Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization

Detalhes bibliográficos
Autor(a) principal: Coscueta, Ezequiel R.
Data de Publicação: 2022
Outros Autores: Malpiedi, Luciana Pellegrini, Pintado, Maria Manuela, Nerli, Bibiana B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/39426
Resumo: This work aimed to develop a novel methodology based on aqueous micellar systems (AMS), for producing soy protein concentrates (SPC) from soybean flour and recovering high-valuable bioactive compounds as by-products. Ethoxylated aliphatic alcohols Tergitol 15-S-7 and Tergitol 15-S-9, non-toxic and biodegradable surfactants, were selected to form the AMS. The methodology consisted of an extractive stage of soybean flour with AMS, which rendered both a pellet, i.e., the SPC, and a supernatant containing the extracted bioactive compounds. The latter was further heated above the cloud point temperature, thus resulting in a biphasic system formed by a micelle-rich phase (MP) and an aqueous phase (AP). Obtained SPC showed a noticeable loss (∼90%) of trypsin inhibitor activity, a total protein content close to 60%, soluble protein amounts varying from 19% to 34%, and remarkable released (by simulated digestion) antioxidant and antihypertensive activities. Those indicators are similar to or even better than those corresponding to SPC from the classical acid-extraction method. The AMS also exhibited an enhanced efficiency for extracting antinutrients such as non-digestible oligosaccharides, trypsin inhibitors, and lectins mostly recovered at the AP and separated from isoflavones, which were concentrated and isolated at the MP. The recovery of all the mentioned bioactive compounds, whether beneficial or undesirable, broadens their uses in research, food, and pharmacological fields. This successful performance, simplicity, scalability, and sustainability make the proposed AMS-based extraction a powerful tool for processing plant derivatives and valorizing their by-products.
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spelling Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorizationAqueous micellar two-phase systemIsoflavonesTrypsin inhibitorsGalactooligosaccharidesAntioxidant activityAntihypertensive activityThis work aimed to develop a novel methodology based on aqueous micellar systems (AMS), for producing soy protein concentrates (SPC) from soybean flour and recovering high-valuable bioactive compounds as by-products. Ethoxylated aliphatic alcohols Tergitol 15-S-7 and Tergitol 15-S-9, non-toxic and biodegradable surfactants, were selected to form the AMS. The methodology consisted of an extractive stage of soybean flour with AMS, which rendered both a pellet, i.e., the SPC, and a supernatant containing the extracted bioactive compounds. The latter was further heated above the cloud point temperature, thus resulting in a biphasic system formed by a micelle-rich phase (MP) and an aqueous phase (AP). Obtained SPC showed a noticeable loss (∼90%) of trypsin inhibitor activity, a total protein content close to 60%, soluble protein amounts varying from 19% to 34%, and remarkable released (by simulated digestion) antioxidant and antihypertensive activities. Those indicators are similar to or even better than those corresponding to SPC from the classical acid-extraction method. The AMS also exhibited an enhanced efficiency for extracting antinutrients such as non-digestible oligosaccharides, trypsin inhibitors, and lectins mostly recovered at the AP and separated from isoflavones, which were concentrated and isolated at the MP. The recovery of all the mentioned bioactive compounds, whether beneficial or undesirable, broadens their uses in research, food, and pharmacological fields. This successful performance, simplicity, scalability, and sustainability make the proposed AMS-based extraction a powerful tool for processing plant derivatives and valorizing their by-products.Veritati - Repositório Institucional da Universidade Católica PortuguesaCoscueta, Ezequiel R.Malpiedi, Luciana PellegriniPintado, Maria ManuelaNerli, Bibiana B.2022-11-29T12:27:29Z2023-04-012023-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/39426eng0268-005X10.1016/j.foodhyd.2022.108314000917359400004info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:44:55Zoai:repositorio.ucp.pt:10400.14/39426Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:32:16.226393Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
title Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
spellingShingle Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
Coscueta, Ezequiel R.
Aqueous micellar two-phase system
Isoflavones
Trypsin inhibitors
Galactooligosaccharides
Antioxidant activity
Antihypertensive activity
title_short Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
title_full Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
title_fullStr Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
title_full_unstemmed Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
title_sort Production of soy protein concentrate with the recovery of bioactive compounds: from destruction to valorization
author Coscueta, Ezequiel R.
author_facet Coscueta, Ezequiel R.
Malpiedi, Luciana Pellegrini
Pintado, Maria Manuela
Nerli, Bibiana B.
author_role author
author2 Malpiedi, Luciana Pellegrini
Pintado, Maria Manuela
Nerli, Bibiana B.
author2_role author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Coscueta, Ezequiel R.
Malpiedi, Luciana Pellegrini
Pintado, Maria Manuela
Nerli, Bibiana B.
dc.subject.por.fl_str_mv Aqueous micellar two-phase system
Isoflavones
Trypsin inhibitors
Galactooligosaccharides
Antioxidant activity
Antihypertensive activity
topic Aqueous micellar two-phase system
Isoflavones
Trypsin inhibitors
Galactooligosaccharides
Antioxidant activity
Antihypertensive activity
description This work aimed to develop a novel methodology based on aqueous micellar systems (AMS), for producing soy protein concentrates (SPC) from soybean flour and recovering high-valuable bioactive compounds as by-products. Ethoxylated aliphatic alcohols Tergitol 15-S-7 and Tergitol 15-S-9, non-toxic and biodegradable surfactants, were selected to form the AMS. The methodology consisted of an extractive stage of soybean flour with AMS, which rendered both a pellet, i.e., the SPC, and a supernatant containing the extracted bioactive compounds. The latter was further heated above the cloud point temperature, thus resulting in a biphasic system formed by a micelle-rich phase (MP) and an aqueous phase (AP). Obtained SPC showed a noticeable loss (∼90%) of trypsin inhibitor activity, a total protein content close to 60%, soluble protein amounts varying from 19% to 34%, and remarkable released (by simulated digestion) antioxidant and antihypertensive activities. Those indicators are similar to or even better than those corresponding to SPC from the classical acid-extraction method. The AMS also exhibited an enhanced efficiency for extracting antinutrients such as non-digestible oligosaccharides, trypsin inhibitors, and lectins mostly recovered at the AP and separated from isoflavones, which were concentrated and isolated at the MP. The recovery of all the mentioned bioactive compounds, whether beneficial or undesirable, broadens their uses in research, food, and pharmacological fields. This successful performance, simplicity, scalability, and sustainability make the proposed AMS-based extraction a powerful tool for processing plant derivatives and valorizing their by-products.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-29T12:27:29Z
2023-04-01
2023-04-01T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/39426
url http://hdl.handle.net/10400.14/39426
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0268-005X
10.1016/j.foodhyd.2022.108314
000917359400004
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eu_rights_str_mv openAccess
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