The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury

Detalhes bibliográficos
Autor(a) principal: Perlikowska, Renata
Data de Publicação: 2022
Outros Autores: Silva, Joana, Alves, Celso, Susano, Patrícia, Pedrosa, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8753
Resumo: This research was funded by the National Science Center (NCN, Miniatura, No DEC-2021/05/X/ST5/00294), PolishNationalAgency forAcademic Exchange (BPN/BPT/2021/1/00053), the Portuguese Foundation for Science and Technology (FCT) through the projects granted to MARE—Marine and Environmental Sciences Centre (UIDP/04292/2020 and UIDB/04292/2020), Associate Laboratory ARNET (LA/P/0069/2020) and CROSS-ATLANTIC (PTDC/BIA-OUT/29250/2017) project, co-financed by European Regional Development Fund (FEDER), through the Operational-Programme for Competitiveness and Internationalization (COMPETE 2020). This study was also supported by Programme for Cooperation in Science between Portugal and Poland through project NEURONS—Natural products as key drivers in the development of nEUropROtective therapeutic ageNtS (FCT/DRI/PNAAE 2021.09608.CBM).
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spelling The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injuryOpioid peptides6-hydroxydopamineNeuronal survivalNeuroprotectionAntioxidant activityApoptosisThis research was funded by the National Science Center (NCN, Miniatura, No DEC-2021/05/X/ST5/00294), PolishNationalAgency forAcademic Exchange (BPN/BPT/2021/1/00053), the Portuguese Foundation for Science and Technology (FCT) through the projects granted to MARE—Marine and Environmental Sciences Centre (UIDP/04292/2020 and UIDB/04292/2020), Associate Laboratory ARNET (LA/P/0069/2020) and CROSS-ATLANTIC (PTDC/BIA-OUT/29250/2017) project, co-financed by European Regional Development Fund (FEDER), through the Operational-Programme for Competitiveness and Internationalization (COMPETE 2020). This study was also supported by Programme for Cooperation in Science between Portugal and Poland through project NEURONS—Natural products as key drivers in the development of nEUropROtective therapeutic ageNtS (FCT/DRI/PNAAE 2021.09608.CBM).Peptides have revealed a large range of biological activities with high selectivity and efficiency for the development of new drugs, including neuroprotective agents. Therefore, this work investigates the neuroprotective properties of naturally occurring peptides, endomorphin-1 (EM-1), endomorphin-2 (EM-2), rubiscolin-5 (R-5), and rubiscolin-6 (R-6). We aimed at answering the question of whether wellknown opioid peptides can counteract cell injury in a common in vitromodel of Parkinson’s disease (PD). Antioxidant activity of these four peptides was evaluated by the 2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity, oxygen radical absorbance capacity (ORAC), and ferric-reducing antioxidant power (FRAP) assays, while neuroprotective effects were assessed in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y). The mechanisms associated with neuroprotection were investigated by the determination of mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, and Caspase-3 activity. Among the tested peptides, endomorphins significantly prevented neuronal death induced by 6-OHDA treatment, decreasing MMP (EM-1) or Caspase-3 activity (EM-2). Meanwhile, R-6 showed antioxidant potential by FRAP assay and exhibited the highest capacity to recover the neurotoxicity induced by 6-OHDA via attenuation of ROS levels and mitochondrial dysfunction. Generally, we hypothesize that peptides’ ability to suppress the toxic effect induced by 6-OHDA may be mediated by different cellular mechanisms. The protective effect caused by endomorphins results in an antiapoptotic effect (mitochondrial protection and decrease in Caspase-3 activity), while R-6 potency to increase a cell’s viability seems to be mediated by reducing oxidative stress. Our results may provide new insight into neurodegeneration and support the short peptides as a potent drug candidate to treat PD. However, further studies should be conducted on the detailed mechanisms of how tested peptides could suppress neuronal injuries.MDPIIC-OnlinePerlikowska, RenataSilva, JoanaAlves, CelsoSusano, PatríciaPedrosa, Rui2023-09-04T11:54:56Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8753engPerlikowska, R.; Silva, J.; Alves, C.; Susano, P.; Pedrosa, R. The Therapeutic Potential of Naturally Occurring Peptides in Counteracting SH-SY5Y Cells Injury. Int. J. Mol. Sci. 2022, 23, 11778. https://doi.org/ 10.3390/ijms2319117781422-006710.3390/ijms231911778info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-26T18:26:21Zoai:iconline.ipleiria.pt:10400.8/8753Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-26T18:26:21Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
title The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
spellingShingle The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
Perlikowska, Renata
Opioid peptides
6-hydroxydopamine
Neuronal survival
Neuroprotection
Antioxidant activity
Apoptosis
title_short The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
title_full The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
title_fullStr The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
title_full_unstemmed The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
title_sort The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury
author Perlikowska, Renata
author_facet Perlikowska, Renata
Silva, Joana
Alves, Celso
Susano, Patrícia
Pedrosa, Rui
author_role author
author2 Silva, Joana
Alves, Celso
Susano, Patrícia
Pedrosa, Rui
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Perlikowska, Renata
Silva, Joana
Alves, Celso
Susano, Patrícia
Pedrosa, Rui
dc.subject.por.fl_str_mv Opioid peptides
6-hydroxydopamine
Neuronal survival
Neuroprotection
Antioxidant activity
Apoptosis
topic Opioid peptides
6-hydroxydopamine
Neuronal survival
Neuroprotection
Antioxidant activity
Apoptosis
description This research was funded by the National Science Center (NCN, Miniatura, No DEC-2021/05/X/ST5/00294), PolishNationalAgency forAcademic Exchange (BPN/BPT/2021/1/00053), the Portuguese Foundation for Science and Technology (FCT) through the projects granted to MARE—Marine and Environmental Sciences Centre (UIDP/04292/2020 and UIDB/04292/2020), Associate Laboratory ARNET (LA/P/0069/2020) and CROSS-ATLANTIC (PTDC/BIA-OUT/29250/2017) project, co-financed by European Regional Development Fund (FEDER), through the Operational-Programme for Competitiveness and Internationalization (COMPETE 2020). This study was also supported by Programme for Cooperation in Science between Portugal and Poland through project NEURONS—Natural products as key drivers in the development of nEUropROtective therapeutic ageNtS (FCT/DRI/PNAAE 2021.09608.CBM).
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
2023-09-04T11:54:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8753
url http://hdl.handle.net/10400.8/8753
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Perlikowska, R.; Silva, J.; Alves, C.; Susano, P.; Pedrosa, R. The Therapeutic Potential of Naturally Occurring Peptides in Counteracting SH-SY5Y Cells Injury. Int. J. Mol. Sci. 2022, 23, 11778. https://doi.org/ 10.3390/ijms231911778
1422-0067
10.3390/ijms231911778
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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