Characterisation of phenolic acid derivatives and flavonoids from different morphological parts of Helichrysum obconicum by a RP-HPLC–DAD-(−)–ESI-MSn method

• Autor(a) principal: Gouveia, Sandra
• Data de Publicação: 2011
• Outros Autores: Castilho, Paula C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.13/3256
• topic: Hydroxycinnamic acids; Flavonoids; LC–MSn; Helichrysum; .; Faculdade de Ciências Exatas e da Engenharia

The phenolic composition from different morphological parts of Helichrysum obconicum was investigated for the first time and 50 different phenolic compounds were detected. Phenolic acid conjugates, mainly mono- and di-caffeoylquinic acid derivatives, were the major components; some flavonoid derivatives were also detected in small amounts. Their separation and identification was performed by a high-performance liquid chromatography/electron spray ionisation tandem ion trap mass spectrometry method, with special emphasis on MSn fragmentation. The presence of di- and tricaffeoylshikimic acid isomers in Helichrysum species extracts was reported for the first time, the spectra of these compounds were mainly characterised by the presence of a [caffeoylshikimic acid-H]- ion at m/z 335. A lamiridosins-di-O-hexoside, an unusual component in Asteraceae species, was also detected.