The ecdysteroidome of Drosophila: influence of diet and development
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/520 |
Resumo: | Ecdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol. |
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The ecdysteroidome of Drosophila: influence of diet and developmentDrosophila melanogasterLC-MS/MSDietary sterolsEcdysoneEcdysteroidsMakisterone AEcdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol.Max Planck Geselleschaft, Deutsche Forschungsgemeinschaft (TRR 83, projects A17 and A19), European Molecular Biology Organization Long Term Fellowship, University Pierre and Marie Curie.Company of BiologistsARCALavrynenko, O.Rodenfels, J.Carvalho, M.Dye, N. A.Lafont, R.Eaton, S.Shevchenko, A.2015-11-23T15:58:40Z2015-11-012015-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/520engThe ecdysteroidome of Drosophila: influence of diet and development Oksana Lavrynenko, Jonathan Rodenfels, Maria Carvalho, Natalie A. Dye, Rene Lafont, Suzanne Eaton, Andrej Shevchenko Development 2015 142: 3758-3768; doi: 10.1242/dev.12498210.1242/dev.124982info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:53Zoai:arca.igc.gulbenkian.pt:10400.7/520Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:46.674324Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The ecdysteroidome of Drosophila: influence of diet and development |
title |
The ecdysteroidome of Drosophila: influence of diet and development |
spellingShingle |
The ecdysteroidome of Drosophila: influence of diet and development Lavrynenko, O. Drosophila melanogaster LC-MS/MS Dietary sterols Ecdysone Ecdysteroids Makisterone A |
title_short |
The ecdysteroidome of Drosophila: influence of diet and development |
title_full |
The ecdysteroidome of Drosophila: influence of diet and development |
title_fullStr |
The ecdysteroidome of Drosophila: influence of diet and development |
title_full_unstemmed |
The ecdysteroidome of Drosophila: influence of diet and development |
title_sort |
The ecdysteroidome of Drosophila: influence of diet and development |
author |
Lavrynenko, O. |
author_facet |
Lavrynenko, O. Rodenfels, J. Carvalho, M. Dye, N. A. Lafont, R. Eaton, S. Shevchenko, A. |
author_role |
author |
author2 |
Rodenfels, J. Carvalho, M. Dye, N. A. Lafont, R. Eaton, S. Shevchenko, A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Lavrynenko, O. Rodenfels, J. Carvalho, M. Dye, N. A. Lafont, R. Eaton, S. Shevchenko, A. |
dc.subject.por.fl_str_mv |
Drosophila melanogaster LC-MS/MS Dietary sterols Ecdysone Ecdysteroids Makisterone A |
topic |
Drosophila melanogaster LC-MS/MS Dietary sterols Ecdysone Ecdysteroids Makisterone A |
description |
Ecdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11-23T15:58:40Z 2015-11-01 2015-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/520 |
url |
http://hdl.handle.net/10400.7/520 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
The ecdysteroidome of Drosophila: influence of diet and development Oksana Lavrynenko, Jonathan Rodenfels, Maria Carvalho, Natalie A. Dye, Rene Lafont, Suzanne Eaton, Andrej Shevchenko Development 2015 142: 3758-3768; doi: 10.1242/dev.124982 10.1242/dev.124982 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Company of Biologists |
publisher.none.fl_str_mv |
Company of Biologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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