Maintaining yeast viability in continuous primary beer fermentation

Detalhes bibliográficos
Autor(a) principal: Pires, Eduardo J.
Data de Publicação: 2014
Outros Autores: Teixeira, J. A., Brányik, Tomáš, Côrte-Real, Manuela, Vicente, A. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31901
Resumo: Continuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright © 2014 The Institute of Brewing & Distilling
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spelling Maintaining yeast viability in continuous primary beer fermentationContinuous fermentationLignocellulosic yeast carrier (LCExcess of biomassFoam fractionationLignocellulosic yeast carrier (LCYC)Science & TechnologyContinuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright © 2014 The Institute of Brewing & DistillingEduardo Pires gratefully acknowledges the Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) for PhD fellowship support (SFRH/BD/61777/2009) and Cristina Ribeiro from the Centre of Molecular and Environmental Biology/Department of Biology for technical support. This work was also supported by FEDER through POFC - COMPETE and by national funds from FCT through the project PEst-C/BIA/UI4050/2011. The materials supplied by UNICER Bebidas, S.A. (S. Mamede de Infesta, Portugal) are gratefully acknowledged. The financial contributions of the EU FP7 project EcoBioCAP - Ecoefficient Biodegradable Composite Advanced Packaging, grant agreement no. 265669 as well as of the Grant Agency of the Czech Republic (project GACR P503/12/1424) are also gratefully acknowledged.Institute of Brewing & DistillingUniversidade do MinhoPires, Eduardo J.Teixeira, J. A.Brányik, TomášCôrte-Real, ManuelaVicente, A. A.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31901eng0046-975010.1002/jib.111info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:33Zoai:repositorium.sdum.uminho.pt:1822/31901Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:33.280080Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Maintaining yeast viability in continuous primary beer fermentation
title Maintaining yeast viability in continuous primary beer fermentation
spellingShingle Maintaining yeast viability in continuous primary beer fermentation
Pires, Eduardo J.
Continuous fermentation
Lignocellulosic yeast carrier (LC
Excess of biomass
Foam fractionation
Lignocellulosic yeast carrier (LCYC)
Science & Technology
title_short Maintaining yeast viability in continuous primary beer fermentation
title_full Maintaining yeast viability in continuous primary beer fermentation
title_fullStr Maintaining yeast viability in continuous primary beer fermentation
title_full_unstemmed Maintaining yeast viability in continuous primary beer fermentation
title_sort Maintaining yeast viability in continuous primary beer fermentation
author Pires, Eduardo J.
author_facet Pires, Eduardo J.
Teixeira, J. A.
Brányik, Tomáš
Côrte-Real, Manuela
Vicente, A. A.
author_role author
author2 Teixeira, J. A.
Brányik, Tomáš
Côrte-Real, Manuela
Vicente, A. A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pires, Eduardo J.
Teixeira, J. A.
Brányik, Tomáš
Côrte-Real, Manuela
Vicente, A. A.
dc.subject.por.fl_str_mv Continuous fermentation
Lignocellulosic yeast carrier (LC
Excess of biomass
Foam fractionation
Lignocellulosic yeast carrier (LCYC)
Science & Technology
topic Continuous fermentation
Lignocellulosic yeast carrier (LC
Excess of biomass
Foam fractionation
Lignocellulosic yeast carrier (LCYC)
Science & Technology
description Continuous fermentation is a long known and vastly studied process. The use of immobilized cell technology (ICT) is exploited in a significant number of studies owing to the associated high volumetric productivity, time savings and low capital demand. This work was aimed at solving one of the most relevant obstacles to implementing ICT on a large scale in beer fermentations, namely the control of biomass and the maintenance of cell viability in a gas-lift bioreactor. For this purpose, foam fractionation by skimming was proposed as a tool for control of continuous biomass concentration. The consequences of foaming on lignocellulosic yeast carrier losses were assessed and discussed. A steady consumption of sugars from wort, as well as consistent ethanol production, were achieved. The viability of the suspended cells in the reactor was compared with that of the cell population in the foam using flow cytometry. Results suggest that foam might be used as a promising tool to skim non-viable biomass out of the gas-lift reactor, thus ensuring the maintenance of a cell culture with optimum viability. Copyright © 2014 The Institute of Brewing & Distilling
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31901
url http://hdl.handle.net/1822/31901
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0046-9750
10.1002/jib.111
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dc.publisher.none.fl_str_mv Institute of Brewing & Distilling
publisher.none.fl_str_mv Institute of Brewing & Distilling
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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