BC nanofibres: in vitro study of genotoxicity and cell proliferation
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/9608 |
Resumo: | Nanomaterials have unusual properties not found in the bulk materials, which can be exploited in numerous applications such as biosensing, electronics, scaffolds for tissue engineering, diagnostics and drug delivery. However, research in the past few years has turned up a range of potential health hazards, which has given birth to the new discipline of nanotoxicology. Bacterial cellulose (BC) is a promising material for biomedical applications, namely due its biocompatibility. Although BC has been shown not to be cytotoxic or genotoxic, the properties of isolated BC nanofibres (NFs) on cells and tissues has never been analysed. Considering the toxicity associated to other fibre-shaped nanoparticles, it seems crucial to evaluate the toxicity associated to the BC-NFs. In thiswork, nanofibreswere produced frombacterial cellulose by a combination of acid and ultrasonic treatment. The genotoxicity of nanofibres from bacterial cellulosewas analysed in vitro, using techniques previously demonstrated to detect the genotoxicity of fibrous nanoparticles. The results from single cell gel electrophoresis (also known as comet assay) and the Salmonella reversion assays showed that NFs are not genotoxicity under the conditions tested. A proliferation assay using fibroblasts and CHO cells reveals a slight reduction in the proliferation rate, although no modification in the cell morphology is observed. |
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BC nanofibres: in vitro study of genotoxicity and cell proliferationNanofibresNonotoxicologyBacterial celluloseGenotoxicityScience & TechnologyNanomaterials have unusual properties not found in the bulk materials, which can be exploited in numerous applications such as biosensing, electronics, scaffolds for tissue engineering, diagnostics and drug delivery. However, research in the past few years has turned up a range of potential health hazards, which has given birth to the new discipline of nanotoxicology. Bacterial cellulose (BC) is a promising material for biomedical applications, namely due its biocompatibility. Although BC has been shown not to be cytotoxic or genotoxic, the properties of isolated BC nanofibres (NFs) on cells and tissues has never been analysed. Considering the toxicity associated to other fibre-shaped nanoparticles, it seems crucial to evaluate the toxicity associated to the BC-NFs. In thiswork, nanofibreswere produced frombacterial cellulose by a combination of acid and ultrasonic treatment. The genotoxicity of nanofibres from bacterial cellulosewas analysed in vitro, using techniques previously demonstrated to detect the genotoxicity of fibrous nanoparticles. The results from single cell gel electrophoresis (also known as comet assay) and the Salmonella reversion assays showed that NFs are not genotoxicity under the conditions tested. A proliferation assay using fibroblasts and CHO cells reveals a slight reduction in the proliferation rate, although no modification in the cell morphology is observed.Fundação para a Ciência e a Tecnologia (FCT)Conselho Nacional de Desenvolvimento Científico e Tecnológico Brazil (CNPq)CAPES Brazil (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)ElsevierUniversidade do MinhoMoreira, Susana Margarida GomesSilva, Naisandra BezerraAlmeida-Lima, JailmaRocha, Hugo Alexandre OliveiraMedeiros, Silvia Regina BatistuzzoAlves Junior, ClodomiroGama, F. M.2009-092009-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9608eng"Toxicology Letters". ISSN 0378-4274. 189:3 (Sept. 2008) 235-241.0378-427410.1016/j.toxlet.2009.06.84919524649info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:38Zoai:repositorium.sdum.uminho.pt:1822/9608Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:29.902928Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
title |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
spellingShingle |
BC nanofibres: in vitro study of genotoxicity and cell proliferation Moreira, Susana Margarida Gomes Nanofibres Nonotoxicology Bacterial cellulose Genotoxicity Science & Technology |
title_short |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
title_full |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
title_fullStr |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
title_full_unstemmed |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
title_sort |
BC nanofibres: in vitro study of genotoxicity and cell proliferation |
author |
Moreira, Susana Margarida Gomes |
author_facet |
Moreira, Susana Margarida Gomes Silva, Naisandra Bezerra Almeida-Lima, Jailma Rocha, Hugo Alexandre Oliveira Medeiros, Silvia Regina Batistuzzo Alves Junior, Clodomiro Gama, F. M. |
author_role |
author |
author2 |
Silva, Naisandra Bezerra Almeida-Lima, Jailma Rocha, Hugo Alexandre Oliveira Medeiros, Silvia Regina Batistuzzo Alves Junior, Clodomiro Gama, F. M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Moreira, Susana Margarida Gomes Silva, Naisandra Bezerra Almeida-Lima, Jailma Rocha, Hugo Alexandre Oliveira Medeiros, Silvia Regina Batistuzzo Alves Junior, Clodomiro Gama, F. M. |
dc.subject.por.fl_str_mv |
Nanofibres Nonotoxicology Bacterial cellulose Genotoxicity Science & Technology |
topic |
Nanofibres Nonotoxicology Bacterial cellulose Genotoxicity Science & Technology |
description |
Nanomaterials have unusual properties not found in the bulk materials, which can be exploited in numerous applications such as biosensing, electronics, scaffolds for tissue engineering, diagnostics and drug delivery. However, research in the past few years has turned up a range of potential health hazards, which has given birth to the new discipline of nanotoxicology. Bacterial cellulose (BC) is a promising material for biomedical applications, namely due its biocompatibility. Although BC has been shown not to be cytotoxic or genotoxic, the properties of isolated BC nanofibres (NFs) on cells and tissues has never been analysed. Considering the toxicity associated to other fibre-shaped nanoparticles, it seems crucial to evaluate the toxicity associated to the BC-NFs. In thiswork, nanofibreswere produced frombacterial cellulose by a combination of acid and ultrasonic treatment. The genotoxicity of nanofibres from bacterial cellulosewas analysed in vitro, using techniques previously demonstrated to detect the genotoxicity of fibrous nanoparticles. The results from single cell gel electrophoresis (also known as comet assay) and the Salmonella reversion assays showed that NFs are not genotoxicity under the conditions tested. A proliferation assay using fibroblasts and CHO cells reveals a slight reduction in the proliferation rate, although no modification in the cell morphology is observed. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09 2009-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/9608 |
url |
http://hdl.handle.net/1822/9608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Toxicology Letters". ISSN 0378-4274. 189:3 (Sept. 2008) 235-241. 0378-4274 10.1016/j.toxlet.2009.06.849 19524649 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132274022678528 |