjects are less stressed, when compared to the other groups. Our data suggests that the combination between increasing and decreasing specific immune functions may generate a balance in the immune system, contributing to reach longevity healthily.O envelhecimento bem sucedido ? caracterizado pela habilidade do sistema imune em manter algumas de suas fun??es intactas, evitando, assim, o aparecimento de doen?as relacionadas ? idade, e, portanto, contribuindo para o alcance da longevidade. Al?m disso, o envelhecimento bem sucedido tamb?m est? relacionado a uma melhor maneira de lidar com o estresse. Diversos estudos v?m demonstrando as altera??es imunol?gicas ocorridas em indiv?duos idosos, assim como as consequ?ncias do estresse para o envelhecimento. No entanto, poucos s?o os estudos que visam observar essas altera??es em indiv?duos longevos. Neste estudo, analisamos fatores ps?quicos e altera??es nos subtipos linfocit?rios envolvidos no envelhecimento e o papel destas altera??es para o alcance da longevidade de maneira saud?vel. Vinte longevos (86-99 anos), 15 idosos (60-80 anos) e 13 jovens adultos saud?veis (20-50 anos) foram recrutados neste estudo. Os n?veis de estresse foram avaliados pela Escala de Estresse Percebido (PSS), enquanto que os n?veis de depress?o foram investigados atrav?s do Invent?rio de Depress?o Beck II (BDI-II). As c?lulas mononucleares de sangue perif?rico foram isoladas e os subtipos linfocit?rios avaliados por citometria de fluxo. Dentre as popula??es avaliadas est?o as c?lulas T CD4+, T CD8+, B CD19+ e c?lulas NK CD56+. Observamos diversas altera??es idade dependente nos subtipos linfocit?rios. Em particular, foi evidenciada uma raz?o CD4/CD8 similar entre longevos e jovens, com um aumento desta raz?o ocorrendo em indiv?duos idosos. Um aumento significativo na percentagem das c?lulas Natural Killers (CD56+CD16+) foi observado nos longevos, sugerindo que a atividade destas c?lulas est? bem preservada nestes indiv?duos. Al?m disso, foi visto que os longevos s?o menos estressados, quando comparados aos outros grupos. Concluindo, nossos resultados sugerem que a combina??o entre o aumento e o decr?scimo de fun??es imunes espec?ficas pode gerar um balan?o no sistema imune, contribuindo para que se alcance a longevidade de forma saud?vel.Made available in DSpace on 2015-04-14T14:51:33Z (GMT). No. of bitstreams: 1 460008.pdf: 656276 bytes, checksum: f353cd5be78400bf9c6ccd1015321021 (MD5) Previous issue date: 2014-03-25application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/16637/460008.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Biologia Celular e MolecularPUCRSBRFaculdade de Bioci?nciasGERONTOLOGIA BIOM?DICABIOLOGIA MOLECULARBIOLOGIA CELULARIDOSOSLONGEVIDADEENVELHECIMENTO - ASPECTOS BIOL?GICOSCNPQ::CIENCIAS BIOLOGICASAn?lise de par?metros psiconeuroimunol?gicos em longevos saud?veisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis819824693009663736060060036528317262667714info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL460008.pdf.jpg460008.pdf.jpgimage/jpeg3453http://tede2.pucrs.br/tede2/bitstream/tede/5498/3/460008.pdf.jpga6b575cc2f5e9b909b577faf32a2738aMD53TEXT460008.pdf.txt460008.pdf.txttext/plain63246http://tede2.pucrs.br/tede2/bitstream/tede/5498/2/460008.pdf.txt16d654490cde637ff27b705824473857MD52ORIGINAL460008.pdfapplication/pdf656276http://tede2.pucrs.br/tede2/bitstream/tede/5498/1/460008.pdff353cd5be78400bf9c6ccd1015321021MD51tede/54982015-05-14 11:40:30.663oai:tede2.pucrs.br:tede/5498Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-05-14T14:40:30Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
title |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
spellingShingle |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis Oliveira, ?gatha Schommer de GERONTOLOGIA BIOM?DICA BIOLOGIA MOLECULAR BIOLOGIA CELULAR IDOSOS LONGEVIDADE ENVELHECIMENTO - ASPECTOS BIOL?GICOS CNPQ::CIENCIAS BIOLOGICAS |
title_short |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
title_full |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
title_fullStr |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
title_full_unstemmed |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
title_sort |
An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis |
author |
Oliveira, ?gatha Schommer de |
author_facet |
Oliveira, ?gatha Schommer de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bauer, Mois?s Evandro |
dc.contributor.advisor1ID.fl_str_mv |
CPF:65934288091 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798647T5 |
dc.contributor.authorID.fl_str_mv |
CPF:02409139060 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4490307U8 |
dc.contributor.author.fl_str_mv |
Oliveira, ?gatha Schommer de |
contributor_str_mv |
Bauer, Mois?s Evandro |
dc.subject.por.fl_str_mv |
GERONTOLOGIA BIOM?DICA BIOLOGIA MOLECULAR BIOLOGIA CELULAR IDOSOS LONGEVIDADE ENVELHECIMENTO - ASPECTOS BIOL?GICOS |
topic |
GERONTOLOGIA BIOM?DICA BIOLOGIA MOLECULAR BIOLOGIA CELULAR IDOSOS LONGEVIDADE ENVELHECIMENTO - ASPECTOS BIOL?GICOS CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
Healthy aging is characterized by the ability of the immune system in preserve some of their functions intact, avoiding the appearance of some age-related diseases and thus contributing to reaching longevity. Furthermore, healthy aging is associated to a better stress coping. Studies have demonstrated immunological changes occurred in old individuals, as well as the consequences of stress on aging. However, few studies have addressed such changes in very old subjects. Here, we analyzed emotional factors and the changes in the lymphocytes subpopulations involved on aging, and the role of these changes to reach longevity healthily. A group of twenty healthy very old subjects together with a group of healthy old individuals and a groups of healthy young adults were recruited in this study. Stress levels were assessed by Perceived Stress Scale (PSS), while depression levels were investigated by Beck Depression Inventories II (BDI-II). Lymphocytes were isolated and assessed by flow cytometry. We observed some age-related changes in lymphocyte subpopulations. In particular, it was evidenced a similar CD4/CD8 ratio between very old subjects and young adults, with this rate being increased in old subjects. A significant increase in the percentage of Natural Killer cells (CD56+CD16+) was observed in the very old subjects, showing that the activity of these cells is well preserved in this group of subjects. Also, we noticed that very old subjects are less stressed, when compared to the other groups. Our data suggests that the combination between increasing and decreasing specific immune functions may generate a balance in the immune system, contributing to reach longevity healthily. |
publishDate |
2014 |
dc.date.available.fl_str_mv |
2014-07-31 |
dc.date.issued.fl_str_mv |
2014-03-25 |
dc.date.accessioned.fl_str_mv |
2015-04-14T14:51:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, ?gatha Schommer de. An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis. 2014. 46 f. Disserta??o (Mestrado em Biologia Celular e Molecular) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2014. |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/5498 |
identifier_str_mv |
OLIVEIRA, ?gatha Schommer de. An?lise de par?metros psiconeuroimunol?gicos em longevos saud?veis. 2014. 46 f. Disserta??o (Mestrado em Biologia Celular e Molecular) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2014. |
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