Maintaining yeast viability in continuous primary beer fermentation
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31901 |
Resumo: | Continuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright © 2014 The Institute of Brewing & Distilling |
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Maintaining yeast viability in continuous primary beer fermentationContinuous fermentationLignocellulosic yeast carrier (LCExcess of biomassFoam fractionationLignocellulosic yeast carrier (LCYC)Science & TechnologyContinuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright © 2014 The Institute of Brewing & DistillingEduardo Pires gratefully acknowledges the Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) for PhD fellowship support (SFRH/BD/61777/2009) and Cristina Ribeiro from the Centre of Molecular and Environmental Biology/Department of Biology for technical support. This work was also supported by FEDER through POFC - COMPETE and by national funds from FCT through the project PEst-C/BIA/UI4050/2011. The materials supplied by UNICER Bebidas, S.A. (S. Mamede de Infesta, Portugal) are gratefully acknowledged. The financial contributions of the EU FP7 project EcoBioCAP - Ecoefficient Biodegradable Composite Advanced Packaging, grant agreement no. 265669 as well as of the Grant Agency of the Czech Republic (project GACR P503/12/1424) are also gratefully acknowledged.Institute of Brewing & DistillingUniversidade do MinhoPires, Eduardo J.Teixeira, J. A.Brányik, TomášCôrte-Real, ManuelaVicente, A. A.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31901eng0046-975010.1002/jib.111info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:33Zoai:repositorium.sdum.uminho.pt:1822/31901Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:33.280080Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Maintaining yeast viability in continuous primary beer fermentation |
title |
Maintaining yeast viability in continuous primary beer fermentation |
spellingShingle |
Maintaining yeast viability in continuous primary beer fermentation Pires, Eduardo J. Continuous fermentation Lignocellulosic yeast carrier (LC Excess of biomass Foam fractionation Lignocellulosic yeast carrier (LCYC) Science & Technology |
title_short |
Maintaining yeast viability in continuous primary beer fermentation |
title_full |
Maintaining yeast viability in continuous primary beer fermentation |
title_fullStr |
Maintaining yeast viability in continuous primary beer fermentation |
title_full_unstemmed |
Maintaining yeast viability in continuous primary beer fermentation |
title_sort |
Maintaining yeast viability in continuous primary beer fermentation |
author |
Pires, Eduardo J. |
author_facet |
Pires, Eduardo J. Teixeira, J. A. Brányik, Tomáš Côrte-Real, Manuela Vicente, A. A. |
author_role |
author |
author2 |
Teixeira, J. A. Brányik, Tomáš Côrte-Real, Manuela Vicente, A. A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pires, Eduardo J. Teixeira, J. A. Brányik, Tomáš Côrte-Real, Manuela Vicente, A. A. |
dc.subject.por.fl_str_mv |
Continuous fermentation Lignocellulosic yeast carrier (LC Excess of biomass Foam fractionation Lignocellulosic yeast carrier (LCYC) Science & Technology |
topic |
Continuous fermentation Lignocellulosic yeast carrier (LC Excess of biomass Foam fractionation Lignocellulosic yeast carrier (LCYC) Science & Technology |
description |
Continuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright © 2014 The Institute of Brewing & Distilling |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31901 |
url |
http://hdl.handle.net/1822/31901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0046-9750 10.1002/jib.111 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132725163065344 |