Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics

• Autor(a) principal: Santos, Ana Isabel
• Data de Publicação: 2020
• Outros Autores: Lourenco, Ana S., Simão, Sónia, Marques-da-Silva, Dorinda, Santos, Daniela F., Carvalho, Ana Paula Onofre de, Pereira, Ana Catarina, Izquierdo-Álvarez, Alicia, Ramos, Elena, Morato, Esperanza, Marina, Anabel, Martínez-Ruiz, Antonio, Araújo, Inês
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.1/14045
• topic: Nitric oxide; Hippocampus; S-nitrosylation; Seizures; Neural stem cells; Neurogenesis

Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.