Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

Detalhes bibliográficos
Autor(a) principal: Da Costa Ribeiro, Ana Paula
Data de Publicação: 2018
Outros Autores: Anbu, S, Alegria, Elisabete, Fernandes, Alexandra, Baptista, Pedro, Mendes, Rita, Matias, A. S., Mendes, M., Guedes Da Silva, M. Fátima C., Pombeiro, Armando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/9121
Resumo: Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
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spelling Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticlesGreen chemistrySilver nanoparticlesInteractionCytotoxicityCancer cell linesSilver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.ElsevierRCIPLDa Costa Ribeiro, Ana PaulaAnbu, SAlegria, ElisabeteFernandes, AlexandraBaptista, PedroMendes, RitaMatias, A. S.Mendes, M.Guedes Da Silva, M. Fátima C.Pombeiro, Armando2018-12-03T12:02:02Z2018-052018-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/9121engRIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-1440753-332210.1016/j.biopha.2018.02.069info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:57:26Zoai:repositorio.ipl.pt:10400.21/9121Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:17:45.739671Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
title Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
spellingShingle Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
Da Costa Ribeiro, Ana Paula
Green chemistry
Silver nanoparticles
Interaction
Cytotoxicity
Cancer cell lines
title_short Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
title_full Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
title_fullStr Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
title_full_unstemmed Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
title_sort Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
author Da Costa Ribeiro, Ana Paula
author_facet Da Costa Ribeiro, Ana Paula
Anbu, S
Alegria, Elisabete
Fernandes, Alexandra
Baptista, Pedro
Mendes, Rita
Matias, A. S.
Mendes, M.
Guedes Da Silva, M. Fátima C.
Pombeiro, Armando
author_role author
author2 Anbu, S
Alegria, Elisabete
Fernandes, Alexandra
Baptista, Pedro
Mendes, Rita
Matias, A. S.
Mendes, M.
Guedes Da Silva, M. Fátima C.
Pombeiro, Armando
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Da Costa Ribeiro, Ana Paula
Anbu, S
Alegria, Elisabete
Fernandes, Alexandra
Baptista, Pedro
Mendes, Rita
Matias, A. S.
Mendes, M.
Guedes Da Silva, M. Fátima C.
Pombeiro, Armando
dc.subject.por.fl_str_mv Green chemistry
Silver nanoparticles
Interaction
Cytotoxicity
Cancer cell lines
topic Green chemistry
Silver nanoparticles
Interaction
Cytotoxicity
Cancer cell lines
description Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-03T12:02:02Z
2018-05
2018-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/9121
url http://hdl.handle.net/10400.21/9121
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv RIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-144
0753-3322
10.1016/j.biopha.2018.02.069
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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