Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/36499 |
Resumo: | Simultaneous extraction of trypsin inhibitors and soy isoflavones from soybean meal was investigated using the non-destructive phytochemical extraction process, namely aqueous micellar system. The ethoxylated aliphatic alcohols Genapol X-080, Tergitol 15-S-7, and Tergitol 15-S-9, all non-toxic and biodegradable surfactants, were assessed as potential extractants. A Box-Behnken multifactorial design with the application of the Derringer desirability was used to determine the conditions that maximized the trypsin inhibitors and isoflavone extraction while minimizing the protein extraction. The optimum condition of 5% m/m of surfactant in 50 mM aqueous sodium citrate solution pH 4.5, at 45 °C for 45 min, was established for the three surfactants. The novel methodology would allow the extraction of the main soybean antinutritional factors, trypsin inhibitors, and the valuable isoflavones, preserving the nutritional quality of the treated material. This represents a sustainable alternative methodology for industrial purposes due to its low cost, biodegradability, non-toxicity, and easy scaling up. |
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Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimizationAqueous micellar systemsIsoflavone extractionMultifactorial optimizationNon-ionic surfactantTrypsin inhibitorSimultaneous extraction of trypsin inhibitors and soy isoflavones from soybean meal was investigated using the non-destructive phytochemical extraction process, namely aqueous micellar system. The ethoxylated aliphatic alcohols Genapol X-080, Tergitol 15-S-7, and Tergitol 15-S-9, all non-toxic and biodegradable surfactants, were assessed as potential extractants. A Box-Behnken multifactorial design with the application of the Derringer desirability was used to determine the conditions that maximized the trypsin inhibitors and isoflavone extraction while minimizing the protein extraction. The optimum condition of 5% m/m of surfactant in 50 mM aqueous sodium citrate solution pH 4.5, at 45 °C for 45 min, was established for the three surfactants. The novel methodology would allow the extraction of the main soybean antinutritional factors, trypsin inhibitors, and the valuable isoflavones, preserving the nutritional quality of the treated material. This represents a sustainable alternative methodology for industrial purposes due to its low cost, biodegradability, non-toxicity, and easy scaling up.Veritati - Repositório Institucional da Universidade Católica PortuguesaCoscueta, Ezequiel R.Brassesco, María EmiliaMalpiedi, Luciana PellegriniNerli, Bibiana2022-01-19T16:52:43Z2022-01-172022-01-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/36499enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:41:59Zoai:repositorio.ucp.pt:10400.14/36499Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:29:39.969968Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
title |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
spellingShingle |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization Coscueta, Ezequiel R. Aqueous micellar systems Isoflavone extraction Multifactorial optimization Non-ionic surfactant Trypsin inhibitor |
title_short |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
title_full |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
title_fullStr |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
title_full_unstemmed |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
title_sort |
Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization |
author |
Coscueta, Ezequiel R. |
author_facet |
Coscueta, Ezequiel R. Brassesco, María Emilia Malpiedi, Luciana Pellegrini Nerli, Bibiana |
author_role |
author |
author2 |
Brassesco, María Emilia Malpiedi, Luciana Pellegrini Nerli, Bibiana |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Coscueta, Ezequiel R. Brassesco, María Emilia Malpiedi, Luciana Pellegrini Nerli, Bibiana |
dc.subject.por.fl_str_mv |
Aqueous micellar systems Isoflavone extraction Multifactorial optimization Non-ionic surfactant Trypsin inhibitor |
topic |
Aqueous micellar systems Isoflavone extraction Multifactorial optimization Non-ionic surfactant Trypsin inhibitor |
description |
Simultaneous extraction of trypsin inhibitors and soy isoflavones from soybean meal was investigated using the non-destructive phytochemical extraction process, namely aqueous micellar system. The ethoxylated aliphatic alcohols Genapol X-080, Tergitol 15-S-7, and Tergitol 15-S-9, all non-toxic and biodegradable surfactants, were assessed as potential extractants. A Box-Behnken multifactorial design with the application of the Derringer desirability was used to determine the conditions that maximized the trypsin inhibitors and isoflavone extraction while minimizing the protein extraction. The optimum condition of 5% m/m of surfactant in 50 mM aqueous sodium citrate solution pH 4.5, at 45 °C for 45 min, was established for the three surfactants. The novel methodology would allow the extraction of the main soybean antinutritional factors, trypsin inhibitors, and the valuable isoflavones, preserving the nutritional quality of the treated material. This represents a sustainable alternative methodology for industrial purposes due to its low cost, biodegradability, non-toxicity, and easy scaling up. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-19T16:52:43Z 2022-01-17 2022-01-17T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/36499 |
url |
http://hdl.handle.net/10400.14/36499 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132017728684032 |