Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.

Detalhes bibliográficos
Autor(a) principal: Ferreira, Carolina
Data de Publicação: 2022
Outros Autores: Abrantes, Patrícia, Costa, Sofia Santos, Viveiros, Miguel, Couto, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/148011
Resumo: Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors.
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spelling Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.Staphylococcus aureus/geneticsStaphylococcus/geneticsMultidrug Resistance-Associated Proteins/geneticsAnti-Bacterial Agents/pharmacologyPhylogenyBacterial Proteins/geneticsMicrobial Sensitivity TestsQR MicrobiologyRA0421 Public health. Hygiene. Preventive MedicineT TechnologyMedical Laboratory TechnologyPharmacology, Toxicology and Pharmaceutics(all)MicrobiologyMolecular BiologySDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureFunding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors.NorA is one of the main native MDR efflux pumps of Staphylococcus aureus, contributing to reduced susceptibility towards fluoroquinolones and biocides, but little is known about its variability within S. aureus or its distribution and conservation among other staphylococci. We screened for sequences homologous to S. aureus norA and found it in 61 out of the 63 Staphylococcus species described. To the best of our knowledge, this is the first study to report the occurrence of norA across the Staphylococcus genus. The norA phylogenetic tree follows the evolutionary relations of staphylococci and the closely related Mammalliicoccus genus. Comparative analyses suggest a conservation of the NorA function in staphylococci. We also analyzed the variability of norA within S. aureus, for which there are several circulating norA alleles, differing up to 10% at the nucleotide level, which may hamper proper norA detection. We demonstrate the applicability of a PCR-based algorithm to detect and differentiate norA alleles in 52 S. aureus representing a wider collection of 89 isolates from different hosts. Our results highlight the prevalence of norAI and norAII in different settings and the association of norA alleles with specific S. aureus clonal lineages. Ultimately, it confirms the applicability of our PCR-based algorithm to rapidly detect and assign the different norA alleles, a trait that may impact antimicrobial efflux capacity and the search for potential NorA inhibitors.TB, HIV and opportunistic diseases and pathogens (THOP)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNFerreira, CarolinaAbrantes, PatríciaCosta, Sofia SantosViveiros, MiguelCouto, Isabel2023-01-23T22:15:23Z2022-12-042022-12-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/148011eng1422-0067PURE: 48313477https://doi.org/10.3390/ijms232315306info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:29:15Zoai:run.unl.pt:10362/148011Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:08.748308Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
title Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
spellingShingle Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
Ferreira, Carolina
Staphylococcus aureus/genetics
Staphylococcus/genetics
Multidrug Resistance-Associated Proteins/genetics
Anti-Bacterial Agents/pharmacology
Phylogeny
Bacterial Proteins/genetics
Microbial Sensitivity Tests
QR Microbiology
RA0421 Public health. Hygiene. Preventive Medicine
T Technology
Medical Laboratory Technology
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
title_short Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
title_full Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
title_fullStr Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
title_full_unstemmed Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
title_sort Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus.
author Ferreira, Carolina
author_facet Ferreira, Carolina
Abrantes, Patrícia
Costa, Sofia Santos
Viveiros, Miguel
Couto, Isabel
author_role author
author2 Abrantes, Patrícia
Costa, Sofia Santos
Viveiros, Miguel
Couto, Isabel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv TB, HIV and opportunistic diseases and pathogens (THOP)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Ferreira, Carolina
Abrantes, Patrícia
Costa, Sofia Santos
Viveiros, Miguel
Couto, Isabel
dc.subject.por.fl_str_mv Staphylococcus aureus/genetics
Staphylococcus/genetics
Multidrug Resistance-Associated Proteins/genetics
Anti-Bacterial Agents/pharmacology
Phylogeny
Bacterial Proteins/genetics
Microbial Sensitivity Tests
QR Microbiology
RA0421 Public health. Hygiene. Preventive Medicine
T Technology
Medical Laboratory Technology
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
topic Staphylococcus aureus/genetics
Staphylococcus/genetics
Multidrug Resistance-Associated Proteins/genetics
Anti-Bacterial Agents/pharmacology
Phylogeny
Bacterial Proteins/genetics
Microbial Sensitivity Tests
QR Microbiology
RA0421 Public health. Hygiene. Preventive Medicine
T Technology
Medical Laboratory Technology
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
description Funding Information: This work was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grant 2021.05063.BD (C.F) and funds to GHTM (UID/04413/2020). Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-04
2022-12-04T00:00:00Z
2023-01-23T22:15:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/148011
url http://hdl.handle.net/10362/148011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1422-0067
PURE: 48313477
https://doi.org/10.3390/ijms232315306
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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