Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity

Detalhes bibliográficos
Autor(a) principal: Silva, Osmar N.
Data de Publicação: 2016
Outros Autores: Alves, Eliane S. F., Fuente-Núñez, César de la, Ribeiro, Suzana M., Mandal, Santi M., Gaspar, Diana, Veiga, Ana S., Castanho, Miguel A. R. B., Andrade, Cesar A. S., Nascimento, Jessica M., Fensterseifer, Isabel C. M., Porto, William F., Correa, Jose R., Hancock, Robert. E. W., Korpole, Suresh Korpole, Oliveira, Aline L., Liao, Luciano M., Franco, Octavio L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/32817
Resumo: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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spelling Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activityThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/Clavanins is a class of peptides (23aa) histidine-rich, free of post-translational modifications. Clavanins have been studied largely for their ability to disrupt bacterial membranes. In the present study, the interaction of clavanin A with membranes was assessed by dynamic light scattering, zeta potential and permeabilization assays. We observed through those assays that clavanin A lysis bacterial cells at concentrations corresponding to its MIC. Further, the structure and function of clavanin A was investigated. To better understand how clavanin interacted with bacteria, its NMR structure was elucidated. The solution state NMR structure of clavanin A in the presence of TFE-d3 indicated an α-helical conformation. Secondary structures, based on circular dichroism measurements in anionic sodium dodecyl sulfate (SDS) and TFE (2,2,2-trifluorethanol), in silico lipid-peptide docking and molecular simulations with lipids DPPC and DOPC revealed that clavanin A can adopt a variety of folds, possibly influencing its different functions. Microcalorimetry assays revealed that clavanin A was capable of discriminating between different lipids. Finally, clavanin A was found to eradicate bacterial biofilms representing a previously unrecognized function.We would like to thank CNPq, CAPES (Ciências sem Fronteiras), FAPDF and FUNDECT. D.G. acknowledges Fundação para a Ciência e a Tecnologia - Ministério da Educação e Ciência (FCT-MEC, Portugal) for fellowship SFRH/BPD/73500/2010 and A.S.V. for funding within the FCT Investigator Programme (IF/00803/2012).Portland PressRepositório da Universidade de LisboaSilva, Osmar N.Alves, Eliane S. F.Fuente-Núñez, César de laRibeiro, Suzana M.Mandal, Santi M.Gaspar, DianaVeiga, Ana S.Castanho, Miguel A. R. B.Andrade, Cesar A. S.Nascimento, Jessica M.Fensterseifer, Isabel C. M.Porto, William F.Correa, Jose R.Hancock, Robert. E. W.Korpole, Suresh KorpoleOliveira, Aline L.Liao, Luciano M.Franco, Octavio L.2018-04-16T13:35:59Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/32817eng0144-846310.1038/srep27128info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:27:04Zoai:repositorio.ul.pt:10451/32817Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:47:54.324067Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
title Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
spellingShingle Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
Silva, Osmar N.
title_short Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
title_full Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
title_fullStr Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
title_full_unstemmed Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
title_sort Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
author Silva, Osmar N.
author_facet Silva, Osmar N.
Alves, Eliane S. F.
Fuente-Núñez, César de la
Ribeiro, Suzana M.
Mandal, Santi M.
Gaspar, Diana
Veiga, Ana S.
Castanho, Miguel A. R. B.
Andrade, Cesar A. S.
Nascimento, Jessica M.
Fensterseifer, Isabel C. M.
Porto, William F.
Correa, Jose R.
Hancock, Robert. E. W.
Korpole, Suresh Korpole
Oliveira, Aline L.
Liao, Luciano M.
Franco, Octavio L.
author_role author
author2 Alves, Eliane S. F.
Fuente-Núñez, César de la
Ribeiro, Suzana M.
Mandal, Santi M.
Gaspar, Diana
Veiga, Ana S.
Castanho, Miguel A. R. B.
Andrade, Cesar A. S.
Nascimento, Jessica M.
Fensterseifer, Isabel C. M.
Porto, William F.
Correa, Jose R.
Hancock, Robert. E. W.
Korpole, Suresh Korpole
Oliveira, Aline L.
Liao, Luciano M.
Franco, Octavio L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva, Osmar N.
Alves, Eliane S. F.
Fuente-Núñez, César de la
Ribeiro, Suzana M.
Mandal, Santi M.
Gaspar, Diana
Veiga, Ana S.
Castanho, Miguel A. R. B.
Andrade, Cesar A. S.
Nascimento, Jessica M.
Fensterseifer, Isabel C. M.
Porto, William F.
Correa, Jose R.
Hancock, Robert. E. W.
Korpole, Suresh Korpole
Oliveira, Aline L.
Liao, Luciano M.
Franco, Octavio L.
description This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2018-04-16T13:35:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/32817
url http://hdl.handle.net/10451/32817
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0144-8463
10.1038/srep27128
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dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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