Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

Detalhes bibliográficos
Autor(a) principal: Amorim, Lara R.P.
Data de Publicação: 2010
Outros Autores: Tamames, Juan A.C., Silva, Joana G.L., Gibbs, Paul A., Teixeira, Paula C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/4850
Resumo: This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2a, 135KP and 120 phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.
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spelling Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophagesSalmonella spp.BacteriophagesSpot testLytic spectraPhage typingCluster analysisDiscriminant analysisThis study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2a, 135KP and 120 phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaAmorim, Lara R.P.Tamames, Juan A.C.Silva, Joana G.L.Gibbs, Paul A.Teixeira, Paula C.2011-07-29T09:42:21Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/4850engAMORIM, Lara R. P... [et al.] - Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages. Food Control. ISSN 0956-7135. Vol. 21, n.º 5 (2010), p. 754-7590956-71351873-7129WOS:000274843400028info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:11:48Zoai:repositorio.ucp.pt:10400.14/4850Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:06:39.884885Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
title Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
spellingShingle Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
Amorim, Lara R.P.
Salmonella spp.
Bacteriophages
Spot test
Lytic spectra
Phage typing
Cluster analysis
Discriminant analysis
title_short Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
title_full Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
title_fullStr Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
title_full_unstemmed Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
title_sort Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages
author Amorim, Lara R.P.
author_facet Amorim, Lara R.P.
Tamames, Juan A.C.
Silva, Joana G.L.
Gibbs, Paul A.
Teixeira, Paula C.
author_role author
author2 Tamames, Juan A.C.
Silva, Joana G.L.
Gibbs, Paul A.
Teixeira, Paula C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Amorim, Lara R.P.
Tamames, Juan A.C.
Silva, Joana G.L.
Gibbs, Paul A.
Teixeira, Paula C.
dc.subject.por.fl_str_mv Salmonella spp.
Bacteriophages
Spot test
Lytic spectra
Phage typing
Cluster analysis
Discriminant analysis
topic Salmonella spp.
Bacteriophages
Spot test
Lytic spectra
Phage typing
Cluster analysis
Discriminant analysis
description This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2a, 135KP and 120 phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2011-07-29T09:42:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/4850
url http://hdl.handle.net/10400.14/4850
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv AMORIM, Lara R. P... [et al.] - Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages. Food Control. ISSN 0956-7135. Vol. 21, n.º 5 (2010), p. 754-759
0956-7135
1873-7129
WOS:000274843400028
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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