Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/148011 |
Resumo: | Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors. |
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Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.Staphylococcus aureus/geneticsStaphylococcus/geneticsMultidrug Resistance-Associated Proteins/geneticsAnti-Bacterial Agents/pharmacologyPhylogenyBacterial Proteins/geneticsMicrobial Sensitivity TestsQR MicrobiologyRA0421 Public health. Hygiene. Preventive MedicineT TechnologyMedical Laboratory TechnologyPharmacology, Toxicology and Pharmaceutics(all)MicrobiologyMolecular BiologySDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureFunding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors.NorA is one of the main native MDR efflux pumps of Staphylococcus aureus, contributing to reduced susceptibility towards fluoroquinolones and biocides, but little is known about its variability within S. aureus or its distribution and conservation among other staphylococci. We screened for sequences homologous to S. aureus norA and found it in 61 out of the 63 Staphylococcus species described. To the best of our knowledge, this is the first study to report the occurrence of norA across the Staphylococcus genus. The norA phylogenetic tree follows the evolutionary relations of staphylococci and the closely related Mammalliicoccus genus. Comparative analyses suggest a conservation of the NorA function in staphylococci. We also analyzed the variability of norA within S. aureus, for which there are several circulating norA alleles, differing up to 10% at the nucleotide level, which may hamper proper norA detection. We demonstrate the applicability of a PCR-based algorithm to detect and differentiate norA alleles in 52 S. aureus representing a wider collection of 89 isolates from different hosts. Our results highlight the prevalence of norAI and norAII in different settings and the association of norA alleles with specific S. aureus clonal lineages. Ultimately, it confirms the applicability of our PCR-based algorithm to rapidly detect and assign the different norA alleles, a trait that may impact antimicrobial efflux capacity and the search for potential NorA inhibitors.TB, HIV and opportunistic diseases and pathogens (THOP)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNFerreira, CarolinaAbrantes, PatríciaCosta, Sofia SantosViveiros, MiguelCouto, Isabel2023-01-23T22:15:23Z2022-12-042022-12-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/148011eng1422-0067PURE: 48313477https://doi.org/10.3390/ijms232315306info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:08:23Zoai:run.unl.pt:10362/148011Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:08:23Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
title |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
spellingShingle |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. Ferreira, Carolina Staphylococcus aureus/genetics Staphylococcus/genetics Multidrug Resistance-Associated Proteins/genetics Anti-Bacterial Agents/pharmacology Phylogeny Bacterial Proteins/genetics Microbial Sensitivity Tests QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine T Technology Medical Laboratory Technology Pharmacology, Toxicology and Pharmaceutics(all) Microbiology Molecular Biology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
title_short |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
title_full |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
title_fullStr |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
title_full_unstemmed |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
title_sort |
Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. |
author |
Ferreira, Carolina |
author_facet |
Ferreira, Carolina Abrantes, Patrícia Costa, Sofia Santos Viveiros, Miguel Couto, Isabel |
author_role |
author |
author2 |
Abrantes, Patrícia Costa, Sofia Santos Viveiros, Miguel Couto, Isabel |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
TB, HIV and opportunistic diseases and pathogens (THOP) Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
dc.contributor.author.fl_str_mv |
Ferreira, Carolina Abrantes, Patrícia Costa, Sofia Santos Viveiros, Miguel Couto, Isabel |
dc.subject.por.fl_str_mv |
Staphylococcus aureus/genetics Staphylococcus/genetics Multidrug Resistance-Associated Proteins/genetics Anti-Bacterial Agents/pharmacology Phylogeny Bacterial Proteins/genetics Microbial Sensitivity Tests QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine T Technology Medical Laboratory Technology Pharmacology, Toxicology and Pharmaceutics(all) Microbiology Molecular Biology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
topic |
Staphylococcus aureus/genetics Staphylococcus/genetics Multidrug Resistance-Associated Proteins/genetics Anti-Bacterial Agents/pharmacology Phylogeny Bacterial Proteins/genetics Microbial Sensitivity Tests QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine T Technology Medical Laboratory Technology Pharmacology, Toxicology and Pharmaceutics(all) Microbiology Molecular Biology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
description |
Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-04 2022-12-04T00:00:00Z 2023-01-23T22:15:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/148011 |
url |
http://hdl.handle.net/10362/148011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1422-0067 PURE: 48313477 https://doi.org/10.3390/ijms232315306 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545910433873920 |