Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells

Detalhes bibliográficos
Autor(a) principal: Bover, Oriol
Data de Publicação: 2018
Outros Autores: Justo, Tiago, Pereira, Paulo N G, Facucho-Oliveira, João, Inácio, José M, Ramalho, José S, Domian, Ibrahim J, Belo, José António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/49616
Resumo: TJ was a pre-doctoral fellow of Fundacao para a Ciencia e Tecnologia (FCT; SFRH/BD/82280/2011), JFO (SFRH/BPD/46506/2008), JI (CEDOC/2015/36/iNOVA4Health/Multi/04462) and PNGP (SFRH/BPD/87114/2012) are FCT post-doctoral fellows. This work was supported by FCT grants (PTDC/SAU-ENB/121095/2010 and HMSP-ICT/0039/2013) to JA Belo. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia / Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged.
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spelling Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cellsSDG 3 - Good Health and Well-beingTJ was a pre-doctoral fellow of Fundacao para a Ciencia e Tecnologia (FCT; SFRH/BD/82280/2011), JFO (SFRH/BPD/46506/2008), JI (CEDOC/2015/36/iNOVA4Health/Multi/04462) and PNGP (SFRH/BPD/87114/2012) are FCT post-doctoral fellows. This work was supported by FCT grants (PTDC/SAU-ENB/121095/2010 and HMSP-ICT/0039/2013) to JA Belo. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia / Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged.Understanding the molecular pathways regulating cardiogenesis is crucial for the early diagnosis of heart diseases and improvement of cardiovascular disease. During normal mammalian cardiac development, collagen and calcium-binding EGF domain-1 (Ccbe1) is expressed in the first and second heart field progenitors as well as in the proepicardium, but its role in early cardiac commitment remains unknown. Here we demonstrate that during mouse embryonic stem cell (ESC) differentiation Ccbe1 is upregulated upon emergence of Isl1- and Nkx2.5- positive cardiac progenitors. Ccbe1 is markedly enriched in Isl1-positive cardiac progenitors isolated from ESCs differentiating in vitro or embryonic hearts developing in vivo. Disruption of Ccbe1 activity by shRNA knockdown or blockade with a neutralizing antibody results in impaired differentiation of embryonic stem cells along the cardiac mesoderm lineage resulting in a decreased expression of mature cardiomyocyte markers. In addition, knockdown of Ccbe1 leads to smaller embryoid bodies. Collectively, our results show that CCBE1 is essential for the commitment of cardiac mesoderm and consequently, for the formation of cardiac myocytes in differentiating mouse ESCs.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBover, OriolJusto, TiagoPereira, Paulo N GFacucho-Oliveira, JoãoInácio, José MRamalho, José SDomian, Ibrahim JBelo, José António2018-10-22T22:10:06Z2018-102018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/49616eng1932-6203PURE: 6047201https://doi.org/10.1371/journal.pone.0205108info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:25:14Zoai:run.unl.pt:10362/49616Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:32:14.719867Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
title Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
spellingShingle Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
Bover, Oriol
SDG 3 - Good Health and Well-being
title_short Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
title_full Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
title_fullStr Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
title_full_unstemmed Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
title_sort Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
author Bover, Oriol
author_facet Bover, Oriol
Justo, Tiago
Pereira, Paulo N G
Facucho-Oliveira, João
Inácio, José M
Ramalho, José S
Domian, Ibrahim J
Belo, José António
author_role author
author2 Justo, Tiago
Pereira, Paulo N G
Facucho-Oliveira, João
Inácio, José M
Ramalho, José S
Domian, Ibrahim J
Belo, José António
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Bover, Oriol
Justo, Tiago
Pereira, Paulo N G
Facucho-Oliveira, João
Inácio, José M
Ramalho, José S
Domian, Ibrahim J
Belo, José António
dc.subject.por.fl_str_mv SDG 3 - Good Health and Well-being
topic SDG 3 - Good Health and Well-being
description TJ was a pre-doctoral fellow of Fundacao para a Ciencia e Tecnologia (FCT; SFRH/BD/82280/2011), JFO (SFRH/BPD/46506/2008), JI (CEDOC/2015/36/iNOVA4Health/Multi/04462) and PNGP (SFRH/BPD/87114/2012) are FCT post-doctoral fellows. This work was supported by FCT grants (PTDC/SAU-ENB/121095/2010 and HMSP-ICT/0039/2013) to JA Belo. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia / Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-22T22:10:06Z
2018-10
2018-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/49616
url http://hdl.handle.net/10362/49616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 6047201
https://doi.org/10.1371/journal.pone.0205108
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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