Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/32817 |
Resumo: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
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oai:repositorio.ul.pt:10451/32817 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activityThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/Clavanins is a class of peptides (23aa) histidine-rich, free of post-translational modifications. Clavanins have been studied largely for their ability to disrupt bacterial membranes. In the present study, the interaction of clavanin A with membranes was assessed by dynamic light scattering, zeta potential and permeabilization assays. We observed through those assays that clavanin A lysis bacterial cells at concentrations corresponding to its MIC. Further, the structure and function of clavanin A was investigated. To better understand how clavanin interacted with bacteria, its NMR structure was elucidated. The solution state NMR structure of clavanin A in the presence of TFE-d3 indicated an α-helical conformation. Secondary structures, based on circular dichroism measurements in anionic sodium dodecyl sulfate (SDS) and TFE (2,2,2-trifluorethanol), in silico lipid-peptide docking and molecular simulations with lipids DPPC and DOPC revealed that clavanin A can adopt a variety of folds, possibly influencing its different functions. Microcalorimetry assays revealed that clavanin A was capable of discriminating between different lipids. Finally, clavanin A was found to eradicate bacterial biofilms representing a previously unrecognized function.We would like to thank CNPq, CAPES (Ciências sem Fronteiras), FAPDF and FUNDECT. D.G. acknowledges Fundação para a Ciência e a Tecnologia - Ministério da Educação e Ciência (FCT-MEC, Portugal) for fellowship SFRH/BPD/73500/2010 and A.S.V. for funding within the FCT Investigator Programme (IF/00803/2012).Portland PressRepositório da Universidade de LisboaSilva, Osmar N.Alves, Eliane S. F.Fuente-Núñez, César de laRibeiro, Suzana M.Mandal, Santi M.Gaspar, DianaVeiga, Ana S.Castanho, Miguel A. R. B.Andrade, Cesar A. S.Nascimento, Jessica M.Fensterseifer, Isabel C. M.Porto, William F.Correa, Jose R.Hancock, Robert. E. W.Korpole, Suresh KorpoleOliveira, Aline L.Liao, Luciano M.Franco, Octavio L.2018-04-16T13:35:59Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/32817eng0144-846310.1038/srep27128info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:27:04Zoai:repositorio.ul.pt:10451/32817Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:47:54.324067Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
title |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
spellingShingle |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity Silva, Osmar N. |
title_short |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
title_full |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
title_fullStr |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
title_full_unstemmed |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
title_sort |
Structural studies of a lipid-binding peptide from tunicate hemocytes with anti-biofilm activity |
author |
Silva, Osmar N. |
author_facet |
Silva, Osmar N. Alves, Eliane S. F. Fuente-Núñez, César de la Ribeiro, Suzana M. Mandal, Santi M. Gaspar, Diana Veiga, Ana S. Castanho, Miguel A. R. B. Andrade, Cesar A. S. Nascimento, Jessica M. Fensterseifer, Isabel C. M. Porto, William F. Correa, Jose R. Hancock, Robert. E. W. Korpole, Suresh Korpole Oliveira, Aline L. Liao, Luciano M. Franco, Octavio L. |
author_role |
author |
author2 |
Alves, Eliane S. F. Fuente-Núñez, César de la Ribeiro, Suzana M. Mandal, Santi M. Gaspar, Diana Veiga, Ana S. Castanho, Miguel A. R. B. Andrade, Cesar A. S. Nascimento, Jessica M. Fensterseifer, Isabel C. M. Porto, William F. Correa, Jose R. Hancock, Robert. E. W. Korpole, Suresh Korpole Oliveira, Aline L. Liao, Luciano M. Franco, Octavio L. |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Silva, Osmar N. Alves, Eliane S. F. Fuente-Núñez, César de la Ribeiro, Suzana M. Mandal, Santi M. Gaspar, Diana Veiga, Ana S. Castanho, Miguel A. R. B. Andrade, Cesar A. S. Nascimento, Jessica M. Fensterseifer, Isabel C. M. Porto, William F. Correa, Jose R. Hancock, Robert. E. W. Korpole, Suresh Korpole Oliveira, Aline L. Liao, Luciano M. Franco, Octavio L. |
description |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2018-04-16T13:35:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/32817 |
url |
http://hdl.handle.net/10451/32817 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0144-8463 10.1038/srep27128 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Portland Press |
publisher.none.fl_str_mv |
Portland Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134406833602560 |