Allosteric Antagonist Modulation of TRPV2 by Piperlongumine Impairs Glioblastoma Progression

• Autor(a) principal: Conde, João
• Data de Publicação: 2021
• Outros Autores: Pumroy, Ruth A., Baker, Charlotte, Rodrigues, Tiago, Guerreiro, Ana, Sousa, Bárbara B., Marques, Marta C., De Almeida, Bernardo P., Lee, Sohyon, Leites, Elvira P., Picard, Daniel, Samanta, Amrita, Vaz, Sandra H., Sieglitz, Florian, Langini, Maike, Remke, Marc, Roque, Rafael, Weiss, Tobias, Weller, Michael, Liu, Yuhang, Han, Seungil, Corzana, Francisco, Morais, Vanessa A., Faria, Cláudia C., Carvalho, Tânia, Filippakopoulos, Panagis, Snijder, Berend, Barbosa-Morais, Nuno L., Moiseenkova-Bell, Vera Y., Bernardes, Gonçalo J.L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/120531
• topic: Calcium; Conformal mapping; Medical imaging; Modulation; Self organizing maps; Tumors; Chemistry(all); Chemical Engineering(all); SDG 3 - Good Health and Well-being

Funding: We thank Sabine Baxter for assistance with hybridoma and cell culture at the University of Pennsylvania Perelman School of Medicine Cell Center Services Facility. We acknowledge the use of cryo-EM instruments at Pfizer Inc. The cryo-EM work was supported by a grant from the National Institute of Health (R01GM103899 and R01GM129357 to V.Y.M.-B.). The TRPV2-flag-RFP construct cloned into the pEGFP-N2 vector was kindly provided by Prof. Kojima (Gunma University, Japan). The Developmental Therapeutics Program is acknowledged for screening PL against the NCI-60 cancer cell line panel. We are grateful to Carlos Custódia, Eunice Paisana, and Dr. Joao Barata for their assistance with cell-line modulation ̃ and the GBM tumor model, and to Dr. Vikki Cantrill for her assistance with the editing of this manuscript. Figure design by Claudia Flandoli (draw.science). The Histology and Comparative Pathology Laboratory of the Instituto de Medicina Molecular is acknowledged for technical support. We also thank FCT Portugal (FCT Investigator IF/00624/2015 to G.J.L.B., IF/01693/2014 to V.A.M. and IF/00595/2014 to N.L.B.-M., CEECIND/00436/2018 to N.L.B.-M., PhD studentship SFRH/BD/143583/2019 to B.B.S., PhD studentship PD/BD/128289/2017 to E.P.L., Postdoctoral fellowship SFRH/BPD/1187731/2016 to M.C.M.), Agencia Estatal Investigación of Spain (AEI, RTI-2018-099592-B-C21 to F.C.), EMBO (Installation Grant 3057 to N.L.B.-M.), and the Medical Research Council (MR/N010051/1 to P.F.). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement 630731, 675007, 807281, and 743640. G.J.L.B. is a Royal Society University Research Fellow (URF\R\180019).