Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5

Detalhes bibliográficos
Autor(a) principal: Jacinto, Raquel
Data de Publicação: 2021
Outros Autores: Sampaio, Pedro, Roxo-Rosa, Mónica, Pestana, Sara, Lopes, Susana S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116408
Resumo: Funding: This work was supported by the Fundação para a Ciência e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). SL is funded by an FCT CEEC contract for Principal Investigator. The work was also supported by Sociedade Portuguesa de Nefrologia (research grant 2015), iNOVA4Health – UID/Multi/04462/2013 (a program financially supported by Fundação para a Ciência e Tecnologia/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement) and Fundação para a Ciência e Tecnologia (research grant – PTDC/BEXBID/1411/2014). MR-R was supported by national funds through Fundação para a Ciência e Tecnologia, in the context of the celebration of the program contract foreseen in the numbers 4, 5, and 6 of article 23 of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July. Zebrafish used as animal model were reared and maintained in the CEDOC Fish Facility, with the support from the research consortia Congento, co-financed by Lisboa Regional Operational Program (Lisboa2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Fundação para a Ciência e Tecnologia
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spelling Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5cilia lengthdand5flow dynamicsleft-rightPkd2zebrafishDevelopmental BiologyCell BiologyFunding: This work was supported by the Fundação para a Ciência e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). SL is funded by an FCT CEEC contract for Principal Investigator. The work was also supported by Sociedade Portuguesa de Nefrologia (research grant 2015), iNOVA4Health – UID/Multi/04462/2013 (a program financially supported by Fundação para a Ciência e Tecnologia/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement) and Fundação para a Ciência e Tecnologia (research grant – PTDC/BEXBID/1411/2014). MR-R was supported by national funds through Fundação para a Ciência e Tecnologia, in the context of the celebration of the program contract foreseen in the numbers 4, 5, and 6 of article 23 of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July. Zebrafish used as animal model were reared and maintained in the CEDOC Fish Facility, with the support from the research consortia Congento, co-financed by Lisboa Regional Operational Program (Lisboa2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Fundação para a Ciência e TecnologiaThe left-right (LR) field recognizes the importance of the mechanism involving the calcium permeable channel Polycystin-2. However, whether the early LR symmetry breaking mechanism is exclusively via Polycystin-2 has not been tested. For that purpose, we need to be able to isolate the effects of decreasing the levels of Pkd2 protein from any eventual effects on flow dynamics. Here we demonstrate that curly-up (cup) homozygous mutants have abnormal flow dynamics. In addition, we performed one cell stage Pkd2 knockdowns and LR organizer specific Pkd2 knockdowns and observed that both techniques resulted in shorter cilia length and abnormal flow dynamics. We conclude that Pkd2 reduction leads to LR defects that cannot be assigned exclusively to its putative role in mediating mechanosensation because indirectly, by modifying cell shape or decreasing cilia length, Pkd2 deficit affects LR flow dynamics.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNJacinto, RaquelSampaio, PedroRoxo-Rosa, MónicaPestana, SaraLopes, Susana S.2021-04-29T23:03:36Z2021-04-012021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116408eng2296-634XPURE: 29415898https://doi.org/10.3389/fcell.2021.624531info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:58:52Zoai:run.unl.pt:10362/116408Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:03.167052Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
title Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
spellingShingle Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
Jacinto, Raquel
cilia length
dand5
flow dynamics
left-right
Pkd2
zebrafish
Developmental Biology
Cell Biology
title_short Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
title_full Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
title_fullStr Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
title_full_unstemmed Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
title_sort Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5
author Jacinto, Raquel
author_facet Jacinto, Raquel
Sampaio, Pedro
Roxo-Rosa, Mónica
Pestana, Sara
Lopes, Susana S.
author_role author
author2 Sampaio, Pedro
Roxo-Rosa, Mónica
Pestana, Sara
Lopes, Susana S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Jacinto, Raquel
Sampaio, Pedro
Roxo-Rosa, Mónica
Pestana, Sara
Lopes, Susana S.
dc.subject.por.fl_str_mv cilia length
dand5
flow dynamics
left-right
Pkd2
zebrafish
Developmental Biology
Cell Biology
topic cilia length
dand5
flow dynamics
left-right
Pkd2
zebrafish
Developmental Biology
Cell Biology
description Funding: This work was supported by the Fundação para a Ciência e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). SL is funded by an FCT CEEC contract for Principal Investigator. The work was also supported by Sociedade Portuguesa de Nefrologia (research grant 2015), iNOVA4Health – UID/Multi/04462/2013 (a program financially supported by Fundação para a Ciência e Tecnologia/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement) and Fundação para a Ciência e Tecnologia (research grant – PTDC/BEXBID/1411/2014). MR-R was supported by national funds through Fundação para a Ciência e Tecnologia, in the context of the celebration of the program contract foreseen in the numbers 4, 5, and 6 of article 23 of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July. Zebrafish used as animal model were reared and maintained in the CEDOC Fish Facility, with the support from the research consortia Congento, co-financed by Lisboa Regional Operational Program (Lisboa2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Fundação para a Ciência e Tecnologia
publishDate 2021
dc.date.none.fl_str_mv 2021-04-29T23:03:36Z
2021-04-01
2021-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116408
url http://hdl.handle.net/10362/116408
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-634X
PURE: 29415898
https://doi.org/10.3389/fcell.2021.624531
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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