Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/49616 |
Resumo: | TJ was a pre-doctoral fellow of Fundacao para a Ciencia e Tecnologia (FCT; SFRH/BD/82280/2011), JFO (SFRH/BPD/46506/2008), JI (CEDOC/2015/36/iNOVA4Health/Multi/04462) and PNGP (SFRH/BPD/87114/2012) are FCT post-doctoral fellows. This work was supported by FCT grants (PTDC/SAU-ENB/121095/2010 and HMSP-ICT/0039/2013) to JA Belo. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia / Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged. |
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Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cellsSDG 3 - Good Health and Well-beingTJ was a pre-doctoral fellow of Fundacao para a Ciencia e Tecnologia (FCT; SFRH/BD/82280/2011), JFO (SFRH/BPD/46506/2008), JI (CEDOC/2015/36/iNOVA4Health/Multi/04462) and PNGP (SFRH/BPD/87114/2012) are FCT post-doctoral fellows. This work was supported by FCT grants (PTDC/SAU-ENB/121095/2010 and HMSP-ICT/0039/2013) to JA Belo. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia / Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged.Understanding the molecular pathways regulating cardiogenesis is crucial for the early diagnosis of heart diseases and improvement of cardiovascular disease. During normal mammalian cardiac development, collagen and calcium-binding EGF domain-1 (Ccbe1) is expressed in the first and second heart field progenitors as well as in the proepicardium, but its role in early cardiac commitment remains unknown. Here we demonstrate that during mouse embryonic stem cell (ESC) differentiation Ccbe1 is upregulated upon emergence of Isl1- and Nkx2.5- positive cardiac progenitors. Ccbe1 is markedly enriched in Isl1-positive cardiac progenitors isolated from ESCs differentiating in vitro or embryonic hearts developing in vivo. Disruption of Ccbe1 activity by shRNA knockdown or blockade with a neutralizing antibody results in impaired differentiation of embryonic stem cells along the cardiac mesoderm lineage resulting in a decreased expression of mature cardiomyocyte markers. In addition, knockdown of Ccbe1 leads to smaller embryoid bodies. Collectively, our results show that CCBE1 is essential for the commitment of cardiac mesoderm and consequently, for the formation of cardiac myocytes in differentiating mouse ESCs.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBover, OriolJusto, TiagoPereira, Paulo N GFacucho-Oliveira, JoãoInácio, José MRamalho, José SDomian, Ibrahim JBelo, José António2018-10-22T22:10:06Z2018-102018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/49616eng1932-6203PURE: 6047201https://doi.org/10.1371/journal.pone.0205108info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:25:14Zoai:run.unl.pt:10362/49616Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:32:14.719867Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
title |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
spellingShingle |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells Bover, Oriol SDG 3 - Good Health and Well-being |
title_short |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
title_full |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
title_fullStr |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
title_full_unstemmed |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
title_sort |
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells |
author |
Bover, Oriol |
author_facet |
Bover, Oriol Justo, Tiago Pereira, Paulo N G Facucho-Oliveira, João Inácio, José M Ramalho, José S Domian, Ibrahim J Belo, José António |
author_role |
author |
author2 |
Justo, Tiago Pereira, Paulo N G Facucho-Oliveira, João Inácio, José M Ramalho, José S Domian, Ibrahim J Belo, José António |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Bover, Oriol Justo, Tiago Pereira, Paulo N G Facucho-Oliveira, João Inácio, José M Ramalho, José S Domian, Ibrahim J Belo, José António |
dc.subject.por.fl_str_mv |
SDG 3 - Good Health and Well-being |
topic |
SDG 3 - Good Health and Well-being |
description |
TJ was a pre-doctoral fellow of Fundacao para a Ciencia e Tecnologia (FCT; SFRH/BD/82280/2011), JFO (SFRH/BPD/46506/2008), JI (CEDOC/2015/36/iNOVA4Health/Multi/04462) and PNGP (SFRH/BPD/87114/2012) are FCT post-doctoral fellows. This work was supported by FCT grants (PTDC/SAU-ENB/121095/2010 and HMSP-ICT/0039/2013) to JA Belo. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia / Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-22T22:10:06Z 2018-10 2018-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/49616 |
url |
http://hdl.handle.net/10362/49616 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 PURE: 6047201 https://doi.org/10.1371/journal.pone.0205108 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137945141116928 |