Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/37513 |
Resumo: | MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications. |
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Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from VibrioExopolysaccharideHyaluronic-likeNMR spectroscopyPhysicochemical propertiesBacterial matsVibrio spMO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.Elsevier2023-05-05T07:53:36Z2019-10-15T00:00:00Z2019-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37513eng0144-861710.1016/j.carbpol.2019.114999Martin-Pastor, ManuelFerreira, Andreia S.Moppert, XavierNunes, CláudiaCoimbra, Manuel A.Reis, Rui L.Guezennec, JeanNovoa-Carballal, Ramoninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:12:31Zoai:ria.ua.pt:10773/37513Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:08:08.356016Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
title |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
spellingShingle |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio Martin-Pastor, Manuel Exopolysaccharide Hyaluronic-like NMR spectroscopy Physicochemical properties Bacterial mats Vibrio sp |
title_short |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
title_full |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
title_fullStr |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
title_full_unstemmed |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
title_sort |
Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
author |
Martin-Pastor, Manuel |
author_facet |
Martin-Pastor, Manuel Ferreira, Andreia S. Moppert, Xavier Nunes, Cláudia Coimbra, Manuel A. Reis, Rui L. Guezennec, Jean Novoa-Carballal, Ramon |
author_role |
author |
author2 |
Ferreira, Andreia S. Moppert, Xavier Nunes, Cláudia Coimbra, Manuel A. Reis, Rui L. Guezennec, Jean Novoa-Carballal, Ramon |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Martin-Pastor, Manuel Ferreira, Andreia S. Moppert, Xavier Nunes, Cláudia Coimbra, Manuel A. Reis, Rui L. Guezennec, Jean Novoa-Carballal, Ramon |
dc.subject.por.fl_str_mv |
Exopolysaccharide Hyaluronic-like NMR spectroscopy Physicochemical properties Bacterial mats Vibrio sp |
topic |
Exopolysaccharide Hyaluronic-like NMR spectroscopy Physicochemical properties Bacterial mats Vibrio sp |
description |
MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-15T00:00:00Z 2019-10-15 2023-05-05T07:53:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/37513 |
url |
http://hdl.handle.net/10773/37513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0144-8617 10.1016/j.carbpol.2019.114999 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137735076741120 |