Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3233 |
Resumo: | The caffeoylquinic acids (CQA) found in Helichrysum species from Madeira Archipelago were identified and quantified. The HPLC-DAD–ESI/MSn method applied was developed and validated showing a good repeatability with recovery values ≥96%. The use of a RP-C18 with a small internal diameter (Ø 3.0 mm) and an acidic mobile phase (acetonitrile and water with 0.1% (v/v) of formic acid) enabled the separation of all compounds within a 30 min analysis. A good resolution between 1,5-O-diCQA and 3,5-O-diCQA isomers, usually hard to separate, was also accomplished. Dicaffeoylquinic acids isomers were the major components among the quantified hydroxycinnamic acids. 1,5-O-diCQA, 3,5-O-diCQA and 5-O-CQA were the compounds found in higher amounts for the different species. The distinct uses of these plants described in the local folk medicine can be related to the phenolic composition. |
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Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from MacaronesiaCaffeoylquinic acidsHPLC-DAD–ESI/MSHelichrysumValidation.Faculdade de Ciências Exatas e da EngenhariaThe caffeoylquinic acids (CQA) found in Helichrysum species from Madeira Archipelago were identified and quantified. The HPLC-DAD–ESI/MSn method applied was developed and validated showing a good repeatability with recovery values ≥96%. The use of a RP-C18 with a small internal diameter (Ø 3.0 mm) and an acidic mobile phase (acetonitrile and water with 0.1% (v/v) of formic acid) enabled the separation of all compounds within a 30 min analysis. A good resolution between 1,5-O-diCQA and 3,5-O-diCQA isomers, usually hard to separate, was also accomplished. Dicaffeoylquinic acids isomers were the major components among the quantified hydroxycinnamic acids. 1,5-O-diCQA, 3,5-O-diCQA and 5-O-CQA were the compounds found in higher amounts for the different species. The distinct uses of these plants described in the local folk medicine can be related to the phenolic composition.Canadian Institute of Food Science and Technology (CIFST)DigitUMaGouveia, Sandra C.Castilho, Paula C.2021-03-31T13:19:45Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3233engGouveia, S. C., & Castilho, P. C. (2012). Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia. Food research international, 45(1), 362-368.10.1016/j.foodres.2011.09.023info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-19T05:34:05Zoai:digituma.uma.pt:10400.13/3233Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:11.207001Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
title |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
spellingShingle |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia Gouveia, Sandra C. Caffeoylquinic acids HPLC-DAD–ESI/MS Helichrysum Validation . Faculdade de Ciências Exatas e da Engenharia |
title_short |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
title_full |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
title_fullStr |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
title_full_unstemmed |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
title_sort |
Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia |
author |
Gouveia, Sandra C. |
author_facet |
Gouveia, Sandra C. Castilho, Paula C. |
author_role |
author |
author2 |
Castilho, Paula C. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Gouveia, Sandra C. Castilho, Paula C. |
dc.subject.por.fl_str_mv |
Caffeoylquinic acids HPLC-DAD–ESI/MS Helichrysum Validation . Faculdade de Ciências Exatas e da Engenharia |
topic |
Caffeoylquinic acids HPLC-DAD–ESI/MS Helichrysum Validation . Faculdade de Ciências Exatas e da Engenharia |
description |
The caffeoylquinic acids (CQA) found in Helichrysum species from Madeira Archipelago were identified and quantified. The HPLC-DAD–ESI/MSn method applied was developed and validated showing a good repeatability with recovery values ≥96%. The use of a RP-C18 with a small internal diameter (Ø 3.0 mm) and an acidic mobile phase (acetonitrile and water with 0.1% (v/v) of formic acid) enabled the separation of all compounds within a 30 min analysis. A good resolution between 1,5-O-diCQA and 3,5-O-diCQA isomers, usually hard to separate, was also accomplished. Dicaffeoylquinic acids isomers were the major components among the quantified hydroxycinnamic acids. 1,5-O-diCQA, 3,5-O-diCQA and 5-O-CQA were the compounds found in higher amounts for the different species. The distinct uses of these plants described in the local folk medicine can be related to the phenolic composition. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z 2021-03-31T13:19:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3233 |
url |
http://hdl.handle.net/10400.13/3233 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gouveia, S. C., & Castilho, P. C. (2012). Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia. Food research international, 45(1), 362-368. 10.1016/j.foodres.2011.09.023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Canadian Institute of Food Science and Technology (CIFST) |
publisher.none.fl_str_mv |
Canadian Institute of Food Science and Technology (CIFST) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799129932481167360 |