Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1186/s13071-017-2072-1 |
Resumo: | Background: The taxonomic status of the brown dog tick Rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as R. sanguineus (sensu lato) until its taxonomic status is resolved. Recent genetic studies revealed the existence of more than one lineage of R. sanguineus (s.l.) in temperate countries. In this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas located in all major geographical regions of Portugal. Methods: A total of 347 ticks were collected from rural dogs living in different regions of Portugal. These ticks were morphologically identified and partial mitochondrial 16S rRNA gene sequences (~300 bp) were obtained from representative specimens. Results: The ticks were morphologically identified as Ixodes ricinus (seven males and 27 females), Rhipicephalus bursa (one male), Rhipicephalus pusillus (one female) and R. sanguineus (s.l.) (two larvae, 101 nymphs, 108 males and 100 females). Partial mitochondrial 16S rRNA gene sequences were obtained from 58 R. sanguineus (s.l.) specimens, and all of them were genetically identified as belonging to the so-called temperate lineage of R. sanguineus (s.l.) Conclusions: These results strongly suggest that the temperate species of R. sanguineus (s.l.) is the only representative of this tick group found on dogs in Portugal. It also adds weight to the hypothesis that Rhipicephalus turanicus is not present in this country, although further investigations are necessary to confirm this. |
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Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in PortugalBrown dog ticksDogsGeneticsMorphologyPortugalRhipicephalusParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingBackground: The taxonomic status of the brown dog tick Rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as R. sanguineus (sensu lato) until its taxonomic status is resolved. Recent genetic studies revealed the existence of more than one lineage of R. sanguineus (s.l.) in temperate countries. In this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas located in all major geographical regions of Portugal. Methods: A total of 347 ticks were collected from rural dogs living in different regions of Portugal. These ticks were morphologically identified and partial mitochondrial 16S rRNA gene sequences (~300 bp) were obtained from representative specimens. Results: The ticks were morphologically identified as Ixodes ricinus (seven males and 27 females), Rhipicephalus bursa (one male), Rhipicephalus pusillus (one female) and R. sanguineus (s.l.) (two larvae, 101 nymphs, 108 males and 100 females). Partial mitochondrial 16S rRNA gene sequences were obtained from 58 R. sanguineus (s.l.) specimens, and all of them were genetically identified as belonging to the so-called temperate lineage of R. sanguineus (s.l.) Conclusions: These results strongly suggest that the temperate species of R. sanguineus (s.l.) is the only representative of this tick group found on dogs in Portugal. It also adds weight to the hypothesis that Rhipicephalus turanicus is not present in this country, although further investigations are necessary to confirm this.Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)RUNDantas-Torres, FilipeMaia, CarlaLatrofa, Maria StefaniaAnnoscia, GiadaCardoso, LuísOtranto, Domenico2018-05-10T22:16:02Z2017-03-132017-03-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttps://doi.org/10.1186/s13071-017-2072-1engPURE: 3202317http://www.scopus.com/inward/record.url?scp=85014995725&partnerID=8YFLogxKhttps://doi.org/10.1186/s13071-017-2072-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:51Zoai:run.unl.pt:10362/36467Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:30.124073Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
title |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
spellingShingle |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal Dantas-Torres, Filipe Brown dog ticks Dogs Genetics Morphology Portugal Rhipicephalus Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
title_full |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
title_fullStr |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
title_full_unstemmed |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
title_sort |
Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal |
author |
Dantas-Torres, Filipe |
author_facet |
Dantas-Torres, Filipe Maia, Carla Latrofa, Maria Stefania Annoscia, Giada Cardoso, Luís Otranto, Domenico |
author_role |
author |
author2 |
Maia, Carla Latrofa, Maria Stefania Annoscia, Giada Cardoso, Luís Otranto, Domenico |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Vector borne diseases and pathogens (VBD) Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) RUN |
dc.contributor.author.fl_str_mv |
Dantas-Torres, Filipe Maia, Carla Latrofa, Maria Stefania Annoscia, Giada Cardoso, Luís Otranto, Domenico |
dc.subject.por.fl_str_mv |
Brown dog ticks Dogs Genetics Morphology Portugal Rhipicephalus Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
Brown dog ticks Dogs Genetics Morphology Portugal Rhipicephalus Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Background: The taxonomic status of the brown dog tick Rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as R. sanguineus (sensu lato) until its taxonomic status is resolved. Recent genetic studies revealed the existence of more than one lineage of R. sanguineus (s.l.) in temperate countries. In this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas located in all major geographical regions of Portugal. Methods: A total of 347 ticks were collected from rural dogs living in different regions of Portugal. These ticks were morphologically identified and partial mitochondrial 16S rRNA gene sequences (~300 bp) were obtained from representative specimens. Results: The ticks were morphologically identified as Ixodes ricinus (seven males and 27 females), Rhipicephalus bursa (one male), Rhipicephalus pusillus (one female) and R. sanguineus (s.l.) (two larvae, 101 nymphs, 108 males and 100 females). Partial mitochondrial 16S rRNA gene sequences were obtained from 58 R. sanguineus (s.l.) specimens, and all of them were genetically identified as belonging to the so-called temperate lineage of R. sanguineus (s.l.) Conclusions: These results strongly suggest that the temperate species of R. sanguineus (s.l.) is the only representative of this tick group found on dogs in Portugal. It also adds weight to the hypothesis that Rhipicephalus turanicus is not present in this country, although further investigations are necessary to confirm this. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-13 2017-03-13T00:00:00Z 2018-05-10T22:16:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1186/s13071-017-2072-1 |
url |
https://doi.org/10.1186/s13071-017-2072-1 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PURE: 3202317 http://www.scopus.com/inward/record.url?scp=85014995725&partnerID=8YFLogxK https://doi.org/10.1186/s13071-017-2072-1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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