Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/3066 |
Resumo: | ABSTRACT: Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct production of bioethanol from microalgae through an exclusive autotrophic route. It can be concluded that the recombinant Synechocystis UL 030 containing two copies of the genes per genome was the most tolerant to ethanol. Nevertheless, to implement a production process using recombinant strains, the bioethanol produced will be required to be continuously extracted from the culture media via a membrane-based technological process for example to prevent detrimental effects on the biomass. The results presented here are of significance in defining the maximum threshold for bulk ethanol concentration in production media. |
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Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometryEthanolSynechocystisMembrane permeabilityEnzymatic activityFlow cytometryABSTRACT: Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct production of bioethanol from microalgae through an exclusive autotrophic route. It can be concluded that the recombinant Synechocystis UL 030 containing two copies of the genes per genome was the most tolerant to ethanol. Nevertheless, to implement a production process using recombinant strains, the bioethanol produced will be required to be continuously extracted from the culture media via a membrane-based technological process for example to prevent detrimental effects on the biomass. The results presented here are of significance in defining the maximum threshold for bulk ethanol concentration in production media.ElsevierRepositório do LNEGSilva, Teresa Lopes daPassarinho, PaulaGalriça, RicardoZenóglio, AfonsoArmshaw, PatríciaPembroke, J. TonySheahan, ConReis, AlbertoGírio, Francisco2018-09-24T11:15:33Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3066engSilva, T. Lopes da; Passarinho, Paula C.; Galriça, Ricardo...[et.al.]. - Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry. In: Biotechnology Reports, Vol. 17, p. 137-1472215-017Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-23T06:27:19Zoai:repositorio.lneg.pt:10400.9/3066Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:36:13.557272Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
title |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
spellingShingle |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry Silva, Teresa Lopes da Ethanol Synechocystis Membrane permeability Enzymatic activity Flow cytometry |
title_short |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
title_full |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
title_fullStr |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
title_full_unstemmed |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
title_sort |
Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry |
author |
Silva, Teresa Lopes da |
author_facet |
Silva, Teresa Lopes da Passarinho, Paula Galriça, Ricardo Zenóglio, Afonso Armshaw, Patrícia Pembroke, J. Tony Sheahan, Con Reis, Alberto Gírio, Francisco |
author_role |
author |
author2 |
Passarinho, Paula Galriça, Ricardo Zenóglio, Afonso Armshaw, Patrícia Pembroke, J. Tony Sheahan, Con Reis, Alberto Gírio, Francisco |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Silva, Teresa Lopes da Passarinho, Paula Galriça, Ricardo Zenóglio, Afonso Armshaw, Patrícia Pembroke, J. Tony Sheahan, Con Reis, Alberto Gírio, Francisco |
dc.subject.por.fl_str_mv |
Ethanol Synechocystis Membrane permeability Enzymatic activity Flow cytometry |
topic |
Ethanol Synechocystis Membrane permeability Enzymatic activity Flow cytometry |
description |
ABSTRACT: Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct production of bioethanol from microalgae through an exclusive autotrophic route. It can be concluded that the recombinant Synechocystis UL 030 containing two copies of the genes per genome was the most tolerant to ethanol. Nevertheless, to implement a production process using recombinant strains, the bioethanol produced will be required to be continuously extracted from the culture media via a membrane-based technological process for example to prevent detrimental effects on the biomass. The results presented here are of significance in defining the maximum threshold for bulk ethanol concentration in production media. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-24T11:15:33Z 2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/3066 |
url |
http://hdl.handle.net/10400.9/3066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, T. Lopes da; Passarinho, Paula C.; Galriça, Ricardo...[et.al.]. - Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry. In: Biotechnology Reports, Vol. 17, p. 137-147 2215-017X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130228067401728 |