Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/106654 https://doi.org/10.1016/j.redox.2020.101457 |
Resumo: | Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC. |
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Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomicsNitric oxide S-nitrosylationNeurogenesisNeural stem cellsNeurogenesis SeizuresHippocampusAnimalsCysteineMiceNitric OxideOxidation-ReductionProtein Processing, Post-TranslationalProteomicsSulfhydryl CompoundsNeural Stem CellsS-NitrosothiolsNitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.Elsevier2020-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106654http://hdl.handle.net/10316/106654https://doi.org/10.1016/j.redox.2020.101457eng22132317Santos, Ana IsabelLourenço, Ana SofiaSimão, SóniaMarques da Silva, DorindaSantos, Daniela FilipaOnofre de Carvalho, Ana PaulaPereira, Ana CatarinaIzquierdo-Álvarez, AliciaRamos, ElenaMorato, EsperanzaMarina, AnabelMartínez-Ruiz, AntonioAraújo, Inês Mariainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-14T08:49:34Zoai:estudogeral.uc.pt:10316/106654Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:23:04.390992Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
title |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
spellingShingle |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics Santos, Ana Isabel Nitric oxide S-nitrosylation Neurogenesis Neural stem cells Neurogenesis Seizures Hippocampus Animals Cysteine Mice Nitric Oxide Oxidation-Reduction Protein Processing, Post-Translational Proteomics Sulfhydryl Compounds Neural Stem Cells S-Nitrosothiols |
title_short |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
title_full |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
title_fullStr |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
title_full_unstemmed |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
title_sort |
Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics |
author |
Santos, Ana Isabel |
author_facet |
Santos, Ana Isabel Lourenço, Ana Sofia Simão, Sónia Marques da Silva, Dorinda Santos, Daniela Filipa Onofre de Carvalho, Ana Paula Pereira, Ana Catarina Izquierdo-Álvarez, Alicia Ramos, Elena Morato, Esperanza Marina, Anabel Martínez-Ruiz, Antonio Araújo, Inês Maria |
author_role |
author |
author2 |
Lourenço, Ana Sofia Simão, Sónia Marques da Silva, Dorinda Santos, Daniela Filipa Onofre de Carvalho, Ana Paula Pereira, Ana Catarina Izquierdo-Álvarez, Alicia Ramos, Elena Morato, Esperanza Marina, Anabel Martínez-Ruiz, Antonio Araújo, Inês Maria |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Ana Isabel Lourenço, Ana Sofia Simão, Sónia Marques da Silva, Dorinda Santos, Daniela Filipa Onofre de Carvalho, Ana Paula Pereira, Ana Catarina Izquierdo-Álvarez, Alicia Ramos, Elena Morato, Esperanza Marina, Anabel Martínez-Ruiz, Antonio Araújo, Inês Maria |
dc.subject.por.fl_str_mv |
Nitric oxide S-nitrosylation Neurogenesis Neural stem cells Neurogenesis Seizures Hippocampus Animals Cysteine Mice Nitric Oxide Oxidation-Reduction Protein Processing, Post-Translational Proteomics Sulfhydryl Compounds Neural Stem Cells S-Nitrosothiols |
topic |
Nitric oxide S-nitrosylation Neurogenesis Neural stem cells Neurogenesis Seizures Hippocampus Animals Cysteine Mice Nitric Oxide Oxidation-Reduction Protein Processing, Post-Translational Proteomics Sulfhydryl Compounds Neural Stem Cells S-Nitrosothiols |
description |
Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/106654 http://hdl.handle.net/10316/106654 https://doi.org/10.1016/j.redox.2020.101457 |
url |
http://hdl.handle.net/10316/106654 https://doi.org/10.1016/j.redox.2020.101457 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
22132317 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134118758318080 |